Updated Thursday, April 25, 2019
 Journal Tools
RSS Feed
Sample Copy
Submit a Manuscript
Contact Us
Subscription
Advertising
Thanking Peer Reviewers
 Language Polishing
Journal of Integrative Medicine: Volume 17, 2019   Issue 2,  Pages: 132-140

DOI: 10.1016/j.joim.2019.02.001
Original Experimental Research
Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development
1. Carlos R.Oliveira (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
2. Daniel G.Spindola (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
3. Daniel M.Garcia (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
4. Adolfo Erustes (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
5. Alexandre Bechara (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
6. Caroline Palmeira-dos-Santos (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
7. Soraya S.Smaili (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
8. Gustavo J.S. Pereira (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )
9. André Hinsberger (Grupo de Fitocomplexos e Sinaliza??o Celular, Escola de Ciências da Saúde, Universidade Anhembi Morumbi, S?o Paulo, SP 03164-000, Brazil )
10. Ezequiel P. Viriato (Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz, S?o Paulo, SP 01151-000, Brazil )
11. Maria Cristina Marcucci (Laboratório de Produtos Naturais e Quimiometria, Programa de pós-gradua??o em Farmácia e Biotecnologia, Universidade Anhanguera de S?o Paulo, S?o Paulo, SP 05145-200, Brazil )
12. Alexandra C.H.F. Sawaya (Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP 13083-871, Brazil )
13. Samantha L. Tomaz (Unidade de Oncologia Experimental, EPM, Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04023-901, Brazil )
14. Elaine G. Rodrigues (Unidade de Oncologia Experimental, EPM, Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04023-901, Brazil )
15. Claudia Bincoletto (Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de S?o Paulo (UNIFESP), S?o Paulo, SP 04044-020, Brazil )

ABSTRACT
Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its root. This study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root (CEAA) on breast cancer.
Methods: The cytotoxicity of CEAA against breast adenocarcinoma cells (4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide (PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4NW. Immunoblotting was used to determine the effect of CEAA on anti- and pro-apoptotic proteins (Bcl-2 and Bax, respectively). The 4T1 cell-challenged mice were used for in vivo assay.
Results: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study (73 µg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA (500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated animals.
Conclusion: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy.

Welcome to JIM! You are the number 85 reader of this article!
Download Article:
[Full Text]      [PDF]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Carlos R.Oliveira, Daniel G.Spindola, Daniel M.Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S.Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. J Integr Med. 2019; 17(2): 132-140.
 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号