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Journal of Chinese Integrative Medicine: 2011; 9(10): 1138-1144
DOI: 10.3736/jcim20111016
Effects of Chinese herbal medicine Yiqi Huayu Bushen Recipe on expressions of aggrecan and type Ⅹ collagen mRNAs in cells from degenerated human intervertebral discs
1. Ju-bao Li (School of Clinical Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine )
2. Hong-yun Zhao (Department of Orthopedics and Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine )
3. Yong-jian Zhao (Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine )
4. Quan Zhou (Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine )
5. Ning Li (School of Clinical Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine )
6. Peng Xu (Department of Orthopedics and Traumatology, Changzheng Hospital, Second Military Medical University )
7. Qi Shi (Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine )
8. Yong-jun Wang (Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine E-mail: yjwang88@hotmail.com)
Objective: To investigate the effects of serum containing Yiqi Huayu Bushen Recipe, a compound Chinese herbal medicine, on expressions of aggrecan and type Ⅹ collagen mRNAs of the degenerated intervertebral disc cells in patients with cervical spondylotic myelopathy.Methods: Six inpatients diagnosed as cervical spondylotic myelopathy and undergoing cervical decompression and fusion surgery to remove the degenerated disc tissue were enrolled in this study. Primary intervertebral disc cells were isolated using small tissue mass method. Serum containing Yiqi Huayu Bushen recipe were obtained after SD rats were fed with Yiqi Huayu Bushen recipe for 3 days. MTT method was used to detect proliferation of the first-passage cells isolated from degenerated intervertebral discs and growth curve was drawn. In the following tests, the first-passage cells were cultured with the sera containing drugs and sodium chloride (NaCl), respectively. Proliferation of intervertebral disc cells was detected by MTT method and expressions of aggrecan and type Ⅹ collagen mRNAs were detected by real-time polymerase chain reaction.Results: The growth curve of the first-passage cells of the degenerated intervertebral discs showed that the time of cell proliferation peak was the sixth day. Compared with the same concentrations of NaCl solution, serum containing Yiqi Huayu Bushen Recipe at 5%, 10% and 20% promoted cell proliferation and expression of aggrecan mRNA and decreased expression of type Ⅹ collagen mRNA (P<0.01). 10% serum containing Yiqi Huayu Bushen recipe was more effective than 5% and 20%.Conclusion: Yiqi Huayu Bushen Recipe inhibits degeneration of the intervertebral disc cells by regulating collagen and proteoglycan expressions.
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Please cite this article as:
Li JB, Zhao HY, Zhao YJ, Zhou Q, Li N, Xu P, Shi Q, Wang YJ. Effects of Chinese herbal medicine Yiqi Huayu Bushen Recipe on expressions of aggrecan and type Ⅹ collagen mRNAs in cells from degenerated human intervertebral discs. J Chin Integr Med. 2011; 9(10): 1138-1144.
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