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Journal of Chinese Integrative Medicine: 2012; 10(12): 1427-1432
DOI: 10.3736/jcim20121215
Effects of echinacoside on MPP+-induced mitochondrial fragmentation, mitophagy and cell apoptosis in SH-SY5Y cells
1. Min Zhu (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China )
2. Mi Zhou (Institute of Brain Science, Fudan University, Shanghai 200032, China )
3. Ying Shi (Institute of Brain Science, Fudan University, Shanghai 200032, China )
4. Wen-wei Li (Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China )
OBJECTIVE: To observe the protective effect of echinacoside on mitochondrial fragmentation, mitophagy and cell apoptosis in human neuroblastoma SH-SY5Y cell line exposed to 1-methyl-4-phenylpyridinium (MPP+).
METHODS: The MPP+ -treated SH-SY5Y cells were divided into control and drug groups. The cell apoptosis was determined by flow cytometry analysis. The alterations in mitochondrial morphology were observed, and the changes in mitochondrial membrane potential and mitophagy were measured.
RESULTS: MPP+ induced severe mitochondrial fragmentation in SH-SY5Y cells. Mitochondrial membrane potential was significantly reduced (P<0.05), which further led to mitophagy and cell apoptosis in SH-SY5Y cells treated with MPP+ (P<0.05); mitochondrial depolarization, mitophagy and cell apoptosis in echinacoside-treated groups were significantly suppressed (P<0.05), but mitochondrial fragmentation was not affected (P>0.05).
CONCLUSION: MPP+ can cause severe damage to mitochondrial morphology and the function in SH-SY5Y cells, and echinacoside selectively reverse mitochondrial function and cell apoptosis, yet there is no effect on mitochondrial morphology.
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Please cite this article as:
Zhu M, Zhou M, Shi Y, Li WW. Effects of echinacoside on MPP+-induced mitochondrial fragmentation, mitophagy and cell apoptosis in SH-SY5Y cells. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 15(12): 1427-1432.
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