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Journal of Chinese Integrative Medicine: 2012; 10(7): 777-783
DOI: 10.3736/jcim20120708
Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone
1. Xin-ying Feng (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China )
2. Min Zhu (Institute of Neurology, Fudan University, Shanghai 200040, China )
3. Qi-qi Zhang (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China )
4. Yi-ping Chen (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China )
5. Wen-wei Li (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China E-mail: E-mail: wenweili2000@yahoo.com.cn)
OBJECTIVE: To observe the protective effects of echinacoside on rotenone-induced damages in rats.
METHODS: Healthy male Sprague-Dawley rats, weighing from 200 to 220 g, were randomly divided into five groups with 20 rats in each group: control group, rotenone group and echinacoside groups of low, medium and high doses (20, 40 and 80 mg/(kg·d)). Rats in the rotenone group were injected intraperitoneally for four weeks with rotenone (2.75 mg/(kg·d)), dissolved into dimethyl sulfoxide; rats in the control group were injected intraperitoneally with dimethyl sulfoxide daily, and rats in the echinacoside groups received daily intraperitoneal injection of rotenone along with echinacoside gastric perfusion for four weeks. Modified neurological severity score was used to evaluate neurobehavior of the animals; dopaminergic neurons in substantia nigra were observed by immunochemical method and dopamine concentration in striatum was determined by a fluorescence spectrophotometer. Biomarkers of liver and kidney damage were also measured.
RESULTS: In the rotenone group, the rats suffered from severe neurological disability (P<0.01), and the number of dopaminergic neurons in substantia nigra and dopamine concentration in striatum were decreased (P<0.05) compared with the normal control group; levels of the biomarkers for evaluating liver and kidney damage were increased (P<0.05). In the echinacoside groups, the neurological disability and the loss of dopaminergic neurons in substantia nigra were suppressed and dopamine concentrations in striatum were increased (P<0.05), but the liver and kidney damage was not improved (P>0.05).
CONCLUSION: Rotenone causes severe damages to dopaminergic neurons, liver and kidney in rats and echinacoside selectively reverses dopaminergic neuronal injury.
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Please cite this article as:
Feng XY, Zhu M, Zhang QQ, Chen YP, Li WW. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(7): 777-783.
References:
1Zhao X, Pu XP, Geng XC. Effects of echinacoside on protein expression from substantia nigra and striatal tissue in mouse MPTP model of Parkinsons disease by using 2-dimensional electrophoresis analysis[J].Zhongguo Yao Li Xue Tong Bao, 2008, 24(1): 28-32. Chinese with abstract in English.
2Zhao Q, Gao J, Li W, Cai D. Neurotrophic and neurorescue effects of echinacoside in the subacute MPTP mouse model of Parkinson’s disease[J].Brain Res, 2010, 1346: 224-236.  .
3Dauer W, Przedborski S. Parkinson’s disease: mechanisms and models[J].Neuron, 2003, 39(6): 889-909.  .
4Cannon JR, Tapias V, Na HM, Honick AS, Drolet RE, Greenamyre JT. A highly reproducible rotenone model of Parkinson’s disease[J].Neurobiol Dis, 2009, 34(2): 279-290.  .
5Lapointe N, St-Hilaire M, Martinoli MG, Blanchet J, Gould P, Rouillard C, Cicchetti F. Rotenone induces non-specific central nervous system and systemic toxicity[J].FASEB J, 2004, 18(6): 717-719.  .
6Chen Y, Constantini S, Trembovler V, Weinstock M, Shohami E. An experimental model of closed head injury in mice: pathophysiology, histopathology, and cognitive deficits[J].J Neurotrauma, 1996, 13(10): 557-568.  .
7Yao QH, Gao GD. Inosine is neuroprective against MPTP-induced Parkinson’s disease in C57BL mice[J].Di Si Jun Yi Da Xue Xue Bao, 2005, 26(2): 141-145. Chinese with abstract in English.
8Zhan QJ, Zhang YY, Huang WY. Traditional Chinese medicine in treatment of Parkinson’s disease[J]. J Chin Integr Med, 2004, 2(1): 75-77. Chinese with abstract in English.
9He WJ, Fang TH, Tu PF. Research progress on pharmacological activities of echinacoside[J].Zhongguo Zhong Yao Za Zhi, 2009, 34(4): 476-479. Chinese with abstract in English.
10An CN, Zhang HN, Pu XP. Research progress on neuropharmacological activities of Cistanche. Zhongguo Yao Xue Za Zhi. 2011; 46(12): 887-890. Chinese.
11Langston JW, Ballard P, Tetrud JW, Irwin I. Chronic Parkinsonism in humans due to a product of meperidine-analog synthesis[J].Science, 1983, 219(4587): 979-980.  .
12von Bohlen und Halbach O, Schober A, Krieglstein K. Genes, proteins, and neurotoxins involved in Parkinson’s disease[J].Prog Neurobiol, 2004, 73(3): 151-177.  .
13Heikkila RE, Manzino L, Cabbat FS, Duvoisin RC. Protection against the dopaminergic neurotoxicity of 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine by monoamine oxidase inhibitors[J].Nature, 1984, 311(5985): 467-469.  .
14Javitch JA, D’Amato RJ, Strittmatter SM, Snyder SH. Parkinsonism-inducing neurotoxin, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine: uptake of the metabolite N-methyl-4-phenylpyridine by dopamine neurons explains selective toxicity[J].Proc Natl Acad Sci U S A, 1985, 82(7): 2173-2177.  .
15Dick FD, De Palma G, Ahmadi A, Scott NW, Prescott GJ, Bennett J, Semple S, Dick S, Counsell C, Mozzoni P, Haites N, Wettinger SB, Mutti A, Otelea M, Seaton A, Soderkvist P, Felice A. Environmental risk factors for Parkinson’s disease and parkinsonism: the Geoparkinson study[J].Occup Environ Med, 2007, 64(10): 666-672.  .
16Greenamyre JT, Betarbet R, Sherer TB. The rotenone model of Parkinson’s disease: genes, environment and mitochondria[J].Parkinsonism Relat Disord, 2003, 9(Suppl 2): S59-S64.  .
17Xiong Q, Hase K, Tezuka Y, Namba T, Kadota S. Acteoside inhibits apoptosis in D-galactosamine and lipopolysaccharide-induced liver injury[J].Life Sci, 1999, 65(4): 421-430.  .
18Wu Y, Li L, Wen T, Li YQ. Protective effects of echinacoside on carbon tetrachloride-induced hepatotoxicity in rats[J].Toxicology, 2007, 232(1-2): 50-56.  .
19Wu Y, Xu GL, Lou M, Zheng Z. The protective effect of echinacoside on acute liver injury in rats[J].Wei Chang Bing Xue He Gan Bing Xue Za Zhi, 2008, 17(5): 410-412. Chinese with abstract in English.

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