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Journal of Chinese Integrative Medicine: 2010; 8(1): 61-66
DOI: 10.3736/jcim20100112
Effects of cycloartocarpin A and artocarpin extracted from Fructus Artocarpi Heterophylli on apoptosis of SMMC-7721 and SGC-7901 cells
1. Yan-long YANG (Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
2. Ai-jun HOU (Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China )
3. Hui-qing ZHANG (Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
4. Hong SHEN (Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China )
5. Qiu-shuang LI (Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
6. Cong-cong ZHANG (Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
7. Guo-fu ZHU (Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
Objective: To investigate the effects of cycloartocarpin A (ACR-2) and artocarpin (ACR-3), monomeric compounds isolated from Fructus Artocarpi Heterophylli, on apoptosis of SMMC-7721 and SGC-7901 cell lines.
Methods: SMMC-7721 and SGC-7901 cells were routinely cultured, and divided into experiment group and control group. The SMMC-7721 cells were treated with different concentrations of ACR-2 (3.46×10-3, 13.82×10-3, 55.30×10-3 mmol/L) and ACR-3 (6.88×10-3, 27.52×10-3, 110.09×10-3 mmol/L), and the SGC-7901 cells were also treated with different concentrations of ACR-2 (8.06×10-3, 32.26×10-3, 129.03×10-3 mmol/L) and ACR-3 (2.87×10-3, 11.47×10-3, 45.87×10-3 mmol/L), with PBS (DMSO<0.1%) as control treatment. Cell apoptosis was measured by double labeled staining with Hoechst33342/propidium iodide (PI) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry.
Results: ACR-2 and ACR-3 could induce apoptosis of SMMC-7721 and SGC-7901 cells. Some of SMMC-7721 and SGC-7901 cells demonstrated typical apoptosis after being treated with ACR-2 and ACR-3. Hoechst33342/PI staining showed that cells were fraught with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared light blue. TUNEL showed that cells permeated with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared brown. Less apoptotic cells were observed in negative control group, and the nuclear appeared light blue. The apoptosis rates of SMMC-7721 and SGC-7901 cells in the ACR-2 and ACR-3 treated groups were significant higher than those in the control group (P<0.05, P<0.01).
Conclusion: ACR-2 and ACR-3 can induce apoptosis of SMMC-7721 and SGC-7901 cells.
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Please cite this article as:
Yang YL, Hou AJ, Zhang HQ, Shen H, Li QS, Zhang CC, Zhu GF. Effects of cycloartocarpin A and artocarpin extracted from Fructus. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2010; 8(1): 6166.
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