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Journal of Chinese Integrative Medicine: 2008; 6(4): 355-360
DOI: 10.3736/jcim20080406
Investigation of gene expression profiles in patients with blood stasis syndrome
1. Xiao-juan MA (Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China )
2. Hui-jun YIN (Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China E-mail: huijunyin@yahoo.com.cn)
3. Ke-ji CHEN (Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China )

Objective: To investigate the differential gene expression profiles in patients with blood stasis syndrome by oligonucleotide microarray technique.

Methods: Sixteen patients with blood stasis syndrome were divided into patients with coronary heart disease (CAD) (n=8) and non-CAD patients (n=8) by using coronary angiography. The sex- and age-matched eight healthy persons were enrolled as control group. Venous bloods were collected for extracting RNA. Test-3 chip was first employed to examine the quality of samples. Then the samples were hybridized with Affymetrix U133 Plus 2.0 array to compare the gene expression profiles among the three groups. Gene-array scanner and gene chip operating software were applied to screen hybridization signals and analyze gene expression respectively. Based on the comparison of the three groups of samples, the differential genes related with blood stasis syndrome were analyzed by Gene Ontology (GO) and pathway, and confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR).

Results: Forty-eight differential genes were found being associated with blood stasis syndrome, including 26 up-regulated genes and 22 down-regulated genes. Five of the forty-eight genes (10.4%) were related to inflammatory reaction and immune response through the GO analysis. In the pathway analysis, five of ten significant pathways were referred to inflammation and immune response. The results of real-time RT-PCR proved the accuracy of the gene chip.

Conclusion: Inflammatory- and immune-related genes have a remarkable predominance in blood stasis syndrome gene expression profiles, which may explain the function of inflammation and immune response in the occurrence and development of blood stasis syndrome.

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Please cite this article as:
Ma XJ, Yin HJ, Chen KJ. Investigation of gene expression profiles in patients with blood stasis syndrome. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2008; 6(4): 355-360.
References:
1Chen KJ, Shi ZX. Practical workbook of blood-stasis syndrome[M]. Beijing: People's Medical Publishing House, 1999. 5-8. Chinese.
2Xu H, Lu XY, Chen KJ, et al. Study on correlation of blood-stasis syndrome and its accompanied syndromes with pathological changes showed in coronary angiography and restenosis after percutaneous coronary intervention[J].Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007, 27(1): 8-13. Chinese with abstract in English.
3Zhong S, Storch KF, Lipan O, et al. GoSurfer: a graphical interactive tool for comparative analysis of large gene sets in Gene Ontology space[J].Appl Bioinformatics, 2004, 3(4): 261-264.  .
4Shi YH, Zhu SW, Mao XZ, et al. Transcriptome profiling, molecular biological and physiological studies reveal a major role for ethylene in cotton fiber cell elongation[J].Plant Cell, 2006, 18(3): 651-664.  .
5Mao X, Cai T, Olyarchuk JG, et al. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary[J].Bioinformatics, 2005, 21(15): 3787-3793.  .
6Mlecnik B, Scheideler M, Hackl H, et al. Pathway Explorer: web service for visualizing high-throughput expression data on biological pathways[J].Nucleic Acids Res, 2005, 33(Web Server issue): 633-637.  .
7Ma M, Zhang GJ. Research progress of blood stasis syndrome objectivity[J].Shandong Zhong Yi Yao Da Xue Xue Bao, 2002, 2(26): 155-158. Chinese.
8Ma XJ, Yin HJ, Chen KJ. Research progress of correlation between blood-stasis syndrome and inflammation[J].Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007, 27(7): 669-672. Chinese with abstract in English.
9Ferroni P, Martini F, Cardarello CM, et al. Enhanced interleukin-1beta in hypercholesterolemia: effects of simvastatin and low-dose aspirin[J].Circulation, 2003, 108(14): 1673-1675.  .
10Tang YH, Zhang SP, Liang Y, et al. Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1β, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats[J]. Zhong Xi Yi Jie He Xue Bao, 2007, 5(3): 328-332. Chinese with abstract in English.
11Burger PC, Wagner DD. Platelet P-selectin facilitates atherosclerotic lesion development[J].Blood, 2003, 101(7): 2661-2666.  .
12Nannizzi-Alaimo L, Alves VL, Phillips DR. Inhibitory effects of glycoprotein IIb/IIIa antagonists and aspirin on the release of soluble CD40 ligand during platelet stimulation[J].Circulation, 2003, 107(8): 1123-1128.  .
13Jiang H, Du M, Xi Y, et al. Genetic polymorphism of FcγRⅡA-genes in patients with coronary heart disease and dilated cardiomyopathy[J].Guo Ji Yi Chuan Xue Za Zhi, 2006, 29(4): 246-248. Chinese with abstract in English.
14Konishi H, Katoh Y, Takaya N, et al. Platelets activated by collagen through immunoreceptor tyrosine-based activation motif play pivotal role in initiation and generation of neointimal hyperplasia after vascular injury[J].Circulation, 2002, 105(8): 912-916.  .

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