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Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 2,  Pages: 210-227

DOI: 10.3736/jcim20120212
Original Experimental Research
Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenite-exposed bacteria Escherichia coli
1. Arnab De (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
2. Durba Das (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
3. Suman Dutta (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
4. Debrup Chakraborty (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
5. Naoual Boujedaini (Boiron Laboratory, Lyon, France )
6. Anisur Rahman Khuda-Bukhsh (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India E-mail: khudabukhsh_48@rediffmail.com)
OBJECTIVE: To examine if potentized homeopathic drug Arsenicum Album 30C (Ars Alb 30C) can reduce sodium arsenite-induced toxicity in Escherichia coli.
METHODS: E. coli were exposed to low arsenite insult after they grew up to log phase in standard Luria-Bertani medium. E. coli were treated with 1 or 2 mmol/L sodium arsenite alone (control), or Ars Alb 30C was added to the medium of a subset of sodium arsenite-treated bacteria (drug-treated), or homeopathically agitated alcohol was added to the medium containing a subset of sodium arsenite-treated bacteria (placebo-treated). A sub-set of untreated E. coli served as the negative control. Glucose uptake, specific activities of hexokinase, lipid peroxidase (LPO), superoxide dismutase (SOD) and catalase, intra- and extra-cellular sodium arsenite content, cell growth, cell membrane potential, DNA damage, intracellular reactive oxygen species (ROS), adenosine triphosphate (ATP) and free glutathione content and expressions of arsB and ptsG gene in normal control, sodium arsenite-treated, drug-treated and placebo-treated E. coli were analyzed. Treatments were blinded and randomized.
RESULTS: In sodium arsenite-treated E. coli, glucose uptake, intracellular ROS, LPO and DNA damage increased along with decrease in the specific activities of hexokinase, SOD and catalase, intracellular ATP and free glutathione contents and cell membrane potential and growth, and there were increases in expression levels of arsB gene and ptsG gene. Ars Alb 30C administration reduced arsenic toxicity in E. coli by inhibiting generation of ROS and increasing tolerance to arsenite toxicity and cell growth.
CONCLUSION: Ars Alb 30C ameliorated arsenic toxicity and DNA damage, validating efficacy of ultra-highly diluted remedies used in homeopathy.
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De A, Das D, Dutta S, Chakraborty D, Boujedaini N, Khuda-Bukhsh AR. Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenine-exposed bacteria Escherichia coli. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(2): 210-227.
1Chikramane PS, Suresh AK, Bellare JR, Govind S. Extreme homeopathic dilutions remain starting materials: a nanoparticulate perspective[J].Homeopathy, 2010, 99(4): 231-242.  
2Vickers AJ, Fisher P, Smith C, Wyllie SE, Rees R. Homeopathic Arnica 30× is ineffective for muscle soreness after long-distance running: a randomized, double-blind, placebo-controlled trial[J].Clin J Pain, 1998, 14(3): 227-231.  
3Lewith GT, Watkins AD, Hyland ME, Shaw S, Broomfield JA, Dolan G, Holgate ST. Use of ultramolecular potencies of allergen to treat asthmatic people allergic to house dust mite: double blind randomised controlled clinical trial[J].BMJ, 2002, 324(7336): 520.  
4Ernst E. A systematic review of systematic reviews of homeopathy[J].Br J Clin Pharmacol, 2002, 54(6): 577-582.  
5Shang A, Huwiler-Müntener K, Nartey L, Jüni P, Drig S, Sterne JA, Pewsner D, Egger M. Are the clinical effects of homoeopathy placebo effects? Comparative study of placebo-controlled trials of homoeopathy and allopathy[J].Lancet, 2005, 366(9487): 726-732.  
6House of Commons Science and Technology Committee. Evidence check 2: homeopathy. Fourth report of session 2009-10: Report, together with formal minutes, oral and written evidence. London: The Stationery Office Limited. 2010.
7Khuda-Bukhsh AR, De A, Das D, Dutta S, Boujedaini N. Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia coli. J Chin Integr Med. 2011; 9(8): 901-912. English with abstract in Chinese.
8Lewin B. Genes Ⅷ. Upper Saddle River: Pearson Prentice Hall Pearson Education, Inc. 2004.
9Silver S, Budd K, Leahy KM, Shaw WV, Hammond D, Novick RP, Willsky GR, Malamy MH, Rosenberg H. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (Ⅲ) in[J].J Bacteriol, 1981, 146(3): 983-996.  
10Mokrasch LC. Analysis of hexose phosphates and sugar mixtures with the anthrone reagent[J].J Biol Chem, 1954, 208(1): 55-59.  
11Racker E. Spectrophotometric measurement of hexokinase and phosphohexokinase activity[J].J Biol Chem, 1947, 167(3): 843-854.  
12Kangas L, Grnroos M, Nieminen AL. Bioluminescence of cellular ATP: a new method for evaluating cytotoxic agents in vitro[J].Med Biol, 1984, 62(6): 338-343.  
13O’Keefe D, Collier RJ. Cloned diphtheria toxin within the periplasm of Escherichia coli causes lethal membrane damage at low pH[J].Proc Natl Acad Sci U S A, 1989, 86(1): 343-346.  
14Amor KB, Breeuwer P, Verbaarschot P, Rombouts FM, Akkermans AD, De Vos WM, Abee T. Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress[J].Appl Environ Microbiol, 2002, 68(11): 5209-5216.  
15Behari JR, Prakash R. Determination of total arsenic content in water by atomic absorption spectroscopy (AAS) using vapour generation assembly (VGA)[J].Chemosphere, 2006, 63(1): 17-21.  
16Chowdhury R, Dutta A, Chaudhuri SR, Sharma N, Giri AK, Chaudhuri K. In vitro and in vivo reduction of sodium arsenite induced toxicity by aqueous garlic extract[J].Food Chem Toxicol, 2008, 46(2): 740-751.  
17Bagchi P, Dutta D, Chattopadhyay S, Mukherjee A, Halder UC, Sarkar S, Kobayashi N, Komoto S, Taniguchi K, Chawla-Sarkar M. Rotavirus nonstructural protein 1 suppresses virus-induced cellular apoptosis to facilitate viral growth by activating the cell survival pathways during early stages of infection[J].J Virol, 2010, 84(13): 6834-6845.  
18Buege JA, Aust SD. Microsomal lipid peroxidation[J].Methods Enzymol, 1978, 52: 302-310.  
19Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase[J].Indian J Biochem Biophys, 1984, 21(2): 130-132.  
20Chance B, Maehly AC. Assay of catalases and peroxidases[J].Methods Enzymol, 1955, 2: 764-775.  
21Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent[J].Anal Biochem, 1968, 25(1): 192-205.  
22Dhiman R, Kathania M, Raje M, Majumdar S. Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification[J].Biochim Biophys Acta, 2008, 1780(4): 733-742.  
23Dhawan A, Mathur N, Seth PK. The effect of smoking and eating habits on DNA damage in Indian population as measured in the comet assay[J].Mutat Res, 2001, 474(1-2): 121-128.  
24Stock-Schrer B, Albrecht H, Betti L, Dobos G, Endler C, Linde K, Lüdtke R, Musial F, van Wijk R, Witt C, Baumgartner S. Reporting experiments in homeopathic basic research — description of the checklist development. Evid Based Complement Alternat Med. 2009 Nov 1. [Epub ahead of print]
25Suntres ZE. Role of antioxidants in paraquat toxicity[J].Toxicology, 2002, 180(1): 65-77.  
26Lü Z, Min H, Xia Y. The response of Escherichia coli, Bacillus subtilis, and Burkholderia cepacia WZ1 to oxidative stress of exposure to quinclorac[J].J Environ Sci Health B, 2004, 39(3): 431-441.  
27Sies H, Menck CF. Singlet oxygen induced DNA damage[J].Mutat Res, 1992, 275(3-6): 367-375.  
28Sies H. Damage to plasmid DNA by singlet oxygen and its protection[J].Mutat Res, 1993, 299(3-4): 183-191.  
29Yao XH, Min H, Lv ZM. Response of superoxide dismutase, catalase, and ATPase activity in bacteria exposed to acetamiprid[J].Biomed Environ Sci, 2006, 19(4): 309-314.  
30Imlay JA, Chin SM, Linn S. Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro[J].Science, 1988, 240(4852): 640-642.  
31Imlay JA, Linn S. DNA damage and oxygen radical toxicity[J].Science, 1988, 240(4857): 1302-1309.  
