Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese Updated Sunday, August 02, 2015
 Services
Submit a manuscript
Subscribe
Individuals
Institutions
Response
Send response
Read responses
Reprint
Advance online publication
Sign up for e-alert
JCIM job opportunities
Contact JCIM
 Information For
Readers
Authors
Reviewers
Advertisers
 About JCIM
Journal description
Editorial policy
Privacy policy
Copy right
Editorial staff
 Language Polishing
Journal of Chinese Integrative Medicine: 2010; 8(7): 670-677
DOI: 10.3736/jcim20100711
Effects of acupuncture on the number and degranulation ratio of mast cells and expression of tryptase in synovium of rats with adjuvant arthritis
1. Tian-feng He (Laboratory Center of Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China )
2. Shu-hui Zhang (Department of Pathology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China )
3. Lian-bo Li (Laboratory Center of Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China )
4. Wen-jia Yang (Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China )
5. Jun Zhu (Laboratory Center of Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China )
6. Yun-fei Chen (Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China E-mail: icyf1968@yahoo.com.cn)
Objective: To observe the effects of acupuncture on synovial pathology, synovial mast cell degranulation and tryptase expression and to investigate the relationship between the functions of mast cells and effects of acupuncture on early adjuvant arthritis in rats.
Methods: Forty-six male Wistar rats were randomly divided into normal control group (n=16), untreated group (n=15) and acupuncture group (n=15). Adjuvant arthritis was induced by injection of 0.1 mL Freund’s complete adjuvant in right hind limb footpad. Normal control group and untreated group received no acupuncture treatment, while rats in the acupuncture group were treated with sterilized disposable stainless steel needles inserted perpendicularly as deep as 2 to 3 mm at Xuanzhong (GB39), 6 mm at Shenshu (BL23) and 7 mm at Zusanli (ST36) for eight times (15 min each time) every two days. Setting the modeling day as the 0 day of the experiment, the body weight and paw volume of the rats were measured every three days from the 0 day. In the end, synovial tissues of the right hind ankles were sampled and made into paraffin sections. Then they were firstly stained with hematoxylin-eosin for observing synovial pathology to evaluate the effects of acupuncture on adjuvant arthritis, then stained with toluidine blue for observing the number and degranulation ratio of synovial mast cells and finally detected by immunohistochemical staining method to investigate the expression of tryptase in synovium.
Results: Compared with the untreated group, the body weight of rats in the acpuncture group was increased significantly (P<0.05), while the paw volume decreased obviously (P<0.01). Hematoxylin-eosin staining showed that acupuncture significantly inhibited inflammatory cell infiltration, synovial cell hyperplasia, and synovial fibroplasia in synovium of rats with adjuvant arthritis as compared with the untreated group (P<0.05). Toluidine blue staining showed that acupuncture could significantly diminish the numbers of total and degranulated mast cells in rats with adjuvant arthritis (P<0.01), which were significantly higher in the untreated group than in the normal control group (P<0.01). Showing by immunohistochemical staining, the expression of tryptase in synovium in the acupuncture group was decreased as compared with the untreated group (P<0.01). Analyzed by Spearman’s bivariate correlation, the number of mast cells and degranulation ratio of mast cells were positively correlated with the pathological scores (r=0.837, P<0.01; r=0.634, P<0.01).
Conclusion: Acupuncture can improve pathological condition of inflammatory synovium in rats with early adjuvant arthritis by inhibiting the function of synovial mast cells, which may play an important underlying role in the immunoregulation of acupuncture on adjuvant arthritis. 
Welcome to JCIM! You are the number 8499 reader of this article!
Download Article:
[Full Text]      [PDF]      [Chinese]      [Previous]      [Next]      [This Issue]
Please cite this article as:
He TF, Zhang SH, Li LB, Yang WJ, Zhu J, Chen YF. Effects of acupuncture on the number and degranulation ratio of mast cells and expression of tryptase in synovium of rats with adjuvant arthritis. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2010; 8(7): 670-677.
References:
1Lee MS, Shin BC, Ernst E. Acupuncture for rheumatoid arthritis: a systematic review[J].Rheumatology (Oxford), 2008, 47(12): 1747-1753.  .
2Wang C, de Pablo P, Chen X, Schmid C, McAlindon T. Acupuncture for pain relief in patients with rheumatoid arthritis: a systematic review[J].Arthritis Rheum, 2008, 59(9): 1249-1256.  .
3Zijlstra FJ, van den Berg-de Lange I, Huygen FJ, Klein J. Anti-inflammatory actions of acupuncture[J].Mediators Inflamm, 2003, 12(2): 59-69.  .
4Cheng JJ, Cai NG, Zhai DG. The regulatory role of acupuncture and moxibustion on immune cells and molecules[J].Jiangsu Zhong Yi Yao, 2007, 39(11): 87-89. Chinese.
5Li SG, Zhao JP, Yue YE, Zhang PS, Su QS. The alteration of immune function among rheumatoid arthritis patients with warming acupuncture[J].Zhongguo Kang Fu Yi Xue Za Zhi, 1994, 9(5): 212-214. Chinese with abstract in English.
6Sun LQ, Liu XL, Xiao J, Yin SY. Clinical observation of acupuncture in treatment of 434 patients with rheumatoid arthritis[J].Jun Yi Jin Xiu Xue Yuan Xue Bao, 1992, 13(3): 259-261. Chinese.
7Wu F, Ji QS, Wang Q, Li J. Clinical observation of acupuncture in treatment of 105 patients with rheumatoid arthritis[J].Liaoning Zhong Yi Za Zhi, 1990, 14(9): 40-42. Chinese.
