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Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 4,  Pages: 430-435

DOI: 10.3736/jcim20120412
Original Experimental Research
Antitumor activity of ethanol extract of Gracilaria edulis (Gmelin) Silva on Ehrlich ascites carcinoma-bearing mice
1. Meenakshi Sundaram (Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India )
2. Satyajit Patra (School of Medicine, University of Alabama, Birmingham, Alabama 35294, USA E-mail: E-mail: spatra@uab.edu)
3. Gunasingham Maniarasu (Department of Biotechnology, Srimad Andavan Arts and Science College, Trichy 620005, Tamilnadu, India )
OBJECTIVE: To evaluate antitumor activity of Gracilaria edulis in Swiss albino mice with Ehrlich ascites carcinoma (EAC).
METHODS: Tumors were induced in mice by intraperitoneal injection of EAC cells. Ethanol extract of Gracilaria edulis (EEGE) was administered to the experimental animals in different doses after 24 h of tumor inoculation. The antitumor effect of the EEGE was evaluated by assessing in vitro cytotoxicity, survival time, biochemical parameters and hepatic enzyme levels.
RESULTS: EEGE increased the life span of EAC-bearing mice compared with that of the model control mice (P<0.05 or P<0.01). EEGE treatment also converted the changes of biochemical parameters and hepatic enzyme levels in the EAC-bearing mice (P<0.05 or P<0.01). EEGE induced inhibition of tumor formation in EAC-bearing mice compared with that of the model control group (P<0.05 or P<0.01).
CONCLUSION: The present study scientifically proved the antitumor activity of marine algae G. edulis and the effect can be correlated with doses.
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Please cite this article as:
Sundaram M, Patra S, Maniarasu G. Antitumor activity of ethanol extract of Gracilaria edulis (Gmelin) Silva on Ehrlich ascites carcinoma-bearing mice. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(4): 430-435.
References:
1Garcia M, Jemal A, Ward EM, Center MM, Hao Y, Siegel RL, Thun MJ. Global cancer facts & figures 2007. Atlanta, GA: American Cancer Society. 2007.
2Tongyoo A. Targeted therapy: novel agents against cancer[J].J Med Assoc Thai, 2010, 93(Suppl 7): S311-S323.  
3Guarino M, Rubino B, Ballabio G. The role of epithelial-mesenchymal transition in cancer pathology[J].Pathology, 2007, 39(3): 305-318.  
4Kurkjian C, Kummar S. Advances in the treatment of metastatic colorectal cancer[J].Am J Ther, 2009, 16(5): 412-420.  
5Balunas MJ, Su B, Brueggemeier RW, Kinghorn AD. Natural products as aromatase inhibitors[J].Anticancer Agents Med Chem, 2008, 8(6): 646-682.  
6Blunt JW, Copp BR, Munro MH, Northcote PT, Prinsep MR. Marine natural products[J].Nat Prod Rep, 2003, 20(1): 1-48.  
7Dholwani KK, Saluja AK, Gupta AR, Shah DR. A review on plant-derived natural products and their analogs with anti-tumor activity[J].Indian J Pharmacol, 2008, 40(2): 49-58.  
8Wagner H, Bladt S, Zgainski EM. Plant drug analysis: a thin layer chromatography atlas[M]. New York: Springer-Verlag, 1984. 298.  
9Noaman E, Badr El-Din NK, Bibars MA, Abou Mossallam AA, Ghoneum M. Antioxidant potential by arabinoxylan rice bran, MGN-3/biobran, represents a mechanism for its oncostatic effect against murine solid Ehrlich carcinoma[J].Cancer Lett, 2008, 268(2): 348-359.  
10Organization of Economic Co-operation and Development. Revised draft guidelines 425, OECD Guideline for the testing of chemicals. (2001-12-17) [2011-09-10]. http://www.oecd.org/dataoecd/17/51/1948378.pdf.
11Zhivotosky B, Orrenius S. Assessment of apoptosis and necrosis by DNA fragmentation and morphological criteria. Curr Protoc Cell Biol. 2001; Chapter 18: Unit 18.3.
12Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability[J].J Immunol Methods, 1986, 89(2): 271-277.  
13Sur P, Ganguly DK. Tea plant root extract (TRE) as an antineoplastic agent[J].Planta Med, 1994, 60(2): 106-109.  