32Chen JR, Weng CN, Ho TY, Cheng IC, Lai SS. Identification of the copper-zinc superoxide dismutase activity in Mycoplasma hyopneumoniae[J].Vet Microbiol, 2000, 73(4): 301-310.  
33Lehmann Y, Meile L, Teuber M. Rubrerythrin from Clostridium perfringens: cloning of the gene, purification of the protein, and characterization of its superoxide dismutase function[J].J Bacteriol, 1996, 178(24): 7152-7158.  
34Gerlach D, Reichardt W, Vettermann S. Extracellular superoxide dismutase from Streptococcus pyogenes type 12 strain is manganese-dependent[J].FEMS Microbiol Lett, 1998, 160(2): 217-224.  
35Jung S. Expression level of specific isozymes of maize catalase mutants influences other antioxidants on norflurazon-induced oxidative stress[J].Pestic Biochem Physiol, 2003, 75(1-2): 9-17.  
36Cabiscol E, Tamarit J, Ros J. Oxidative stress in bacteria and protein damage by reactive oxygen species[J].Int Microbiol, 2000, 3(1): 3-8.  
37Rey L. Thermoluminescence of ultra-high dilutions of lithium chloride and sodium chloride[J].Physica A, 2003, 323: 67-74.  
38Rey L. Can low-temperature thermoluminescence cast light on the nature of ultra-high dilutions?[J].Homeopathy, 2007, 96(3): 170-174.  
39Khuda-Bukhsh AR. Potentized homoeopathic drugs act through regulation of gene-expression: a hypothesis to explain their mechanism and pathways of action in vitro[J].Complement Ther Med, 1997, 5(1): 43-46.  
40Khuda-Bukhsh AR. Expression level of specific isozymes of maize catalase mutants influences other antioxidants on nonflurazon-induced oxydative stress[J].Mol Cell Biochem, 2003, 253(1-2): 339-345.  
41Khuda-Bukhsh AR. Laboratory research in homeopathy: pro[J].Integr Cancer Ther, 2006, 5(4): 320-332.  
42Khuda-Bukhsh AR. Mice as a model for homeopathy research[J].Homeopathy, 2009, 98(4): 267-279.  
43Khuda-Bukhsh AR, Pathak S. Homeopathic drug discovery: theory update and methodological aspect[J].Expert Opin Drug Discov, 2008, 3(8): 979-990.  
44Bhattacharyya SS, Paul S, Mandal SK, Banerjee A, Boujedaini N, Khuda-Bukhsh AR. A synthetic coumarin (4-methyl-7 hydroxy coumarin) has anti-cancer potentials against DMBA-induced skin cancer in mice[J].Eur J Pharmacol, 2009, 614(1-3): 128-136.  
45Mallick P, Mallick JC, Guha B, Khuda-Bukhsh AR. Ameliorating effect of microdoses of a potentized homeopathic drug, Arsenicum Album, on arsenic-induced toxicity in mice[J].BMC Complement Altern Med, 2003, 3: 7.  
46Banerjee A, Pathak S, Biswas SJ, Roy-Karmakar S, Boujedaini N, Belon P, Khuda-Bukhsh AR. Chelidonium majus 30C and 200C in induced hepato-toxicity in rats[J].Homeopathy, 2010, 99(3): 167-176.  
47Pathak S, Kumar Das J, Jyoti Biswas S, Khuda-Bukhsh AR. Protective potentials of a potentized homeopathic drug, Lycopodium-30, in ameliorating azo dye induced hepatocarcinogenesis in mice[J].Mol Cell Biochem, 2006, 285(1-2): 121-131.  
48Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Dutta S, Boujedaini N, Belon P. Modulation of signal proteins: a plausible mechanism to explain how a potentized drug secale cor 30C diluted beyond Avogadro’s limit combats skin papilloma in mice. Evid Based Complement Alternat Med. 2009 Jul 16. [Epub ahead of print]
49Center of Conciousness Studies, The University of Arizona. Proceedings of the eighteenth annual international, interdisciplinary conference on the fundamental question of how the brain produces conscious experience. Stockholm, Sweden, May 3-7, 2011.
50Das D, De A, Dutta S, Biswas R, Boujedaini N, Khuda-Bukhsh AR. Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate[J]. J Chin Integr Med, 2011, 9(7): 752-760. Chinese
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