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Original Experimental Research
Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 2
Effects of the mixture of Swertia pseudochinensis Hara and Silybum marianum Gaertn extracts on CCl4-induced liver injury in rats with non-alcoholic fatty liver disease
1. Zhi-min Mao (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
2. Hai-yan Song (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
3. Li-li Yang (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
4. Tao Liu (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
5. Dong-fei Li (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
6. Pei-yong Zheng (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
7. Ping Liu (E-Institute of Shanghai Municipal Education Commission, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
8. Guang Ji (Institute of Digestive Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China E-mail: jiliver@vip.sina.com)
OBJECTIVE: To study the mechanism of liver injury induced by carbon tetrachloride (CCl4) in rats with non-alcoholic fatty liver disease (NAFLD), and the therapeutic effects of the extract mixture of Dangyao (Swertia pseudochinensis Hara) and Shuifeiji (Silybum marianum Gaertn) on NAFLD rats with liver injury.
METHODS: Male Wistar rats were randomized into normal control group, CCl4 group, high-fat diet group, high-fat diet plus CCl4 injection group (model group), diammonium glycyrrhizinate group and extract mixture group. Except the normal control and CCl4 groups, rats were fed with high-fat diet (88% normal chow, 10% lard and 2% cholesterol) to induce NAFLD. Diammonium glycyrrhizinate and extracts were given by gavage. After eight weeks, a nonlethal dose of CCl4 was injected intraperitoneally to all rats except the normal and high-fat diet groups. And 48 h later, all rats were sacrificed, and serum and liver tissues were collected for further study. Paraffin-processed liver tissue was stained with hematoxylin-eosin (HE) to observe the pathological changes. Serum alamine aminotrasferas (ALT) and aspartate aminotransferase (AST) were measured. The levels of triacylglycerol (TAG), malondialdehyde (MDA) and glutathione (GSH) in liver tissues were also examined. Expression of uncoupling protein 2 (UCP2) was determined by reverse transcription-polymerase chain reaction and Western blotting.
RESULTS: Liver sections stained with HE showed that the histopathological changes in the normal control group and the CCl4 group were mild; massive hepatosteatosis diffusing in lobules was shown in the high-fat diet groups; steatosis, hepatocellular ballooning degeneration and inflammatory infiltration were severe around the central vein in sections of the model group. Compared with the model group, hepatosteatosis and ballooning were significantly attenuated in the treatment groups. Levels of serum ALT and AST, contents of TAG and MDA and the UCP2 expression in liver tissues of the model group increased obviously, while the level of liver GSH decreased. Compared with rats in the model group, the above biomarkers in the treatment groups were improved significantly.
CONCLUSION: The mixture of Dangyao and Shuifeiji extracts can decrease the susceptibility and degree of liver injury induced by hepatotoxin in rats with NAFLD. Regulation of the balance of pro- and anti-oxidative stress factors is involved in the mechanism.

Received September 1, 2011; accepted October 26, 2011; published online February 15, 2012.
Full-text LinkOut at PubMed. Journal title in PubMed: Zhong Xi Yi Jie He Xue Bao.

基金项目:上海市教育委员会重点学科建设项目(No. J50305); 上海市科学技术委员会自然基金资助项目(No. 11ZR1436900); 上海市教育委员会预算内科研项目(No. 2010JW35); 上海高校创新团队建设项目(第一期)
Correspondence: Guang Ji, MD, Professor; Tel: 021-64286261; E-mail: jiliver@vip.sina.com

Full text of this article is in Chinese

  
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