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Journal of Integrative Medicine: Volume 16, 2018   Issue 1,  Pages: 51-56

DOI: 10.1016/j.joim.2017.11.001
Original Experimental Research
Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester-induced hypertensive male rats
1. Shakiru Ademola Salami (Department of Physiology, Lagos State University College of Medicine, Lagos State, Ikeja 23401, Nigeria )
2. Hussein Mofomosara Salahdeen (Department of Physiology, Lagos State University College of Medicine, Lagos State, Ikeja 23401, Nigeria )
3. Evangelshane Chukwudubem Ugbebor (Department of Physiology, Lagos State University College of Medicine, Lagos State, Ikeja 23401, Nigeria )
4. Babatunde Adekunle Murtala (Department of Physiology, Lagos State University College of Medicine, Lagos State, Ikeja 23401, Nigeria )
5. Yinusa Raji (Laboratory for Reproductive Physiology and Developmental Programming, Department of Physiology, University of Ibadan, Ibadan 23402, Nigeria )

 Abstract

Objective
This study investigated the effects of aqueous leaf extract of Tridax procumbens (ALETP) on contractile activity of corpus cavernosum in N-nitro-l-arginine methyl ester (l-NAME)-induced hypertensive male rats.
Methods
Twenty normal, adult male rats (130–150 g) were divided into four groups of five rats each. Group I (control) was given normal saline (0.6 mL/kg) and group II was given l-NAME (40 mg/kg) for 6 weeks. Groups III and IV also received l-NAME (40 mg/kg) for 6?weeks but were further co-treated with 100 and 200?mg/kg of ALETP, respectively, from week 4 to week 6. All treatments were given orally. Strips of corpus cavernosum from each of the four groups were exposed to increasing concentrations of acetylcholine (ACh) and sodium nitroprusside (SNP) (10−9–10−5 mol/L) after contraction with phenylephrine (10−7 mol/L) to test for a dose–response effect. Response to potassium and calcium was also measured after cumulatively adding potassium and calcium (10–50 mmol/L) to potassium- and calcium-free organ chamber. Isometric contractions were recorded through an Ugo Basile data capsule acquisition system.
Results
Mean arterial blood pressure was significantly reduced in the ALETP co-treated group compared to the control and
l-NAME-only groups (P < 0.05). Cavernosa strips from ALETP co-treated rats exhibited significant inhibition of contraction in response to phenylephrine, potassium chloride, and calcium chloride (P < 0.05). Relaxation in response to Ach and SNP was also significantly impaired in cavernosa strips from the l-NAME-only treated group (P < 0.05), while ALETP co-treated groups showed enhanced percentage relaxation.
Conclusion
ALETP treatment of l-NAME-induced hypertensive rats promotes a relaxant effect on isolated cavernosa strips. ALETP shows potential in correcting erectile dysfunction in hypertension.

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Please cite this article as:
Salami SA, Salahdeen HM, Ugbebor EC, Murtala BA, Raji Y. Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester-induced hypertensive male rats. J Integr Med. 2017 December; Epub ahead of print.
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