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Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 4,  Pages: 448-453

DOI: 10.3736/jcim20120414
Original Experimental Research
Effects of Chinese herbal medicine Feiyanning decoction on expressions of nucleosome conformation-regulating factors H3-K56, Rtt109, Asf1 and E2F1 in Lewis-bearing mice
1. Zhan Zheng (Institute of Traditional Chinese Medicine Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China E-mail: E-mail: zhengzhan2005@sina.com)
2. Ju-yong Wang (Institute of Traditional Chinese Medicine Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
3. Qing Wang (Institute of Traditional Chinese Medicine Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
4. Ling Xu (Institute of Traditional Chinese Medicine Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
5. Zhen-ye Xu (Institute of Traditional Chinese Medicine Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
OBJECTIVE: Malignant tumor cells were found with an abnormal cell cycle. Previous in vivo experiment had confirmed the Feiyanning’s intervention effect on checkpoint signaling of G1/S in the cell cycle. This study was to further observe the expressions of nucleosome conformation-regulating factors intervened by Feiyanning decoction in S phase.
METHODS: Lewis lung carcinoma models of C57BL/6 mice were established. Sixty mice were randomly divided into four groups: normal control group, model control group, Feiyanning group, and cisplatin group. There were 15 mice in each group. Tumor weight and tumor inhibition rate were observed. In addition, the cell cycle distribution was detected by flow cytometry and the proliferation index was calculated. Furthermore, mRNA and protein expressions of H3-K56, regulator of Ty1 transposition 109 (Rtt109), antisilencing function 1 (Asf1) and E2F1 were analyzed by real-time polymerase chain reaction and Western blot methods, respectively.
RESULTS: The tumor weights of mice in the Feiyanning group and the cisplatin group were lower than those in the model group (P<0.01), with tumor inhibition rates of 27.92% and 42.50%, respectively. Cancer cell proliferation index and proportion of cancer cell population in S phase in the Feiyanning group were significantly lower than those in the cisplatin group (P<0.01). The mRNA and protein levels of H3-K56, Rtt109, Asf1, E2F1 in the Feiyanning group were lower than those in the model group (P<0.05).
CONCLUSION: Feiyanning plays a role in intervening in the abnormal cell cycle by nucleosome conformation-regulating factors and thus inhibits the lung cancer cell proliferation.
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Please cite this article as:
Zheng Z, