Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese Updated Monday, October 22, 2018
 Services
Submit a manuscript
Subscribe
Individuals
Institutions
Response
Send response
Read responses
Reprint
Advance online publication
Sign up for e-alert
JCIM job opportunities
Contact JCIM
 Information For
Readers
Authors
Reviewers
Advertisers
 About JCIM
Journal description
Editorial policy
Privacy policy
Copy right
Editorial staff
 Language Polishing
Journal of Chinese Integrative Medicine: 2004; 2(5): 367-371
DOI: 10.3736/jcim20040517
Effect of Bugu Mixture on all-trans retinoic acid-induced apoptosis of bone marrow stromal cells
1. LI Nan (Department of Orthopedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China E-mail: linan000@tom.com)
2. WANG He-Ming (Department of Orthopedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China )
3. LIN Xu (Molecular Medicine Center, Fujian Medical University, Fuzhou, Fujian Province 350004, China )
4. ZHENG Liang-Pu (Medical Science Experiment Center, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China )
5. SHEN Lin (Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China )
6. LIN Yi-Ping (Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China )
7. ZHOU Lin-Ying (Department of Traditional Chinese Basic Medicine, Fujian Academy of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China )
8. ZHONG Xiu-Rong (Department of Electron Microscope, Fujian Medical University, Fuzhou, Fujian Province 350004, China )
9. LIN Fang (Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou, Fujian Province 350005, China )

Objective: We used the SD rat's bone marrow stromal cells (BMSCs) cultured in vitro to observe the effects of Bugu Mixture on the apoptosis and to explore the molecular biologic mechanism of the treatment of osteoporosis with Bugu Mixture.

Methods: BMSCs were separated from the bones of the extremities of SD rats in vitro. The morphologic changes, the apoptosis cell cycles, the mitochondrion membrane potential changes, and the Bcl-2 and Bax gene expression were observed, and the effects of Bugu Mixture on the course of cell apoptosis were evaluated.

Results: The earlier use of Bugu Mixture could decrease the cells blocked in G0/G1 phase, and promote their synthesis of DNA in S phase. The expression of Bcl-2 was higher in the Bugu Mixture group than that in the all-trans retinoic acid(ATRA) induced group, and the expression of Bax was lower in the Bugu Mixture group than that in the ATRA induced group. The mitochondrion membrane potential descended significantly in the Bugu Mixture group than that in the ATRA induced group.

Conclusion: The mechanism of the treatment of osteoporosis with Bugu Mixture is that the earlier use of Bugu Mixture can decrease the amount of apoptostic cells induced by ATRA, thus promoting the cell mitosis and restraining the apoptosis. It can also act as a protector to Bcl-2 located on the mitochondrion membrane. By preventing the transferring of the Bax protein from cell-plasm to mitochondrion membrane, it takes the advantage of Bcl-2 in forming Bcl-2/Bax homodimer so as to prevent the opening of the permeability transition pore to avoid the changing of mitochondrion membrane potential and the destruction of biosynthesis caused by the mitochondrion release of apoptosis inducing factors and to reach the objective of restraining apoptosis.

Welcome to JCIM! You are the number 17490 reader of this article!
Download Article:
[Full Text]      [PDF]      [Chinese]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Li N, Wang HM, Lin X, Zheng LP, Shen L, Lin YP, Zhou LY, Zhong XR, Lin F. Effect of Bugu Mixture on all-trans retinoic acid-induced apoptosis of bone marrow stromal cells. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2004; 2(5): 367-371.
References:
1席越, 王戈平, 黄啸原. 骨组织病理解剖学技术[M]. 第1版. 北京: 人民卫生出版社, 1997. 63-64.  .
2彭黎明,王曾礼. 细胞凋亡的基础与临床[M]. 第1版. 北京: 人民卫生出版社, 2000. 171-172.  .
3Li PF, Dietz R, von Harsdorf R. p53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by Bcl-2[J].EMBO J, 1999, 18(21): 6027-6036.  .
4Cheng EH, Kirsch DG, Clem RJ, et al. Conversion of Bcl-2 to a Bax-like death effector by caspases[J].Science, 1997, 278(5345): 1966-1968.  .
5Reed JC. Double identity for proteins of the Bcl-2 family[J].Nature, 1997, 387(6635): 773-776.  .

 Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese
Copyright © 2003-2012 by JCIM Press. All rights reserved. ISSN 1672-1977