Updated Tuesday, September 26, 2017
JCIM & JIM
Open Access
THIS ARTICLE
-  Abstract
-  Full text
-  Download PDF file
-  Send to a friend
-  Related articles in JCIM & JIM
-  Cited in JCIM & JIM
-  Reader's comments
-  Send a comment
 Language Polishing
Original Experimental Research
Journal of Chinese Integrative Medicine: Volume 9, 2011   Issue 7
Effects of Chinese herbal medicine Liuwei Dihuang Pill and its main monomer catalpol on transforming growth factor-β1/Smad pathway in HK-2 cells
1. Yu-min Liu (Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
2. Yue Zhang (Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China E-mail: yuezhang_shanghai@yahoo.com.cn)
3. Yan-peng Hao (Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
4. Zhi-jie Li (Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )

Objective: To observe the effects of sera containing Liuwei Dihuang Pill (LDP), a compound traditional Chinese herbal medicine, and its monomer catalpol on transforming growth factor-β1 (TGF-β1)/Smad pathway in HK-2 cells.
Methods: Wistar rats were intragastrically administered with LDP twice daily for 3 days and then sera were obtained. HK-2 cells were cultured at different concentrations of serum containing LDP. Lactate dehydrogenase (LDH) activity in the medium and cell activity, tested by methyl thiazolyl tetrazolium (MTT) method, were combined to determine the best concentration of the pill. Hepatocyte growth factor (HGF) was used as a positive control, while Western blotting was applied to observe the effects of sera containing LDP and catalpol on Smad2 phosphorylation and protein expressions of Smad2, Smad7 and SnoN.
Results: The 10% concentration of serum containing LDP was selected to carry out this study, as it showed no change of LDH activity in the medium and vitality of cells cultured with serum from normal rats. Similar to the mechanisms of HGF, the 10% concentration of serum containing LDP significantly inhibited the phosphorylation of Smad2 protein and up-regulated the expression of the Smad transcriptional co-repressor SnoN in TGF-β1-stimulated HK-2 cells. Catalpol inhibited phoshporylation of Smad2 without affecting SnoN protein expression. Neither serum containing LDP nor catalpol showed typical regulation effects on Smad7 expression in TGF-β1-stimulated HK-2 cells.
Conclusion: Serum containing LDP at 10% concentration has a potent antagonistic action on TGF-β1/Smad pathway in TGF-β1-stimulated HK-2 cells, which is similar to HGF. Catalpol is one of the most important monomers that plays a key role in LDP.

Received November 4, 2010; accepted April 12, 2011; published online July 15, 2011.
Full-text LinkOut at PubMed. Journal title in PubMed: Zhong Xi Yi Jie He Xue Bao.
    
Correspondence: Yue Zhang, MD, Professor; Tel: 021-51322391; E-mail: yuezhang_shanghai@yahoo.com.cn

 

Full text of this article is in Chinese

  
References
1. Jinde K, Nikolic-Paterson DJ, Huang XR, Sakai H, Kurokawa K, Atkins RC, Lan HY. Tubular phenotypic change in progressive tubulointerstitial fibrosis in human glomerulonephritis[J]. Am J Kidney Dis, 2001, 38(4) : 761-769.
    
2. Zhou J, Wang F, Lu HY, Zhang Y. Impact of salvianolic acid-B on TGF-β1-induced HK-2 epithelial-mesenchymal transition[J]. Zhongguo Zhong Yao Za Zhi, 2010, 35(1) : 89-93. Chinese with abstract in English.
  
3. Liu Q, Zhou YL, Pei Q, Yang L, Liu SK. Protection of Liuwei Dihuang pill on diabetic nephropathy in rats[J]. Hunan Zhong Yi Yao Da Xue Xue Bao, 2007, 27(6) : 40-43. Chinese with abstract in English.
4. Iwama H, Amagaya S, Ogihara Y. Effect of Shaosaikoto, a Japanese and Chinese traditional herbal medicinal mixture, on the mitogenic activity of lipopolysaccharide: a new pharmacological testing method[J]. J Ethnopharmacol, 1987, 21(1) : 45-53.
    
5. Wang WJ, Li DJ, Li J, Zhou WJ. An in vitro study on neuroprotective effects of serum containing Gengnianchun decoction and its main monomers against amyloid beta protein induced cellular toxicity[J] J Chin Integr Med, 2010, 8(1) : 67-73. Chinese with abstract in English.
  
6. Li J, Li B, Zhou WJ, Zhao FG, Li DJ, Wang WJ. Medicated rat serum containing Gengnianchun decoction reduces apoptosis of pheochromocytoma cells insulted by amyloid beta protein[J] J Chin Integr Med, 2010, 8(5) : 472-479. Chinese with abstract in English.
  
7. Okada H, Danoff TM, Kalluri R, Neilson EG. Early role of Fsp1 in epithelial-mesenchymal transformation[J]. Am J Physiol, 1997, 273(4) : F563-F574.
  
8. Bttinger EP, Bitzer M. TGF-beta1 signaling in renal disease[J]. J Am Soc Nephrol, 2002, 13(10) : 2600-2610.
    
9. Liu Y. Hepatocyte growth factor in kidney fibrosis: therapeutic potential and mechanisms of action[J]. Am J Physiol Renal Physiol, 2004, 287(1) : F7-F16.
    
10. Yang J, Dai C, Liu Y. A novel mechanism by which hepatocyte growth factor blocks tubular epithelial to mesenchymal transition[J]. J Am Soc Nephrol, 2005, 16(1) : 68-78.
    
11. Wang HJ, Jin YH, Li PY, Li LH, Bian BL. Comparison of 3 active constitutents of fresh, raw, and prepared rehmannias roots[J]. Zhongguo Zhong Yao Za Zhi, 2008, 33(15) : 1923-1925. Chinese.
  
  
Reader's comments
1. christian louboutin evening christian louboutin shoes http://christianlouboutincanadaoutlet.blogspot.com/ (2016-1-28 0:00:00)
2. ??????????????????????????????????????? (2016-3-27 0:00:00)

Send a comment

 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号