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Original Experimental Research
Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 3
Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice
1. Shan Gao (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
2. Xing-hui Wang (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
3. Ling-ling Huang (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
4. Ting-ting Yu (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
5. Su-ming Du (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
6. Yan-wei Guo (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
7. Yuan Jia (Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China )
8. Jian Wang (Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China E-mail: wangjian6301@163.com)
OBJECTIVE: To investigate the effects of Xinji’erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
METHODS: Isoproterenol was given subcutaneously (1 mg/kg, twice per day for 7 d) to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, XJEK low-, medium- and high-dose groups, XJEK water layer group, XJEK n-butanol layer group and metoprolol group. All drugs were given by intragastric administration. At the end of the 7th day, the hearts of the rats were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight (HW/BW). The histological changes were observed by hemotoxylin-eosin and Van Gieson staining. Colorimetric method was used to determine the content of hydroxyproline in heart, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum.
RESULTS: Compared with the isoproterenol injection only, XJEK potently inhibited cardiomyocyte hypertrophy and the increase of hydroxyproline content in heart (P<0.01), improved cardiac pathology change, inhibited the decrease of SOD activity and the increase of MDA content in serum (P<0.01). XJEK water layer also inhibited the increase of cardiomyocyte hypertrophy (P<0.01) while XJEK n-butanol layer inhibited cardiomyocyte hypertrophy and fibrosis (P<0.01).
CONCLUSION: XJEK possesses protective effects against isoproterenol-induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body. XJEK water layer and XJEK n-butanol layer attenuated ventricular remodeling without significant oxidative stress state changing, which indicates that a non-antioxidative stress mechanism may exist.

Received September 21, 2011; accepted November 9, 2011; published online March 15, 2012.
Full-text LinkOut at PubMed. Journal title in PubMed: Zhong Xi Yi Jie He Xue Bao.

基金项目:国家自然科学基金资助项目(No. 81073088);教育部高等学校博士学科点专项科研基金资助项目(No. 20103420120002);安徽医科大学博士启动基金资助项目(No. XJ200709)
Correspondence: Jian Wang, MD, Professor; E-mail: wangjian6301@163.com

Full text of this article is in Chinese

  
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