%A Yan-hong Hu​, Ming-xin Qi​, Xiu-rong Huang​, Lan Ma​, Jing Yan​, Zheng-zheng Wu​ %T Signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell induced by recombinant human epidermal growth factor %0 Journal Article %D 2006 %J Journal of Integrative Medicine %R 10.3736/jcim20060111 %P 39-42 %V 4 %N 1 %U {http://www.jcimjournal.com/CN/abstract/article_1889.shtml} %8 2006-01-20 %X

Objective

To investigate the signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell (LEC) induced by recombinant human epidermal growth factor (rhEGF).

Methods

There were three groups in this experiment, which were normal control group, untreated group and curcumin-treated group. Proliferation of LEC was induced by rhEGF (50 μg/L). The concentration of intracellular Ca2+ ([Ca2+]i) in LEC was measured with Fura-2/AM by spectrofluorimetry. The contents of intracellular cAMP and cGMP were assayed by radioimmunoassay.

Results

The [Ca2+]i in LEC was obviously increased in the untrated group as compared with that in the normal control group (P<0.01), and the [Ca2+]i in LEC in the curcumin-treated group was highest among three groups (P<0.01). The content of intracellular cAMP in LEC was decreased while the content of intracellular cGMP was obviously increased in the untreated group as compared with those in the normal control group (P<0.01). The content of intracellular cAMP in LEC was higher in the curcumin-treated group than that in the untreated group, while the content of intracellular cGMP was lower than that in the untreated group (P<0.01). 

Conclusion

The antiproliferation effects of curcumin on LEC may relate to the regulations of multiple processes of signal transduction.