Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese Updated Wednesday, September 03, 2014
 Services
Submit a manuscript
Subscribe
Individuals
Institutions
Response
Send response
Read responses
Reprint
Advance online publication
Sign up for e-alert
JCIM job opportunities
Contact JCIM
 Information For
Readers
Authors
Reviewers
Advertisers
 About JCIM
Journal description
Editorial policy
Privacy policy
Copy right
Editorial staff
 Language Polishing
Journal of Chinese Integrative Medicine: 2013; 11(4): 253-261
DOI: 10.3736/jintegrmed2013025
Pharmacological evaluation of Musa seminifera Lour. fruit
1. Sanjib Saha (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh E-mail: sanjibsaha1991@yahoo.com)
2. Faroque Hossain (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
3. Md. Anisuzzman (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
4. Md. Khirul Islam (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )

OBJECTIVE: To study potential antioxidant, analgesic, antidiarrheal, and antibacterial activities of the ethanol extract of Musa seminifera Lour. fruit in different established in vivo and in vitro experimental models.

METHODS: In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was determined using Folin-Ciocalteu’s reagent. Reducing ability was evaluated by ferric reducing power assay. Peripherally and centrally acting analgesic activity was studied in three different in vivo models, namely, acetic acid-induced writhing, hot-plate test, and tail-flick test in Swiss albino mice. In vivo antidiarrheal activity was evaluated in castor oil- and magnesium sulfate-induced diarrhea in mice. Gastrointestinal motility test was also carried out in mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was assessed by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Acute toxicity test was conducted to assess the safe doses of the extract.

RESULTS: The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radical-scavenging assay. Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g of dried fruits extract. Reducing power was in a concentration-dependent manner, and strongly comparable with the standard ascorbic acid. The extract demonstrated significant inhibition of writhing in acetic acid-induced writhing test at both dose levels (P<0.01). The extract also raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner, and the results were statistically significant (P<0.01). The extract significantly (P<0.01) increased latent period, and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also decreased gastrointestinal motility in mice. In disk diffusion assay, the extract showed potential antibacterial activity against all the tested bacterial strains.

CONCLUSION: The results suggest that the ethanol extract of M. seminifera fruit has potential antioxidant, analgesic, antidiarrheal, and antibacterial activities.

