Updated Saturday, July 21, 2018
 Journal Tools
RSS Feed
Sample Copy
Submit a Manuscript
Contact Us
Thanking Peer Reviewers
 Language Polishing
Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 4,  Pages: 416-423

DOI: 10.3736/jcim20120410
Original Experimental Research
Effects of electroacupuncture on expression of c-fos protein and N-methyl-D-aspartate receptor 1 in the rostral ventromedia medulla of rats with chronic visceral hyperalgesia
1. De-bo Qi (Laboratory of Neural Network and Systems Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China )
2. Wei-min Li (Laboratory of Neural Network and Systems Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China E-mail: E-mail: wmli9110@fudan.edu.cn)
OBJECTIVE: Acupuncture has been clinically proved to be effective in treating abdominal pain in patients with irritable bowel syndrome (IBS). However, its neurobiological mechanism remains largely unexplored. The aim of this study was to investigate the effect of electroacupuncture (EA) in relieving chronic visceral hyperalgesia and the possible involvement of N-methyl-D-aspartate receptor 1 (NR1) in rostral ventromedia medulla (RVM) of the brain in an IBS rat model.
METHODS: To establish the IBS rat model, male Sprague-Dawley neonatal rats received colon mechanical irritation on a daily basis from the 9th to the 22nd day after their birth. After a resting period of another two to four weeks, behavioral tests of pain threshold pressure (PTP) and abdominal withdrawal reflex (AWR) responding to colorectal distention (CRD) stimuli were conducted to judge the colorectal sensitive situation. Then administration of EA at acupoints of Zusanli (ST36) and Shangjuxu (ST37) bilaterally in the hind limbs was repeated four times every other day, while sham-EA was done by inserting needles at similar acupoints without electrical stimulation. Immunohistochemical method was used to display the expression of proto-oncogene protein c-fos and NR1 in RVM of rats.
RESULTS: The results demonstrated that the PTP values and AWR scores, in response to the CRD stimuli, significantly decreased and increased, respectively (P<0.01, P<0.01), while the number of immunoreactive neurons of c-fos protein and NR1 significantly increased in nucleus reticularis gigantocellularis (Gi), nucleus lateralis paragigantocellulari (LPGi), nucleus reticularis gigantocellularis pars alpha (GiA) and nucleus raphe magnus (NRM) of RVM in IBS model rats compared with the normal rats (P<0.05). After EA treatment, PTP values and AWR scores significantly increased and decreased, respectively (P<0.01, P<0.05); the number of immunoreactive neurons of c-fos and NR1 significantly decreased respectively in Gi, LPGi and GiA and in Gi, LPGi, GiA and NRM (P<0.05). No such effects on PTP values, AWR scores and the number of immunoreactive neurons of c-fos and NR1 were observed after sham-EA treatment.
CONCLUSION: These data provide the evidence that EA can relieve chronic visceral hyperalgesia in rats with IBS, and such an effect may be correlated with inhibitory modulation of hyperactivity of neurons by means of down-regulating the high expression of NR1 in RVM of IBS model rats.
Welcome to JIM! You are the number 16844 reader of this article!
Download Article:
[Full Text]      [PDF]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Qi DB, Li WM. Effects of electroacupuncture on expression of c-fos protein and N-methyl-D-aspartate receptor 1 in the rostral ventromedia medulla of rats with chronic visceral hyperalgesia. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(4): 416-423.
1Verne GN, Robinson ME, Price DD. Hypersensitivity to visceral and cutaneous pain in the irritable bowel syndrome[J].Pain, 2001, 93(1): 7-14.  
2Sengupta JN. Visceral pain: the neurophysiological mechanism[J].Handb Exp Pharmacol, 2009, (194): 31-74.  
3Hungin AP, Whorwell PJ, Tack J, Mearin F. The prevalence, patterns and impact of irritable bowel syndrome: an international survey of 40,000 subjects[J].Aliment Pharmacol Ther, 2003, 17(5): 643-650.  
4Gwee KA, Lu CL, Ghoshal UC. Epidemiology of irritable bowel syndrome in Asia: something old, something new, something borrowed[J].J Gastroenterol Hepatol, 2009, 24(10): 1601-1607.  
5Cash B, Sullivan S, Barghout V. Total costs of IBS: employer and managed care perspective[J].Am J Manag Care, 2005, 11(1 Suppl): S7-S16.  
6Talley NJ, Gabriel SE, Harmsen WS, Zinsmeister AR, Evans RW. Medical costs in community subjects with irritable bowel syndrome[J].Gastroenterology, 1995, 109(6): 1736-1741.  
7Schneider A, Weiland C, Enck P, Joos S, Streitberger K, Maser-Gluth C, Zipfel S, Bagheri S, Herzog W, Friederich HC. Neuroendocrinological effects of acupuncture treatment in patients with irritable bowel syndrome[J].Complement Ther Med, 2007, 15(4): 255-263.  
8Xiao WB, Liu YL. Rectal hypersensitivity reduced by acupoint TENS in patients with diarrhea-predominant irritable bowel syndrome: a pilot study[J].Dig Dis Sci, 2004, 49(2): 312-319.  
9Musial F, Tao I, Dobos G. Is the analgesic effect of acupuncture a placebo effect? Schmerz. 2009; 23(4): 341-346.
10Rohrbck RB, Hammer J, Vogelsang H, Talley NJ, Hammer HF. Acupuncture has a placebo effect on rectal perception but not on distensibility and spatial summation: a study in health and IBS[J].Am J Gastroenterol, 2004, 99(10): 1990-1997.  
11Zhuo M, Gebhart GF. Facilitation and attenuation of a visceral nociceptive reflex from the rostroventral medulla in the rat[J].Gastroenterology, 2002, 122(4): 1007-1019.  
12Zhuo M, Sengupta JN, Gebhart GF. Biphasic modulation of spinal visceral nociceptive transmission from the rostroventral medial medulla in the rat[J].J Neurophysiol, 2002, 87(5): 2225-2236.  
13Urban MO, Coutinho SV, Gebhart GF. Biphasic modulation of visceral nociception by neurotensin in rat rostral ventromedial medulla[J].J Pharmacol Exp Ther, 1999, 290(1): 207-213.  
14Coutinho SV, Urban MO, Gebhart GF. Role of glutamate receptors and nitric oxide in the rostral ventromedial medulla in visceral hyperalgesia[J].Pain, 1998, 78(1): 59-69.  
15Coutinho SV, Urban MO, Gebhart GF. The role of CNS NMDA receptors and nitric oxide in visceral hyperalgesia[J].Eur J Pharmacol, 2001, 429(1-3): 319-325.  
16Porreca F, Ossipov MH, Gebhart GF. Chronic pain and medullary descending facilitation[J].Trends Neurosci, 2002, 25(6): 319-325.  
17Al-Chaer ED, Kawasaki M, Pasricha PJ. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development[J].Gastroenterology, 2000, 119(5): 1276-1285.  
18Cui KM, Li WM, Gao X, Chung K, Chung JM, Wu GC. Electro-acupuncture relieves chronic visceral hyperalgesia in rats[J].Neurosci Lett, 2005, 376(1): 20-23.  
19Zhou J, Li WM. Influence of electroacupuncture on expression of N-methyl-D-aspartatic receptors in the spinal cord of irritable bowel syndrome rat. Shanghai Zhen Jiu Za Zhi. 2008; 27(6): 38-40. Chinese with abstract in English.
20Sagar SM, Sharp FR, Curran T. Expression of c-fos protein in brain: metabolic mapping at the cellular level[J].Science, 1988, 240(4857): 1328-1331.  
21Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 6th ed[M]. Waltham: Academic Press, 2007. 270-298.  
22Drossman DA. Chronic functional abdominal pain[J].Am J Gastroenterol, 1996, 91(11): 2270-2281.  
23Winston J, Shenoy M, Medley D, Naniwadekar A, Pasricha PJ. The vanilloid receptor initiates and maintains colonic hypersensitivity induced by neonatal colon irritation in rats[J].Gastroenterology, 2007, 132(2): 615-627.  
24Chung EK, Bian ZX, Xu HX, Sung JJ. Neonatal maternal separation increases brain-derived neurotrophic factor and tyrosine kinase receptor B expression in the descending pain modulatory system[J].Neurosignals, 2009, 17(3): 213-221.  
 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号