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Journal of Chinese Integrative Medicine: 2006; 4(1): 43-47
DOI: 10.3736/jcim20060112
Effects of Tangshenling Mixture and benazepril on rats with diabetic nephropathy and its mechanism
1. Xue-Lin HE (Nephrology Center, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China E-mail: hexuelin88@hotmail.com)
2. Jian-Ping LI (Department of Traditional Chinese Medicine, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China )
3. Yi-Ping CHEN (Department of Nephrology, Longhua Hospital, Shanghai University of Traditonal Chinese Medicine, Shanghai 200032, China )
4. Zhi-Gang ZHANG (Department of Pathology, Medical School, Fudan University, Shanghai 200032, China )
5. Wei-Qin LIN (Nephrology Center, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China )
6. Jiang-Hua CHEN (Nephrology Center, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China )

Objective: To investigate the effects of Tangshenling Mixture (TSLM) and benazepril on rats with diabetic nephropathy (DN) and its mechanism.

Methods: Diabetic nephropathy was induced in rats by intraperitoneal injection of streptozotocin. Fifty-eight rats with DN were randomly divided into four groups: untreated group, TSLM-treated group, TSLM plus benazepril-treated group and benazepril-treated group. Another seven normal rats were included in normal control group. Then, rats in each group were accordingly given normal saline, TSLM, TSLM plus benazepril and benazepril orally for six weeks respectively. Blood and urine biochemical indexes, plasma atrial natriuretic factor (ANF), pathomorphology of renal tissue, transforming growth factor beta1 (TGF-β1) and glucose transporter 1 (GLUT1) mRNAs in renal tissue were observed.

Results: Both TSLM and benazepril could decrease urinary albumin excretion rates, creatinine clearance and ratio of kidney weight to body weight of the rats with DN as well as reduce the pathological damages of the renal tissues. TSLM could reduce the level of plasma ANF and the expression of GLUT1 mRNA, but had no significant effect on the expression of TGF-β1 mRNA. Benazepril could reduce the expression of TGF-β1 mRNA, but had no significant effect on plasma ANF and the expression of GLUT1 mRNA.

Conclusion: TSLM can reduce the pathological damages of renal tissues in rats with early-stage DN, and its mechanism may relate to decreasing the level of plasma ANF and the expression of GLUT1 mRNA which is different from that of benazepril. It seems that TSLM has synergetic effect with benazepril.

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Please cite this article as:
He XL, Li JP, Chen YP, Zhang ZG, Lin WQ, Chen JH. Effects of Tangshenling Mixture and enazepril on rats with diabetic nephropathy and its mechanism. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2006; 4(1): 43-47.
References:
1林善锬. 糖尿病肾病研究的几个关键[J].中华肾脏病杂志, 2000, 16(2): 69-70.  .
2Gilbert RE, Cox A, Wu LL, et al. Expression of transforming growth factor-beta1 and type Ⅳ collagen in the renal tubulointerstitium in experimental diabetes: effects of ACE inhibition[J].Diabetes, 1998, 47(3): 414-422.  .
3Wadhwani KC, Fukuyama R, Giordano T, et al. Quantitative reverse transcriptase-polymerase chain reaction of glucose transporter 1 mRNA levels in rat brain microvessels[J].Anal Biochem, 1993, 215(1): 134-141.  .
4Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction[J].Anal Biochem, 1987, 162(1): 156-159.  .
5贺学林, 陈以平. 糖尿病肾病的中医药临床治疗概况[J].实用中医内科杂志, 2000, 14(2): 1-3.  .
6王克勤. 牛蒡子治疗肾性蛋白尿[J].中医杂志, 1997, 38(10): 581-582.  .
7贺学林, 章素云, 陈以平, 等. 牛蒡子防治STZ糖尿病大鼠早期肾脏病变的实验研究[J].浙江中医杂志, 2003, 38(2): 88-90.  .
8胡仲仪, 陈以平, 沈玲妹, 等. 黄芪注射液治疗糖尿病肾病性水肿疗效观察[J].中国中西医结合杂志, 2000, 20(8): 618-619.  .
9祁忠华, 林善锬, 黄宇峰. 黄芪改善糖尿病早期肾血流动力学异常的研究[J].中国糖尿病杂志, 1999, 7(3): 147-149.  .
10Pozniak J. Regulation of natriuresis in diabetic nephropathy[J].Ann Acad Med Stetin, 2000, 46(2): 241-252.  .
11Gambaro G, Ceol M, Del Prete D, et al. GLUT-1 and TGF-beta: the link between hyperglycaemia and diabetic nephropathy[J].Nephrol Dial Transplant, 2000, 15(9): 1476-1477.  .
12Ziyadeh FN. Mediators of diabetic renal disease: the case for TGF-beta as the major mediator[J].J Am Soc Nephrol, 2004, 15(Suppl 1): S55-S77.  .
13He LQ, Cao HX, Shen YJ. Effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy[J]. Zhong Xi Yi Jie He Xue Bao, 2003, 1(2): 119-121.  .
14Zhou XJ, Shen JX, Huang L. Study of the mechanism of preventing proteinuria by Yishen Gujing Decoction (YSGJD) in early diabetic nephropathy[J]. Zhong Xi Yi Jie He Xue Bao, 2003, 1(1): 39-41.  .
15Wu K, Setty S, Mauer SM, et al. Altered kidney matrix gene expression in early stages of experimental diabetes[J].Acta Anat (Basel), 1997, 158(3): 155-165.  .

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