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Journal of Chinese Integrative Medicine: 2010; 8(1): 49-55
DOI: 10.3736/jcim20100110
Taohe Chengqi Tang ameliorates acute liver injury induced by carbon tetrachloride in rats
1. Tung-yuan LAI (School of PostBaccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan, China )
2. Yi-jiun WENG (Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan, China )
3. Wei-wen KUO (Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, China )
4. Li-mien CHEN (Department of Internal Medicine, Armed Forces Taichung General Hospital, Taichung 411, Taiwan, China )
5. Yun-ting CHUNG (Emergency Department, China Medical University Hospital, Taichung 404, Taiwan, China )
6. Yueh-min LIN (Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan, China )
7. Fuu-jen TSAI (Department of Pediatrics, Medical Research and Medical Genetics, China Medical University Hospital, Taichung 404, Taiwan, China )
8. Chun-hsin LEE (Center for Cardiovascular Diseases of Traditional Chinese Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China )
9. Yee-mun CHOONG (Graduate Institute of Chinese Medical Science, China Medical University, Taichung 404, Taiwan, China )
10. Eric Y.C. LAI (HopeSun Cancer Center, Taichung Hospital, Taichung 403, Taiwan, China )
11. Chih-yang HUANG (Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan, China )
12. Yu-lan YEH (Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan, China E-mail: cyhuang@mail.cmu.edu.tw)
Objective: To clarify the efficacy of Taohe Chengqi Tang (THCQT), a compound traditional Chinese herbal medicine, in protecting liver damage induced by carbon tetrachloride (CCl4) in rats.
Methods: Thirty male Wistar rats were divided into normal control group, untreated group, low-dose THCQT group (receiving 0.3 g/kg of THCQT), high-dose THCQT group (receiving 0.5 g/kg of THCQT), and positive control group (receiving silymarin 25 mg/kg). All testing substances were orally administered 1 hour before the intraperitoneal injection of CCl4 (1.5 mL/kg). Twenty-four hours after CCl4 injection, the rats were sacrificed to observe liver histopathological changes, and to evaluate activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and lipid peroxidation (LPO) and glutathione (GSH) levels in liver tissues.
Results: CCl4 injection elevated the serum AST and ALT activities, but THCQT significantly reversed this effect. The increase of hepatic LPO by CCl4 was markedly reduced by THCQT. Also, this herbal mixture increased hepatic GSH in the rats. In histopathology analysis, THCQT decreased the fatty accumulation, necrosis and lymphocyte infiltration. The in vitro study in rat brain showed that LPO induced by Fe2+/ascorbic acid was dose-dependently reduced by THCQT. According to the biochemical and morphological data, THCQT could protect the liver from CCl4-induced injuries.
Conclusion: THCQT seems helpful for protection of liver damage induced by chemicals depending on its anti-oxidant-like function, and THCQT is more effective than silymarin.
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Please cite this article as:
Lai TY, Weng YJ, Kuo WW, Chen LM, Chung YT, Lin YM, Tsai FJ, Lee CH, Choong YM, Lai EYC, Huang CY, Yeh YL. Taohe Chengqi Tang ameliorates acute liver injury induced by carbon tetrachloride in rats. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2010; 8(1): 49-55.
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