8Yim YK, Lee H, Hong KE, Kim YI, Lee BR, Son CG, Kim JE. Electro-acupuncture at acupoint ST36 reduces inflammation and regulates immune activity in collagen-induced arthritic mice[J].Evid Based Complement Alternat Med, 2007, 4(1): 51-57.  .
9He JN, Luo MF. Progress in the study on the relationship between effects of acu-moxibustion and mast cells in acupoints[J].Zhen Ci Yan Jiu, 2007, 32(3): 214-216. Chinese with abstract in English.
10Lee DM, Friend DS, Gurish MF, Benoist C, Mathis D, Brenner MB. Mast cells: a cellular link between autoantibodies and inflammatory arthritis[J].Science, 2002, 297(5587): 1689-1692.  .
11He SH, He YS, Xie H. IgE-dependent histamine and tryptase release from human mast cells[J].Mian Yi Xue Za Zhi, 2004, 20(5): 353-356. Chinese with abstract in English.
12Xu SQ, Bao ZS, Wu ZM, Wang Y, Qi YX, Zhang JX. Changes in pathomorphology of synovial membrane tissues and levels of serum interleukin 4,interleukin 10 in adjuvant-induced arthritis rats following acupuncture treatment[J].Zhongguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu, 2008, 12(28): 5440-5443. Chinese with abstract in English.
13Li ZR. Experimental acupuncture science[M]. Beijing: China Press of Traditional Chinese Medicine, 2004. 327-329. Chinese.
14Shi XF, Xue L, Hu JD, Wang D, Wang J. Effect of Bingwu Gao on expression of platelet-derived growth factor in rats with rheumatoid arthritis[J].Zhong Yi Wai Zhi Za Zhi, 2007, 16(4): 5-7. Chinese.
15Wang YY, Cui XJ, Han L. The effect on the expression of NF-κB and bcl-2 in synovium of joint of sodium artesunate in treating adjuvant arthritis of rats[J].Zhongguo Yi Yuan Yao Xue Za Zhi, 2005, 25(11): 1003-1005. Chinese with abstract in English.
16Wang DT, Shen Y. Exploration on pathogenesis of rheumatoid arthritis from “deficiency of Zang-organs and collateral disease”[J].Tianjin Zhong Yi Yao, 2008, 25(6): 482-483. Chinese with abstract in English.
17Shin K, Gurish MF, Friend DS, Pemberton AD, Thornton EM, Miller HR, Lee DM. Lymphocyte-independent connective tissue mast cells populate murine synovium[J].Arthritis Rheum, 2006, 54(9): 2863-2871.  .
18Xu SY, Bian RL, Chen X. Methodology of pharmacological experiments[M]. Beijing: People’s Medical Publishing House, 2002. 920.. Chinese.
19Caughey GH. Mast cell tryptases and chymases in inflammation and host defense[J].Immunol Rev, 2007, 217: 141-154.  .
20Christy AL, Brown MA. The multitasking mast cell: positive and negative roles in the progression of autoimmunity[J].J Immunol, 2007, 179(5): 2673-2679.  .
21Gotis-Graham I, Smith MD, Parker A, McNeil HP. Synovial mast cell responses during clinical improvement in early rheumatoid arthritis[J].Ann Rheum Dis, 1998, 57(11): 664-671.  .
22Palmer HS, Kelso EB, Lockhart JC, Sommerhoff CP, Plevin R, Goh FG, Ferrell WR. Protease-activated receptor 2 mediates the proinflammatory effects of synovial mast cells[J].Arthritis Rheum, 2007, 56(11): 3532-3540.  .
23Zhang D, Ding GH, Shen XY, Yao W, Lin J. Study on the correlation between meridian acupoints and mast cells[J].Zhen Ci Yan Jiu, 2005, 30(2): 115-119. Chinese with abstract in English.
24Li M, Shi J, Liu XC, Wang LN, Zhang J, Li LL, Guan XM. Effects of electroacupuncture on the number of subcutaneous mast cells in and beside the acupoint and the inflammatory pain focus in the rat[J].Zhongguo Zhen Jiu, 2003, 23(10): 597-601. Chinese with abstract in English.
25He JN, Luo MF, Guo Y, Li CH, Zhang JL. Correlation between the therapeutic efficacy of electroacupuncture of ear-body acupoints and mast cells in experimental gastric ulcer rats[J].Zhen Ci Yan Jiu, 2008, 33(3): 159-163. Chinese with abstract in English.
26Tai HQ, Jiang WF. Experimental study of acupuncture at Xiaguan (ST7) on rats with allergic rhinitis[J].Zhejiang Zhong Xi Yi Jie He Za Zhi, 2008, 18(12): 747-748. Chinese.
27Wu HG, Jiang B, Zhou EH, Shi Z, Shi DR, Cui YH, Kou ST, Liu HR. Regulatory mechanism of electroacupuncture in irritable bowel syndrome: preventing MC activation and decreasing SP VIP secretion[J].Dig Dis Sci, 2008, 53(6): 1644-1651.  .
28Liu LY, Peng A, Xing XY. A study on the action of mast cells involving the mechanism of the acupuncture-pilomoter-line[J].Beijing Shi Fan Da Xue Xue Bao Zi Ran Ke Xue Ban, 2005, 41(5): 522-525. Chinese with abstract in Englis.
29Nakanishi M, Furuno T. Molecular basis of neuroimmune interaction in an in vitro coculture approach[J].Cell Mol Immunol, 2008, 5(4): 249-259.  .

 Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese
Copyright © 2003-2012 by JCIM Press. All rights reserved. ISSN 1672-1977