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Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 2,  Pages: 228-232

DOI: 10.3736/jcim20120213
Original Experimental Research
Antihyperglycemic and antinociceptive activities of methanolic extract of Euphorbia thymifolia L. whole plants
1. Mohammed Rahmatullah (Department of Biotechnology and Genetic Engineering, Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka 1205, Bangladesh E-mail: rahamatm@hotmail.com)
2. Sumaiyah Kanij Hasan (Department of Biotechnology and Genetic Engineering, Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka 1205, Bangladesh )
3. Zulfiquar Ali (Department of Biotechnology and Genetic Engineering, Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka 1205, Bangladesh )
4. Shahnaz Rahman (Department of Biotechnology and Genetic Engineering, Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka 1205, Bangladesh )
5. Rownak Jahan (Department of Biotechnology and Genetic Engineering, Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka 1205, Bangladesh )
OBJECTIVE: To study the antihyperglycemic and antinociceptive activities of methanolic extract of the whole plant of Euphorbia thymifolia L., a plant used in folk medicine of Bangladesh for treatment of diabetes and pain.
METHODS: Antihyperglycemic activity studies were conducted in glucose-loaded mice by oral glucose tolerance tests. Mice were given various doses of the extract, followed by glucose (2 g/kg body weight), 1 h after administration of the extract. Serum glucose levels were measured 2 h after glucose administration. Antinociceptive activity studies were conducted in intraperitoneally acetic acid-injected mice through measurement of reductions in abdominal writhing times caused by acetic acid-induced gastric pain. Following a period of 1 h after oral administration of various doses of the extract, all mice received intraperitoneal injection of 1% acetic acid at a dose of 10 mL/kg body weight. To ensure bioavailability of acetic acid, a period of 5 min was given to each animal following which period the number of writhings was counted for 10 min.
RESULTS: The extract caused a significant dose-dependent reduction in serum glucose levels in mice, when administered at doses of 50, 100, 200 and 400 mg/kg body weight as compared to the control animals (P<0.05). The highest reduction of serum glucose (60.5%) was observed at a dose of 400 mg/kg. In comparison, a standard antihyperglycemic drug glibenclamide, when administered at a dose of 10 mg/kg body weight, lowered serum glucose levels by 48.6%. The extract also demonstrated a significant dose-dependent antinociceptive activity compared to control animals (P<0.05). At a dose of 400 mg/kg body weight, the number of abdominal writhings was inhibited by 40.9% as compared to 49.0% inhibition obtained with a standard antinociceptive drug aspirin, administered at a dose of 200 mg/kg body weight.
CONCLUSION: The significant antihyperglycemic and antinociceptive activities demonstrated by the extract validate the use of E. thymifolia in folk medicine of Bangladesh for treatment of diabetes and pain, and merit further scientific studies leading to discovery of efficacious drugs.
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Please cite this article as:
Rahmatullah M, Hasan SK, Ali Z, Rahman S, Jahan R. Antihyperglycemic and antinociceptive activities of methanolic extract of Euphorbia thymifolia L. whole plants. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(2): 228-232.
References:
1Nadkarni AK, Nadkarni KM. Dr. K. M. Nadkarni’s Indian materia medica. 3rd ed[M]. Mumbai: Popular Prakashan, 1982. 522-531.  
2Kirtikar KR, Basu BD. Indian medicinal plants. Volume 3. 2nd ed[M]. Dehradun: International Book Distributors, 1991. 2191-2208.  
3Khan NH, Rahman M, Nur-e-Kamal MS. Antibacterial activity of Euphorbia thymifolia Linn[J].Indian J Med Res, 1988, 87: 395-397.  
4Amaral ACF, Kuster RM, Gonalves JLS, Wigg MD. Antiviral investigation on the flavonoids of Chamaesyce thymifolia[J].Fitoterapia, 1999, 70(3): 293-295.  
5Lin CC, Cheng HY, Yang CM, Lin TC. Antioxidant and antiviral activities of Euphorbia thymifolia[J].J Biomed Sci, 2002, 9(6 Pt 2): 656-664.  
6Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC. Euphorbia thymifolia suppresses herpes simplex virus-2 infection by directly inactivating virus infectivity[J].Clin Exp Pharmacol Physiol, 2005, 32(5-6): 346-349.  
7Khan ZI, Nahar B, Jakaria MA, Rahman S, Chowdhury MH, Rahmatullah M. An evaluation of antihyperglycemic and antinociceptive effects of methanol extract of Cassia fistula L. (Fabaceae) leaves in Swiss albino mice[J].Adv Nat Appl Sci, 2010, 4(3): 305-310.  
8Rahman M, Siddika A, Bhadra B, Rahman S, Agarwala B, Chowdhury MH, Rahmatullah M. Antihyperglycemic activity studies on methanol extract of Petrea volubilis L. (Verbenaceae) leaves and Excoecaria agallocha L. (Euphorbiaceae) stems[J].Adv Nat Appl Sci, 2010, 4(3): 361-364.  
9Rahman M, Hasan N, Das AK, Hossain T, Jahan R, Khatun A, Rahmatullah M. Effect of Delonix regia leaf extract on glucose tolerance in glucose-induced hyperglycemic mice[J].Afr J Tradit Complement Altern Med, 2011, 8(1): 34-36.  
10Rahman MA, Bachar SC, Rahmatullah M. Analgesic and anti-inflammatory activity of methanolic extract of Acalypha indica Linn[J].Pak J Pharm Sci, 2010, 23(3): 256-258.  
11Joy KL, Kuttan R. Anti-diabetic activity of Picrorrhiza kurroa extract[J].J Ethnopharmacol, 1999, 67(2): 143-148.  
12Venkatesh S, Dayanand Reddy G, Reddy YS, Sathyavathy D, Madhava Reddy B. Effect of Helicteres isora root extracts on glucose tolerance in glucose-induced hyperglycemic rats[J].Fitoterapia, 2004, 75(3-4): 364-367.  
13Shanmugasundaram P, Venkataraman S. Anti-nociceptive activity of Hygrophila auriculata (Schum) Heine[J].Afr J Tradit Complement Altern Med, 2005, 2(1): 62-69.  
14Farjou IB, Al-Ani M, Guirgea SY. Lowering of blood glucose of diabetic rats by Artemisia extract[J].J Fac Med Baghdad, 1987, 92: 137-147.  
15Nyuna N, Njikam N, Abdennebi el H, Mbafor JT, Lamnaouer D. Hypoglycaemic and antihyperglycaemic activity of Ageratum conyzoides L. in rats[J].Afr J Tradit Complement Altern Med, 2009, 6(2): 123-130.  
16Bhowmik A, Khan LA, Akhter M, Rokeya B. Studies on the antidiabetic effects of Mangifera indica stem-barks and leaves on nondiabetic, type 1 and type 2 diabetic model rats[J].Bangladesh J Pharmacol, 2009, 4(2): 110-114.  
17Reynolds JEF, Prasad AB. Martindale: the extra pharmacopoeia. 28th ed[M]. London: The Pharmaceutical Press, 1982. 245.  
18Gilani AH, Rahman AU. Trends in ethnopharmacology[J].J Ethnopharmacol, 2005, 100(1-2): 43-49.  
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