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Original Experimental Research
Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 4
Salvianolic acid B inhibits ERK signal transduction pathway activated by transforming growth factor-β1 in rat hepatic stellate cells
1. Ya-fang Song (Department of Liver Cirrhosis, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
2. Zhi-gang Lü (Department of Liver Cirrhosis, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
3. Lie-ming Xu (Department of Liver Cirrhosis, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China E-mail: E-mail: xulieming@shutcm.edu.cn)
OBJECTIVE: To investigate the effects of salvianolic acid B (SA-B) on extracellular signal-regulated kinase (ERK) signal transduction pathway activated by transforming growth factor-β1 (TGF-β1) in rat hepatic stellate cells (HSCs).
METHODS: HSCs were isolated from male Sprague-Dawley rats by in situ perfusion and Nycodenz density-gradient centrifugation method. TGF-β1 and SA-B were directly added to the serum-free medium of HSCs. Total and phosphorylated ERK, MEK, Raf and α-smooth muscle actin (α-SMA) and typeⅠcollagen were assayed by Western blotting.
RESULTS: Phosphorylation of MEK in HSCs with or without TGF-β1 was inhibited by SA-B; however, phosphorylation of Raf in HSCs with or without TGF-β1 was not inhibited by SA-B. Expression of α-SMA in HSCs with TGF-β1 was inhibited by SA-B. Combination of SA-B and the inhibitors of ERK (PD98059) can effectively inhibit the expression of α-SMA. SA-B also inhibited synthesization of typeⅠcollagen in HSCs with or without TGF-β1.
CONCLUSION: The action point of SA-B inhibiting ERK signaling induced by TGF-β1 in HSCs is the inhibition of the phosphorylation of MEK. SA-B reduces the increase of expression of α-SMA and protein synthesization of type Ⅰ collagen induced by TGF-β1 by means of inhibiting ERK signaling in activated HSCs of rats.

Received August 18, 2011; accepted September 15, 2011; published online April 15, 2012.
Full-text LinkOut at PubMed. Journal title in PubMed: Zhong Xi Yi Jie He Xue Bao.

基金项目:国家自然科学基金资助项目(No. 30271657); 上海市教育委员会重点学科建设项目(No. 04CA02); 上海市重点学科建设项目(No. Y0302); 上海市教育委员会重点学科(第五期)建设项目(No. J50307); 上海市高校E-研究院资助项目(No. E03008); 上海市高校创新团队建设资助项目(第一期)
Correspondence: Lie-ming Xu, MD, Professor; Tel: 021-20256520; E-mail: xulieming@shutcm.edu.cn

Full text of this article is in Chinese

  
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