%A Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du %T Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism %0 Journal Article %D 2010 %J Journal of Integrative Medicine %R 10.3736/jcim20101108 %P 1048-1052 %V 8 %N 11 %U {http://www.jcimjournal.com/CN/abstract/article_35.shtml} %8 2010-11-20 %X

Objective

Cell senescence is an important anti-cancer mechanism and may contribute to cancer therapeutic outcome. The present study observed the effects of Tenglong Buzhong Decoction (TLBZD), a Chinese herbal formula, on senescence in human colon carcinoma LS-174-T cells.

Methods

LS-174-T cells were treated with TLBZD, and morphology change was observed under a microscope. Cell senescence was identified by senescence-associated β-galactosidase (SA-β-gal) staining, and cell cycle was analyzed by flow cytometry. Expressions of p53, p21 WAF1/CIP1, p16 and RB and RB phosphorylation were detected by Western blot.

Results

After being treated with TLBZD, LS-174-T cells became enlarged and flattened by morphology; SA-β-gal staining was positive and cell cycle was arrested in G0/G1. In addition, up-regulations of p21 WAF1/CIP1 and p16, as well as inhibition of RB phosphorylation were detected in response to TLBZD treatment. Expressions of p53 and RB were unchanged after TLBZD treatment.

Conclusion

TLBZD is effective in inducing cell senescence in human colon carcinoma LS-174-T cells, which may relate to up-regulations of p21 WAF1/CIP1 and p16 and inhibition of RB phosphorylation.