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Journal of Chinese Integrative Medicine: 2009; 7(7): 636-641
DOI: 10.3736/jcim20090707
Effects of genistein on interleukin-1β and tumor necrosis factor-α secreted by fibroblast-like synoviocytes isolated from type Ⅱ collagen-induced arthritis rats
1. Bo GAO (Department of Hematopathy and Rheumatism, Clinical Medicine College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China )
2. Yu ZHANG (Department of Hematopathy and Rheumatism, Clinical Medicine College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China E-mail: yzzy10182001@yahoo.com.cn)
3. Li MA (Department of Hematopathy and Rheumatism, Clinical Medicine College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China )
4. Xue-zeng ZHANG (Department of Hematopathy and Rheumatism, Clinical Medicine College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China )
Objective: To investigate the effects of genistein (Gen) on interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) secreted by fibroblast-like synoviocytes (FLSs) of rats with type Ⅱ collagen-induced arthritis (CIA).
Methods: Type Ⅱ collagen was injected to induce arthritis in rats, and arthritis index was applied to evaluate whether the arthritis was induced successfully. The primary FLSs were separated from synovial membranes by using type Ⅱ collagenase digestion. Then the expression of vascular cell adhesion molecule-1 (VCAM-1) was estimated by flow cytometry (FCM). After addition of different concentrations of Gen into the FLSs, IL-1β and TNF-α contents in the supernatants were measured by enzyme-linked immunosorbent assay.
Results: Three days after collagen injection, the rats started to develop arthrocele and the arthritis index increased gradually. The arthritis index of CIA group was significantly higher than that of the control group. The expression of VCAM-1 was up to 85.5% in the 4th generation FLSs, indicating that most part of the cultured cells were type-B synoviocytes. After administration of different concentrations of Gen (100, 200, 400 μmol/L), the contents of IL-1β and TNF-α in supernatants of FLSs were decreased dose-dependently.
Conclusion: Genistein can suppress the secretion of TNF-α and IL-1β in FLSs dose-dependently, which may be one of the mechanisms for genistein in inhibiting the arthromeningitis of CIA rats.
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Please cite this article as:
Gao B, Zhang Y, Ma L, Zhang XZ. Effects of genistein on interleukin-1β and tumor necrosis factor-α secreted by fibroblast-like synoviocytes isolated from type Ⅱ collagen-induced arthritis rats. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2009; 7(7): 636-641.
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