14Fossati P, Prencipe L, Berti G. Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine[J].Clin Chem, 1980, 26(2): 227-231.  
15Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent[J].J Biol Chem, 1951, 193(1): 265-275.  
16Richardson RW, Setchell KD, Woodman DD. An improved procedure for the estimation of serum cholesterol[J].Clin Chim Acta, 1971, 31(2): 403-407.  
17Mohun AF, Cook IJ. Simple methods for measuring serum levels of the glutamic-oxalacetic and glutamic-pyruvic transaminases in routine laboratories[J].J Clin Pathol, 1957, 10(4): 394-399.  
18Devi GK, Manivannan K, Thirumaran G, Rajathi FA, Anantharaman P. In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pac J Trop Med. 2011; 4(3): 205-211.
19Ganesan P, Kumar CS, Bhaskar N. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds[J].Bioresour Technol, 2008, 99(8): 2717-2723.  
20Natarajan S, Shanmugiahthevar KP, Kasi PD. Cholinesterase inhibitors from Sargassum and Gracilaria gracilis: seaweeds inhabiting South Indian coastal areas (Hare Island, Gulf of Mannar)[J].Nat Prod Res, 2009, 23(4): 355-369.  
21Zhou YH, Ragan MA. Characterization of the polyubiquitin gene in the marine red alga Gracilaria verrucosa[J].Biochim Biophys Acta, 1995, 1261(2): 215-222.  
22Nakajima K, Yokoyama A, Nakajima Y. Anticancer effects of a tertiary sulfonium compound, dimethylsulfoniopropionate, in green sea algae on Ehrlich ascites carcinoma-bearing mice[J].J Nutr Sci Vitaminol (Tokyo), 2009, 55(5): 434-438.  
23DeCosse JJ, Cennerazzo WJ. Quality-of-life management of patients with colorectal cancer[J].CA Cancer J Clin, 1997, 47(4): 198-206.  
24Badary OA. Thymoquinone attenuates ifosfamide-induced Fanconi syndrome in rats and enhances its antitumor activity in mice[J].J Ethnopharmacol, 1999, 67(2): 135-142.  
25Daoud SS, Fetouh MI, Giovanella BC. Antitumor effect of liposome-incorporated camptothecin in human malignant xenografts[J].Anticancer Drugs, 1995, 6(1): 83-93.  
26Huot J, Roy G, Lambert H, Chrétien P, Landry J. Increased survival after treatments with anticancer agents of Chinese hamster cells expressing the human Mr 27,000 heat shock protein[J].Cancer Res, 1991, 51(19): 5245-5252.  
27Reinhold HS, van den Berg-Blok AE, van den Berg AP. Dose-effect relationships for glucose-induced tumour acidification and its erythrocyte flux[J].Eur J Cancer, 1991, 27(9): 1151-1154.  
28Bartrons R, Caro J. Hypoxia, glucose metabolism and the Warburg’s effect[J].J Bioenerg Biomembr, 2007, 39(3): 223-229.  
29Metwally NS, Ali SA, Mohamed AM, Khaled HM, Ahmed SA. Levels of certain tumor markers as differential factors between bilharzial and non-biharzial bladder cancer among Egyptian patients[J].Cancer Cell Int, 2011, 11(1): 8.  
30Ramadori G, Lenzi M, Dienes HP, Meyer zum Büschenfelde KH. Binding properties of mechanically and enzymatically isolated hepatocytes for IgG and C3[J].Liver, 1983, 3(6): 358-368.  
31Pizzuti GP, Salvatori GC. Some blood parameters of water buffalo in different physiological conditions[J].Boll Soc Ital Biol Sper, 1993, 69(10): 649-654.  
32Chakraborty T, Chatterjee A, Saralaya MG, Chatterjee M. Chemopreventive effect of vanadium in a rodent model of chemical hepatocarcinogenesis: reflections in oxidative DNA damage, energy-dispersive X-ray fluorescence profile and metallothionein expression[J].J Biol Inorg Chem, 2006, 11(7): 855-866.  
33Adeyemi OO, Okpo SO, Okpaka O. The analgesic effect of the methanolic extract of Acanthus montanus[J].J Ethnopharmacol, 2004, 90(1): 45-48.  
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