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Journal of Integrative Medicine: Volume 15, 2017   Issue 5,  Pages: 398–406

DOI: 10.1016/S2095-4964(17)60359-5
Research Article
Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents
1. Javed Yakoob (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
2. Zaigham Abbas (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
3. Malik Hassan Mehmood (Department of Biological Biomedical Sciences, Aga Khan University, Karachi 74800, Pakistan )
4. Kanwal Tariq (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
5. Saima Azhar Saleem (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
6. Safia Awan (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
7. Abdul Malik (Department of Biological Biomedical Sciences, Aga Khan University, Karachi 74800, Pakistan )
8. Saeed Hamid (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
9. Rustam Khan (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
10. Wasim Jafri (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
ABSTRACT OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (HopQ) mediates host-pathogen interactions; HopQ genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against HopQ genotypes and H. pylori cytotoxin-associated gene A (CagA). METHODS: DNA was extracted by polymerase chain reaction of the HopQ genotypes (i.e., type 1, type 2 and CagA) from 115 H. pylori strains. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis. RESULTS: C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% ± 3.7%, 13.8% ± 3.6%, respectively, against HopQ type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against HopQ type 2. C. longa (93%), C. annum (89%) and A. esculentus (75%) had better anti-adhesive activity against H. pylori with HopQ type 1 compared to HopQ type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum (14.7% ± 4.1%), A. esculentus (12.3% ± 4.1%) and Z. officinale (8.4% ± 2.8%) had an anti-adhesion effect against CagA-positive H. pylori strains compared to CagA-negative strains. CONCLUSION: The anti-adhesion properties of the tested phytotherapeutic dietary ingredients were varied with HopQ genotypes. HopQ type 1 was found to be more sensitive to extracts of C. annum, C. longa and A. esculentus compared to the HopQ type 2 genotype. Keywords: outer membrane protein Q; Helicobacter pylori; anti-adhesion; Abelmoschus esculentus; Capsicum annum; Curcuma longa
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Please cite this article as:
Yakoob J, Abbas Z, Mehmood MH, Tariq K, Saleem SA, Awan S, Malik A, Hamid S, Khan R, Jafri W. Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents. J Integr Med. 2017; 15(5): 398–406.
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