Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese Updated Saturday, September 22, 2018
Submit a manuscript
Send response
Read responses
Advance online publication
Sign up for e-alert
JCIM job opportunities
Contact JCIM
 Information For
 About JCIM
Journal description
Editorial policy
Privacy policy
Copy right
Editorial staff
 Language Polishing
Journal of Chinese Integrative Medicine: 2009; 7(1): 41-47
DOI: 10.3736/jcim20090106
Regulatory mechanism of malignant behavior of endometriosis mediated by puerarin
1. Chao-qin YU (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China E-mail: chqyu81@gmail.com)
2. Jin YU (Department of Integrated Traditional Chinese and Western Medicine, Hospital of Gynecology and Obstetrics, Fudan University, Shanghai 200011, China )
3. Jie HAN (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China )
4. Qiao-ling ZHOU (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China )
5. Wei SHEN (Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China )

Objective: To observe the inhibitory effects of puerarin on angiopoiesis of endometriotic tissue, and to explore the regulatory effects of puerarin on tumor-related gene expression of endometriosis.

Methods:The regulatory effects of puerarin on endometriotic angiopoiesis and tumor-related gene expression were observed by using a chicken chorioallantoic membrane model and gene array method.

Results: Chicken chorioallantoic membrane experiment indicated that puerarin obviously inhibited endometriotic vasiculation and angiopoiesis. The area of blood vessels was significantly reduced as compared with the untreated group (P<0.05). The expressions of oncogenes and genes related to adhesion, invasion, and apoptosis, including ERBB2, ETS2, FOS, S100A4, TEK, TERT, NFKBIA, CDH1, CD44, ITGA6, NCAM1, MMP1, FLT1, AKT1, BCL2L and BIRC5 genes, were obviously higher, while the expressions of the anti-oncogenes, anti-apoptosis genes and anti-invasion genes, including KAI1, KISS1, SERPINB5, TNFRSF25, TNFRSF1A, TNFRSF6 and SERPINB2, were significantly lower in eutopic endometrial tissue from patients with endometriosis than those from endometriosis-free women. The expressions of oncogenes (ERBB2, ETS2, FOS), apoptosis gene (BCL2L1), cyclin-dependent kinases (CDK4, CDC25A), and growth factor and receptors (HGF, FGFR2, TGFBR) were significantly enhanced, while the expressions of the anti-oncogenes (KAI1, SERPINB5), apoptosis genes (BAD and TNF) and cyclin-dependent kinase inhibiting factor (CDKN2A) were obviously reduced in ectopic tissue as compared with those in eutopic tissue from patients with endometriosis. Puerarin significantly enhanced the gene expressions in endometriotic stromal cells, including BAD, BAX, CASP8, CASP9, TNFRSF6, CDKN1B, CDKN2A, IFNA1 and IFNB1, and reduced the gene expressions of FOS, CHEK2, SRC, ITGB5, MMP9, PDGFA and NFKBIA.

Conclusions: The tumor-related gene expression has significant differences in eutopic endometrial tissue between patients with endometriosis and endometriosis-free women, and between ectopic and eutopic tissues from patients with endometriosis. Puerarin can reduce angiopoiesis, regulate tumor-related gene expression and facilitate apoptosis in endometriotic tissue.

Welcome to JCIM! You are the number 12477 reader of this article!
Download Article:
[Full Text]      [PDF]      [Chinese]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Yu CQ, Yu J, Han J, Zhou QL, Shen W. Regulatory mechanism of malignant behavior of endometriosis mediated by puerarin. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2009; 7(1): 41-47.
1Utsunomiya H, Cheng YH, Lin Z, Reierstad S, Yin P, Attar E, Xue Q, Imir G, Thung S, Trukhacheva E, Suzuki T, Sasano H, Kim JJ, Yaegashi N, Bulun SE. Upstream stimulatory factor-2 regulates steroidogenic factor-1 expression in endometriosis[J].Mol Endocrinol, 2008, 22(4): 904-914.  .
2Xue Q, Lin Z, Yin P, Milad MP, Cheng YH, Confino E, Reierstad S, Bulun SE. Transcriptional activation of steroidogenic factor-1 by hypomethylation of the 5'CpG island in endometriosis[J].J Clin Endocrinol Metab, 2007, 92(8): 3261-3267.  .
3Le Bail JC, Champavier Y, Chulia AJ, Habrioux G. Effects of phytoestrogens on aromatase, 3beta and 17beta-hydroxysteroid dehydrogenase activities and human breast cancer cells[J].Life Sci, 2000, 66(14): 1281-1291.  .
4Whitehead SA, Lacey M. Phytoestrogens inhibit aromatase but not 17β-hydroxysteroid dehydrogenase (HSD) type 1 in human granulosa-luteal cells: evidence for FSH induction of 17β-HSD[J].Human Reproduction, 2003, 18(3): 487-494.  .
5Shi SF, Yu CQ, Liu YH, Cui Y, Hui N. Primary culture of human eutopic and ectopic endometrial glandular epithelial cells and stromal cells[J].Di Er Jun Yi Da Xue Xue Bao, 2004, 25(11): 1228-1231. Chinese with abstract in English.
6Peeters LLH, Vigne JL, Tee MK, Zhao D, Wsite LL, Taylor RN. PPARgamma represses VEGF expression in human endometrial cells: Implications for uterine angiogenesis[J].Angiogenesis, 2006, 8(4): 373-379.  .
7Yu CQ, Cai ZL, Liu YH, Pan RP, Li J. Effect of Neiyi Recipe on cytokines and VEGF mRNA expression levels in endometriotic rats[J].Anhui Zhong Yi Xue Yuan Xue Bao, 2002, 21(6): 36-39. Chinese with abstract in English.
8Koninckx PR, Barlow D, Kennedy S. Implantation versus infiltration: the Sampson versus the endometriotic disease theory[J].Gynecol Obstet Invest, 1999, 47(Supp 1): 3-9.  .
9Taylor HS. Endometrial cells derived from donor stem cells in bone marrow transplant recipients[J].JAMA, 2004, 292(1): 81-85.  .
10Yu CQ, Shi SF, Wang RX, Liu YH, Song YH, Yu J. Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis[J]. J Chin Integr Med, 2006, 4(2): 189-193. Chinese with abstract in English.
11Hearns-Stokes R, Mayers C, Zahn C, Cruess D, Gustafsson JA, Segars J, Nieman L. Expression of the proto-oncoprotein breast cancer nuclear receptor auxiliary factor (Brx) is altered in eutopic endometrium of women with endometriosis[J].Fertil Steril, 2006, 85(1): 63-70.  .
12Khan KN, Masuzaki H, Fujishita A, Kitajima M, Hiraki K, Sekine I, Matsuyama T, Ishimaru T. Interleukin-6- and tumour necrosis factor alpha-mediated expression of hepatocyte growth factor by stromal cells and its involvement in the growth of endometriosis[J].Hum Reprod, 2005, 20(10): 2715-2723.  .
13Li YW, Cai ZL, Shen W, Yang SS, Yu CQ. Regulation of aromatase P450 expression by puerarin in endometrial cell line RL95-2[J]. J Chin Integr Med, 2008, 6(10): 1017-1023. English with abstract in Chinese.

 Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese
Copyright © 2003-2012 by JCIM Press. All rights reserved. ISSN 1672-1977