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Journal of Chinese Integrative Medicine: 2013; 11(5): 337-342
DOI: 10.3736/jintegrmed2013042
Effects of rutin on oxidative stress in mice with kainic acid-induced seizure
1. Marjan Nassiri-Asl (Cellular and Molecular Research Centre, Department of Pharmacology, Qazvin University of Medical Sciences, Qazvin, Iran E-mail: mnassiriasl@qums.ac.ir)
2. Taghi Naserpour Farivar (Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran )
3. Esmail Abbasi (Department of Pharmacology, Qazvin University of Medical Sciences, Qazvin, Iran )
4. Hamid Reza Sadeghnia (Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran )
5. Mehdi Sheikhi (School of Medicine, Tehran University of Medical Sciences, Tehran, Iran )
6. Mina Lotfizadeh (School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran )
7. Parisa Bazahang (School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran )

OBJECTIVE: Flavonoids are present in foods such as fruits and vegetables. Several studies have demonstrated a relationship between the consumption of flavonoid-rich foods and prevention of human disease, including neurodegenerative disorders. We assessed the effect of rutin (quercetin-3-O-rutinoside) on oxidative stress in kainic acid (KA)-induced seizure.

METHODS: Thirty-six BALB/c mice were randomly divided into three groups. In the control group, saline (intra-peritoneal, i.p.) was administered for 7 d, and on the last day, KA (10 mg/kg, i.p.) was injected 30 min after administration of saline. In rutin groups, mice were pretreated with rutin (100 and 200 mg/kg, i.p.) for 7 d, and on the last day, KA (10 mg/kg, i.p.) was injected 30 min after administration of rutin. Subsequently, behavioural changes were observed in mice. Lipid peroxidation and oxidative stress were measured respectively in the early and late phases after KA-induced seizures.

RESULTS: Seizure scores in the rutin groups were significantly lower than those in the control group (P < 0.01). Furthermore, rutin dose-dependently inhibited the number of wet-dog shakes (WDS) (P < 0.05). Malondialdehyde level in the hippocampus of the rutin groups was significantly lower than that in the hippocampus of the control group on days 1 and 21 after KA administration. In the rutin groups, the thiol levels observed on day 1 after KA administration were higher than that in the control group (P < 0.01).

CONCLUSION: These results indicate that rutin has potential anticonvulsant and antioxidative activities against oxidative stress in KA-induced seizure in mice.

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Please cite this article as:
Nassiri-Asl M, Naserpour Farivar T, Abbasi E, Sadeghnia HR, Sheikhi M, Lotfizadeh M, Bazahang P. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. J Integr Med. 2013; 11(5): 337-342.
References:
1Simon RP, Greenberg DA, Aminof MJ. Clinical neurology. 7th ed[M]. New York: McGraw-Hill Companies, Inc, 2009. 272.  .
2Gupta YK, Briyal S. Protective effect of vineatrol against kainic acid induced seizures, oxidative stress and on the expression of heat shock proteins in rats[J].Eur Neuropsycho-pharmacol, 2006, 16(2): 85-91.  .
3Yalcin A, Kilinc E, Kocturk S, Resmi H, Sozmen EY. Effect of melatonin cotreatment against kainic acid on coenzyme Q10, lipid peroxidation and Trx mRNA in rat hippocampus[J].Int J Neurosci, 2004, 114(9): 1085-1897.  .
4Shin HJ, Lee JY, Son E, Lee DH, Kim HJ, Kang SS, Cho GJ, Choi WS, Roh GS. Curcumin attenuates the kainic acid-induced hippocampal cell death in the mice[J].Neurosci Lett, 2007, 416(1): 49-54.  .
5Ben-Ari Y, Cossart R. Kainate, a double agent that generates seizures: two decades of progress[J].Trends Neurosci, 2000, 23(11): 580-587.  .
6Liang LP, Ho YS, Patel M. Mitochondrial superoxide production in kainate-induced hippocampal damage[J].Neuroscience, 2000, 101(3): 563-570.  .
7Won SJ, Ko HW, Kim EY, Park EC, Huh K, Jung NP, Choi I, Oh YK, Shin HC, Gwag BJ. Nuclear factor κB-mediated kainate neurotoxicity in the rat and hamster hippocampus[J].Neuroscience, 1999, 94(1): 83-91.  .
8Gupta YK, Briyal S, Chaudhary G. Protective effect of trans-resveratrol against kainic acid-induced seizures and oxidative stress in rats[J].Pharmacol Biochem Behav, 2002, 71(1-2): 245-249.  .
9Kuntic V, Pejic N, Ivkovic B, Vujic Z, Ilic K, Micic S, Vukojevic V. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms[J].J Pharm Biomed Anal, 2007, 43(2): 718-721.  .
10Marks SC, Mullen W, Borges G, Crozier A. Absorption, metabolism, and excretion of cider dihydrochalcones in healthy humans and subjects with an ileostomy[J].J Agric Food Chem, 2009, 57(5): 2009-2015.  .
11Trumbeckaite S, Bernatoniene J, Majiene D, Jakstas V, Savickas A, Toleikis A. The effect of flavonoids on rat heart mitochondrial function[J].Biomed Pharmacother, 2006, 60(5): 245-248.  .
12Pu F, Mishima K, Irie K, Motohashi K, Tanaka Y, Orito K, Egawa T, Kitamura Y, Egashira N, Iwasaki K, Fujiwara M. Neuroprotective effects of quercetin and rutin on spatial memory impairment in an 8-arm radial maze task and neuronal death induced by repeated cerebral ischemia in rats[J].J Pharmacol Sci, 2007, 104(4): 329-334.  .
13Richetti SK, Blank M, Capiotti KM, Piato AL, Bogo MR, Vianna MR, Bonan CD. Quercetin and rutin prevent scopolamine-induced memory impairment in zebrafish[J].Behav Brain Res, 2011, 217(1): 10-15.  .
14Javed H, Khan MM, Ahmad A, Vaibhav K, Ahmad ME, Khan A, Ashafaq M, Islam F, Siddiqui MS, Safhi MM, Islam F. Rutin prevents cognitive impairments by ameliorating oxidative stress and neuroinflammation in rat model of sporadic dementia of Alzheimer type[J].Neuroscience, 2012, 210: 340-352.  .
15Gupta R, Singh M, Sharma A. Neuroprotective effect of antioxidants on ischaemia and reperfusion-induced cerebral injury[J].Pharmacol Res, 2003, 48(2): 209-215.  .
16Khan MM, Ahmad A, Ishrat T, Khuwaja G, Srivastawa P, Khan MB, Raza SS, Javed H, Vaibhav K, Khan A, Islam F. Rutin protects the neural damage induced by transient focal ischemia in rats[J].Brain Res, 2009, 1292: 123-135.  .
17Rockhold RW, Jin C, Huang HM, Farley JM. Acute tachycardia and pressor effects following injections of kainic acid into the antero-dorsal medial hypothalamus[J].Neuropharmacology, 1987, 26(6): 567-573.  .
18Golechha M, Chaudhry U, Bhatia J, Saluja D, Arya DS. Naringin protects against kainic acid-induced status epilepticus in rats: evidence for an antioxidant, anti-inflammatory and neuroprotective intervention[J].Biol Pharm Bull, 2011, 34(3): 360-365.  .
