Updated Tuesday, October 24, 2017
 Journal Tools
RSS Feed
Sample Copy
Submit a Manuscript
Contact Us
Subscription
Advertising
Thanking Peer Reviewers
 Language Polishing
Journal of Integrative Medicine: Volume 15, 2017   Issue 5,  Pages: 398–406

DOI: 10.1016/S2095-4964(17)60359-5
Research Article
Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents
1. Javed Yakoob (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
2. Zaigham Abbas (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
3. Malik Hassan Mehmood (Department of Biological Biomedical Sciences, Aga Khan University, Karachi 74800, Pakistan )
4. Kanwal Tariq (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
5. Saima Azhar Saleem (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
6. Safia Awan (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
7. Abdul Malik (Department of Biological Biomedical Sciences, Aga Khan University, Karachi 74800, Pakistan )
8. Saeed Hamid (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
9. Rustam Khan (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
10. Wasim Jafri (Department of Medicine, Aga Khan University, Karachi 74800, Pakistan )
ABSTRACT OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (HopQ) mediates host-pathogen interactions; HopQ genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against HopQ genotypes and H. pylori cytotoxin-associated gene A (CagA). METHODS: DNA was extracted by polymerase chain reaction of the HopQ genotypes (i.e., type 1, type 2 and CagA) from 115 H. pylori strains. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis. RESULTS: C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% ± 3.7%, 13.8% ± 3.6%, respectively, against HopQ type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against HopQ type 2. C. longa (93%), C. annum (89%) and A. esculentus (75%) had better anti-adhesive activity against H. pylori with HopQ type 1 compared to HopQ type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum (14.7% ± 4.1%), A. esculentus (12.3% ± 4.1%) and Z. officinale (8.4% ± 2.8%) had an anti-adhesion effect against CagA-positive H. pylori strains compared to CagA-negative strains. CONCLUSION: The anti-adhesion properties of the tested phytotherapeutic dietary ingredients were varied with HopQ genotypes. HopQ type 1 was found to be more sensitive to extracts of C. annum, C. longa and A. esculentus compared to the HopQ type 2 genotype. Keywords: outer membrane protein Q; Helicobacter pylori; anti-adhesion; Abelmoschus esculentus; Capsicum annum; Curcuma longa
Welcome to JIM! You are the number 199 reader of this article!
Download Article:
[Full Text]      [PDF]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Yakoob J, Abbas Z, Mehmood MH, Tariq K, Saleem SA, Awan S, Malik A, Hamid S, Khan R, Jafri W. Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents. J Integr Med. 2017; 15(5): 398–406.
References:
1Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J].Int J Cancer, 2010, 127(12): 2893–2917.  
2Shiotani A, Lu H, Dore MP, Graham DY. Treating Helicobacter pylori effectively while minimizing misuse of antibiotics[J].Cleve Clin J Med, 2017, 84(4): 310–318.  
3Tegtmeyer N, Wessler S, Backert S. Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis[J].FEBS J, 2011, 278(8): 1190–1202.  
4Palframan SL, Kwok T, Gabriel K. Vacuolating cytotoxin A (VacA), a key toxin for Helicobacter pylori pathogenesis[J].Front Cell Infect Microbiol, 2012, 2: 92.  
5Skoog EC, Padra M, ?berg A, Gideonsson P, Obi I, Quintana-Hayashi MP, Arnqvist A, Lindén SK. BabA dependent binding of Helicobacter pylori to human gastric mucins causes aggregation that inhibits proliferation and is regulated via ArsS[J].Sci Rep, 2017, 7: 40656.  
6Harvey VC, Acio CR, Bredehoft AK, Zhu L, Hallinger DR, Quinlivan-Repasi V, Harvey SE, Forsyth MH. Repetitive sequence variations in the promoter region of the adhesin-encoding gene sabA of Helicobacter pylori affect transcription[J].J Bacteriol, 2014, 196(19): 3421–3429.  
7Zhang J, Qian J, Zhang X, Zou Q. Outer membrane inflammatory protein A, a new virulence factor involved in the pathogenesis of Helicobacter pylori[J].Mol Biol Rep, 2014, 41(12): 7807–7814.  
8Lu H, Wu JY, Beswick EJ, Ohno T, Odenbreit S, Haas R, Reyes VE, Kita M, Graham DY, Yamaoka Y. Functional and intracellular signaling differences associated with the Helicobacter pylori AlpAB adhesin from Western and East Asian strains[J].J Biol Chem, 2007, 282(9): 6242–6254.  
9Cao P, Cover TL. Two different families of hopQ alleles in Helicobacter pylori[J].J Clin Microbiol, 2002, 40(12): 4504–4511.  
