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Journal of Integrative Medicine: Volume 17, 2019   Issue 3,  Pages: 213-220

DOI: 10.1016/j.joim.2019.02.004
Research Article
Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements
1. Stefania Lamponi (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy E-mail: stefania.lamponi@unisi.it)
2. Anna Maria Aloisi (Union B.I.O. S.r.l., 52100 Arezzo, Italy )
3. Claudia Bonechi (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
4. Marco Consumi (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
5. Alessandro Donati (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
6. Gemma Leone (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
7. Claudio Rossi (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
8. Gabriella Tamasi (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )
9. Luana Ghiandai (Union B.I.O. S.r.l., 52100 Arezzo, Italy )
10. Ersilia Ferrini (Union B.I.O. S.r.l., 52100 Arezzo, Italy )
11. Paolo Fiorenzani (Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy )
12. Ilaria Ceccarelli (Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy )
13. Agnese Magnani (1.Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy )

ABSTRACT

Objective: In vitro cell and blood compatibility of three dietary supplements, comprised of multiple plant extracts, Pneumo Go (PG), Green active (GA) and Equistasi (Eq), and their main component, the phytocomplex Matrix Union Bio S.r.l.® (M), were evaluated. Moreover, preliminary in vivo tests were performed on GA in order to assess its ability to reduce pain in an animal model.

Methods: Cell compatibility was determined using fibroblasts (NIH3T3) and primary adult human microvascular endothelial cells (HMVECad) and the neutral red uptake test. Blood compatibility was evaluated by analysing blood parameters after incubation of the products with sodium citrate anticoagulated whole blood. Thrombin time was determined by adding thrombin to aliquots of human plasma containing the samples. Clotting time was revealed by an automatic coagulometer. The in vivo analgesic effect of GA was evaluated in Wistar rats using the formalin test.

Results: M and PG reduced the percentage of viable NIH3T3 cells, indicating their interference in the cell cycle. GA and Eq stimulated fibroblast proliferation and neutralized the toxic effect of M. M and PG reduced HMVECad cell viability. GA and Eq did not affect cell viability as well as negative control. The hemocompatibility tests indicated that all the samples did not interfere with fibrinogen. The in vivo test carried out in male rats showed a significant analgesic effect of GA in all formalin-induced pain behaviors.

Conclusion: No hemotoxicity and good cell compatibility were found for all the tested samples. GA and Eq were the best candidates for further biocompatibility testing. Moreover, GA reduced pain in the animal model.

 

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Please cite this article as:
Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. J Integr Med. 2019; 17(3): 213-220.
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