19Morrison RS, Wenzel HJ, Kinoshita Y, Robbins CA, Donehower LA, Schwartzkroin PA. Loss of the p53 tumor suppressor gene protects neurons from kainate-induced cell death[J].J Neurosci, 1996, 16(4): 1337-1345.  .
20Shin EJ, Jeong JH, Kim AY, Koh YH, Nah SY, Kim WK, Ko KH, Kim HJ, Wie MB, Kwon YS, Yoneda Y, Kim HC. Protection against kainate neurotoxicity by ginsenosides: attenuation of convulsive behavior, mitochondrial dysfunction, and oxidative stress[J].J Neurosci Res, 2009, 87(3): 710-722.  .
21López-Meraz ML, González-Trujano ME, Neri-Bazán L, Hong E, Rocha LL. 5-HT1A receptor agonists modify epileptic seizures in three experimental models in rats[J].Neuropharmacology, 2005, 49(3): 367-375.  .
22Shin EJ, Ko KH, Kim WK, Chae JS, Yen TP, Kim HJ, Wie MB, Kim HC. Role of glutathione peroxidase in the ontogeny of hippocampal oxidative stress and kainate seizure sensitivity in the genetically epilepsy-prone rats[J].Neurochem Int, 2008, 52(6): 1134-1147.  .
23Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury[J].Free Radic Biol Med, 1990, 9(6): 515-540.  .
24Ellman GL. Tissue sulfhydryl groups[J].Arch Biochem Biophys, 1959, 82(1): 70-77.  .
25Hosseinzadeh H, Taiari S, Nassiri-Asl M. Effect of thymoquinone, a constituent of Nigella sativa L., on ischemia-reperfusion in rat skeletal muscle[J].Naunyn-Schmiedebergs Arch Pharmacol, 2012, 385(5): 503-508.  .
26Rodrigues MC, Rossetti F, Foresti ML, Arisi GM, Furtado MA, Dal-Cól ML, Bertti P, Fernandes A, Santos FL, Del Vecchio F, Garcia-Cairasco N. Correlation between shaking behaviors and seizure severity in five animal models of convulsive seizures[J].Epilepsy Behav, 2005, 6(3): 328-336.  .
27Shin C, Pedersen HB, McNamara JO. γ-Aminobutyric acid and benzodiazepine receptors in the kindling model of epilepsy: a quantitative radio histochemical study[J].J Neurosci, 1985, 5(10): 2696-2701.  .
28Nassiri-Asl M, Shariati-Rad S, Zamansoltani F. Anticonvulsive effects of intracerebroventricular administration of rutin in rats[J].Prog Neuropsychopharmacol Biol Psychiatry, 2008, 32(4): 989-993.  .
29Nassiri-Asl M, Mortazavi SR, Samiee-Rad F, Zangivand AA, Safdari F, Saroukhani S, Abbasi E. The effects of rutin on the development of pentylenetetrazole kindling and memory retrieval in rats[J].Epilepsy Behav, 2010, 18(1-2): 50-53.  .
30Marder M, Paladini AC. GABAA-receptor ligands of flavonoid structure[J].Curr Top Med Chem, 2002, 2(8): 853-867.  .
31Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, Paladini AC. Overview — flavonoids: a new family of benzodiazepine receptor ligands[J].Neurochem Res, 1997, 22(4): 419-425.  .
32Kim HC, Choi DY, Jhoo WK, Lee DW, Koo CH, Kim C. Aspalatone, a new antiplatelet agent, attenuates the neurotoxicity induced by kainic acid in the rat[J].Life Sci, 1997, 61(24): PL373-PL381.  .
33Shin EJ, Jeong JH, Chung YH, Kim WK, Ko KH, Bach JH, Hong JS, Yoneda Y, Kim HC. Role of oxidative stress in epileptic seizures[J].Neurochem Int, 2011, 59(2): 122-137.  .
34Dal-Pizzol F, Klamt F, Vianna MM, Schr?der N, Quevedo J, Benfato MS, Moreira JC, Walz R. Lipid peroxidation in hippocampus early and late after status epilepticus induced by pilocarpine or kainic acid in Wistar rats. Neurosci Lett 2000; 291(3): 179-182.
35Ueda Y, Yokoyama H, Nakajima A, Tokumaru J, Doi T, Mitsuyama Y. Glutamate excess and free radical formation during and following kainic acid-induced status epilepticus[J].Exp Brain Res, 2002, 147(2): 219-226.  .
36Dringen R. Metabolism and functions of glutathione in brain[J].Prog Neurobiol, 2000, 62(6): 649-671.  .
37Janáky R, Ogita K, Pasqualotto BA, Bains JS, Oja SS, Yoneda Y, Shaw CA. Glutathione and signal transduction in the mammalian CNS[J].J Neurochem, 1999, 73(3): 889-902.  .
38Aguiar CC, Almeida AB, Araújo PV, de Abreu RN, Chaves EM, do Vale OC, Macêdo DS, Woods DJ, Fonteles MM, Vasconcelos SM. Oxidative stress and epilepsy: literature review[J].Oxid Med Cell Longev, 2012, 2012: 795259.  .

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