10Oleastro M, Ménard A. The role of Helicobacter pylori outer membrane proteins in adherence and pathogenesis[J].Biology (Basel), 2013, 2(3): 1110–1134.  
11Senkovich OA, Yin J, Ekshyyan V, Conant C, Traylor J, Adegboyega P, McGee DJ, Rhoads RE, Slepenkov S, Testerman TL. Helicobacter pylori AlpA and AlpB bind host laminin and influence gastric inflammation in Gerbils[J].Infect Immun, 2011, 79(8): 3106–3116.  
12Loh JT, Torres VJ, Algood HM, McClain MS, Cover TL. Helicobacter pylori HopQ outer membrane protein attenuates bacterial adherence to gastric epithelial cells[J].FEMS Microbiol Lett, 2008, 289(1): 53–58.  
13Belogolova E, Bauer B, Pompaiah M, Asakura H, Brinkman V, Ertl C, Bartfeld S, Nechitaylo TY, Haas R, Machuy N, Salama N, Churin Y, Meyer TF. Helicobacter pylori outer membrane protein HopQ identified as a novel T4SS-associated virulence factor[J].Cell Microbiol, 2013, 15(11): 1896–1912.  
14Oleastro M, Cordeiro R, Ferrand J, Nunes B, Lehours P, Carvalho-Oliveira I, Mendes AI, Penque D, Monteiro L, Mégraud F, Ménard A. Evaluation of the clinical significance of homB, a novel candidate marker of Helicobacter pylori strains associated with peptic ulcer disease[J].J Infect Dis, 2008, 198(9): 1379–1387.  
15Cao P, Lee KJ, Blaser MJ, Cover TL. Analysis of hopQ alleles in East Asian and Western strains of Helicobacter pylori[J].FEMS Microbiol Lett, 2005, 251(1): 37–43.  
16Yakoob J, Abbas Z, Khan R, Salim SA, Awan S, Abrar A, Jafri W. Helicobacter pylori outer membrane protein Q allele distribution is associated with distinct pathologies in Pakistan[J].Infect Genet Evol, 2016, 37: 57–62.  
17Burger O, Ofek I, Tabak M, Weiss EI, Sharon N, Neeman I. A high molecular mass constituent of cranberry juice inhibits Helicobacter pylori adhesion to human gastric mucus[J].FEMS Immunol Med Microbiol, 2000, 29(4): 295–301.  
18Yakoob J, Hu GL, Fan XG, Yang HX, Liu SH, Tan DM, Li TG, Zhang Z. Diversity of Helicobacter pylori among Chinese persons with H. pylori infection[J].APMIS, 2000, 108(7–8): 482–486.  
19Lee A, Megraud F. Helicobacter pylori: techniques for clinical diagnosis and basic research[M]. Philadelphia: Saunders (W.B.) Co Ltd, 1995. 94–109.  
20Williamson EM, Okpako DT, Evans FJ. Pharmacological methods in phytotherapy research[M]. Chichester: John Wiley & Sons Ltd, 1996. 15–23.  
21Sellins KS, Cohen JJ. Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes[J].J Immunol, 1987, 139(10): 3199–3206.  
22Zaidi SF, Muhammad JS, Shahryar S, Usmanghani K, Gilani AH, Jafri W, Sugiyama T. Anti-inflammatory and cytoprotective effects of selected Pakistani medicinal plants in Helicobacter pylori-infected gastric epithelial cells[J].J Ethnopharmacol, 2012, 141(1): 403–410.  
23Zaidi SF, Yamamoto T, Refaat A, Ahmed K, Sakurai H, Saiki I, Kondo T, Usmanghani K, Kadowaki M, Sugiyama T. Modulation of activation-induced cytidine deaminase by curcumin in Helicobacter pylori-infected gastric epithelial cells[J].Helicobacter, 2009, 141(1): 588–595.  
24Meyer F, Wilson KT, James SP. Modulation of innate cytokine responses by products of Helicobacter pylori[J].Infect Immun, 2000, 68(11): 6265–6272.  
25Cwikla C, Schmidt K, Matthias A, Bone KM, Lehmann R, Tiralongo E. Investigations into the antibacterial activities of phytotherapeutics against Helicobacter pylori and Campylobacter jejuni[J].Phytother Res, 2010, 24(5): 649–656.  
26Gaus K, Huang Y, Israel DA, Pendland SL, Adeniyi BA, Mahady GB. Standardized ginger (Zingiber officinale) extract reduces bacterial load and suppresses acute and chronic inflammation in Mongolian gerbils infected with CagA Helicobacter pylori[J].Pharm Biol, 2009, 47(1): 92–98.  
27Mahady GB, Pendland SL, Yun GS, Lu ZZ, Stoia A. Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of CagA+ strains of Helicobacter pylori[J].Anticancer Res, 2003, 23(5A): 3699–3702.  
 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号