Welcome to JCIM! You are the number 14045 reader of this article!
Download Article:
[Full Text]      [PDF]      [Chinese]      [Next]      [This Issue]
Please cite this article as:
Saha S, Hossain F, Anisuzzman M, Islam MK. Pharmacological evaluation of Musa seminifera Lour. fruit. J Integr Med. 2013; 11(4): 253-261.
References:
1Samuelson AD. Medicinal values of plantain. 3rd ed[M]. Oxford and Edinburgh: Blackwell Scientific Publication, 1980. 30-37.  .
2Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, Roodenrys S, Keogh JB, Clifton PM, Williams PG, Fazio VA, Inge KE. Health benefits of herbs and spices: The past, the present, the future[J].Med J Aust, 2006, 185(4 Suppl): S4-S24.  .
3Partha P, Hossain ABME. Ethanobotanical investigation into the Mandi ethnic community in Bangladesh[J].Bangladesh J Plant Taxon, 2007, 14(2): 129-145.  .
4Ghani A. Medicinal plants of Bangladesh: chemical constituents and uses. 2nd ed[M]. Dhaka: The Asiatic Society of Bangladesh, 2003. 315.  .
5Khare CP. Indian medicinal plants: An illustrated dictionary[M]. New York: Springer Science+Business Media, LLC, 2007. 426.  .
6Goel RK, Sairam K. Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiber officinale[J].Indian J Pharmacol, 2002, 34(2): 100-110.  .
7Zafar IM, Saleha A, Hoque MME, Sohel RM. Antimicrobial and cytotoxic properties of different extracts of Musa sapientum L. subsp. sylvestris[J].Indian J Exp Biol, 2011, 2(8): 62-65.  .
8Agarwal PK, Singh A, Gaurav K, Goel S, Khanna HD, Goel RK. Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats[J].Indian J Exp Biol, 2009, 47: 322-340.  .
9Tewtrakul S, Itharat A, Thammaratwasik P, Ooraikul B. Anti-allergic and anti-microbial activities of some Thai crops[J].Songklanakarin J Sci Technol, 2008, 30(4): 467-473.  .
10Mallick C, Chatterjee K, Guhabiswas M, Ghosh D. Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in streptozotocin-induced diabetic male albino rat[J].Afr J Tradit Complement Altern Med, 2007, 4(3): 362-371.  .
11Waalkes TP, Sjoerdsma A, Creveling CR, Weissbach H, Udenfriend S. Serotonin, norepinephrine, and related compounds in Bananas[J].Science, 1958, 127(3299): 648-650.  .
12Shanmugavelu KG, Rangaswami G. Tryptophan and indole compounds in banana ovaries[J].Nature, 1962, 194: 775-776.  .
13Lewis DA, Shaw GP. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions[J].J Nutr Biochem, 2001, 12(2): 95-100.  .
14Lewis DA, Fields WN, Shaw GP. A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions[J].J Ethnopharmacol, 1999, 65(3): 283-288.  .
15Ghoshal S. Steryl glycosides and acyl steryl glycosides from Musa paradisiaca[J].Phytochemistry, 1985, 24(8): 1807-1810.  .
16Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities of banana (Musa, AAA cv. Cavendish) fruits peel[J].Am J Biochem Biotechnol, 2005, 1(3): 125-131.  .
17Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities[J].Chem Pharm Bull, 2003, 51(5): 595-598.  .
18Sharma OP, Bhat TK. DPPH antioxidant assay revisited[J].Food Chem, 2009, 113(4): 1202-1205.  .
19Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables[J].J Univ Chem Technol Metallurgy, 2005, 40(3): 255-260.  .
20Talukder C, Saha S, Adhikari S, Mondal HK, Islam MK, Anisuzzman M. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Flacourtia jangomas (Lour.) Raeusch. leaves[J].Pharmacologyonline, 2012, 3: 20-28.  .
21Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine[J].Jpn J Nutr, 1986, 44: 307-315.  .
22Lorke D. A new approach to practical acute toxicity testing[J].Arch Toxicol, 1983, 54(4): 275-287.  .
23Koster R, Anderson M, De Beer EJ. Acetic acid for analgesics screening[J].Fed Proc, 1959, 18: 412-417.  .
24Saha S, Islam MK, Anisuzzman M, Hasan MM, Hossain F, Talukder C. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Phoenix paludosa Roxb. leaves[J].Int J Basic Med Sci Pharm, 2012, 2: 46-52.  .
25Karmakar UK, Sultana R, Biswas NN. Antioxidant, analgesic and cytotoxic activities of Mimusops elengi Linn. leaves[J].Int J Pharm Sci Res, 2011, 2(11): 2791-2797.  .
26Eddy NB, Leimback D. Synthetic analgesics. II. Dithienyl-butenyl and dithienybutylamines[J].J Pharmacol Exp Ther, 1953, 107(3): 385-393.  .
27Aydin S, Demir T, Oztürk Y, Ba?er KH. Analgesic activity of Nepeta italica L[J].Phytother Res, 1999, 13(1): 20-23.  .
28Abdullahi AL, Agho MO, Amos S, Gamaniel KS, Wambebe C. Antidiarrhoeal activity of the aqueous extract of Terminalia avicennoides roots[J].Phytother Res, 2001, 15(5): 431-434.  .
29Doherty NS. Inhibition of arachidonic acid release as the mechanism by which glucocorticoids inhibit endotoxin-induced diarrhoea[J].Br J Pharmacol, 1981, 73(2): 549-554.  .
30Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method[J].Am J Clin Pathol, 1966, 45(4): 493-496.  .
31Saha S, Anisuzzman M, Islam MK, Mondal H, Talukder C. Antibacterial and cytotoxic potential of Dalbergia spinosa Roxb. leaves[J].Int J Pharm Sci Res, 2013, 4(1): 512-515.  .
32Paix?o N, Perestrelo R, Marques JC, Camara JS. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines[J].Food Chem, 2007, 105(1): 204-214.  .
33Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale)[J].Food Chem, 2008, 111(1): 38-44.  .
34Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions[J].Food Chem, 2003, 83(4): 547-550.  .
35Li HY, Hao ZB, Wang XL, Huang L, Li JP. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance[J].Bioresour Technol, 2009, 100(2): 970-974.  .
36Ribeiro SMR, Barbosa LCA, Queiroz J, Knodler HM, Schieber A. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties[J].Food Chem, 2008, 110(3): 620-626.  .
37Headley PM, O’Shaughnessy CT. Evidence for opiate and dopamine interaction in striatum[J].Br J Pharmacol, 1985, 86: 700.  .
38Wigdor S, Wilcox GL. Central and systemic morphine-induced antinociception in mice: Contribution of descending serotonergic and noradrenergic pathways[J].J Pharmacol Exp Ther, 1987, 242(1): 90-95.  .
39Pierce NF, Carpenter CC Jr, Elliott HL, Greenough WB 3rd. Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum[J].Gastroenterology, 1971, 60(1): 22-32.  .
40Zavala MA, Pérez S, Pérez C, Vargas R, Pérez RM. Antidiarrhoeal activity of Waltheria americana, Commelina coelestis and Alternanthera repens[J].J Ethnopharmacol, 1998, 61(1): 41-47.  .
41Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition[J].Free Radic Res, 2006, 40(2): 223-231.  .

 Home | Current Issue | Past Issues | Search | CollectionsRSS | PDA Services | FAQ | SHCIM Online | Chinese
Copyright © 2003-2012 by JCIM Press. All rights reserved. ISSN 1672-1977