|
 Services |
|
|
 |
Subscribe |
|
|
|
|
|
|
Response |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Information
For |
|
|
|
|
|
|
|
|
| About
JCIM |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Journal of Chinese Integrative Medicine: 2005; 3(4): 307-310
DOI: 10.3736/jcim20050417 |
|
| Effect of Xingding Injection on expression of heat shock protein 72 in vascular endothelial cells |
| 1. | LI Jing (Department of Emergency, Hubei General Hospital of Chinese People's Armed Police Forces, Wuhan, Hubei Province 430060, China E-mail: iwyyc@hotmail.com) | | 2. | WU Yun-Xia (Institute of Integrated Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, China ) | | 3. | ZHANG Mei-Chun (Department of Internal Medicine, Hubei General Hospital of Chinese People's Armed Police Forces, Wuhan, Hubei Province 430060, China ) |
|
|
Objective: To study the effect of Xingding Injection on the expression of heat shock protein 72 (HSP 72) in vascular endothelial cells after ultraviolet radiation.
Methods: Porcine aortic endothelial cells were cultured for 72 hours in culture mediums with different concentrations (0.1, 0.5, 1.0, 5.0, 10 mg/ml) of Xingding Injection. Ultraviolet radiation was administered to the cultured cells for 30 minutes. Western-blot assay was used to measure the expression of HSP 72 in the vascular endothelial cells.
Results: There was no expression of HSP 72 in the cultured vascular endothelial cells without ultraviolet radiation, but there was some expression of HSP 72 after ultraviolet radiation. Xingding Injection of different concentrations could significantly improve the expression of HSP 72. The expression of HSP 72 in the vascular endothelial cells cultured in culture medium with 1.0 mg/ml Xingding Injection was the highest, and there was no more increase of expression when the concentration was higher, instead the expression decreased.
Conclusion: Xingding Injection can protect the vascular endothelial cells from injury during stress. It may be one of its mechanisms in preventing and treating cardio-cerebrovascular disorders. |
|
| Welcome to JCIM! You are the number
2466
reader of this article! |
|
| Download Article: |
| [Full Text] [PDF] [Chinese] [Previous] [Next] [This Issue] |
|
| Please cite this article as: |
| Li J, Wu YX, Zhang MC. Effect of Xingding Injection on expression of heat shock protein 72 in vascular endothelial cells. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2005; 3(4): 307-310. |
|
| References:
|
| 1 | Ranson NA, White HE, Sailbil HR. Chaperonins[J].Biochem J, 1998, 333(Pt 2): 233-242. . |
| 2 | Gerge J, Shoenfeld Y, Georgopoulos C. Heat shock proteins and stress tolerance in the biology of heat shock proteins and molecular chaperones[J].Arteri Thromb Vasc Biol, 1999, 19(3): 505-510. . |
| 3 | Angelidis CE, Lazaridis I, Pagoulatos GN. Constitutive expression of heat-shock protein 70 in mammalian cells confers thermoresistance[J].Eur J Biochem, 1991, 199(1): 35-39. . |
| 4 | Rajalingam R, Mehra NK, Singal DP. Polymorphism in heat-shock protein 70-1 (HSP 70-1) gene promoter region and susceptibility to tubercloid leprosy and pulmonary tuberculosis in Asian Indians[J].Indian J Exp Biol, 2000, 38(7): 658-662. . |
| 5 | Slater DN, Sloan JM. The porcine endothelial cell in tissue culture[J].Atherosclerosis, 1975, 21(2): 259-272. . |
| 6 | Basta G, Venneri L, Lazzerini G, et al. In vitro modulation of intracellular oxidative stress of endothelial cells by diagnostic cardiac ultrasound[J].Cardiovasc Res, 2003, 58(1): 156-161. . |
| 7 | Yoshida H, Haze K, Yanagi H, et al. Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors[J].J Biol Chem, 1998, 273(50): 33741-33749. . |
| 8 | Chu B, Soncin F, Price BD, et al. Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1[J].J Biol Chem, 1996, 271(48): 30847-30857. . |
|
|
|
|
|
|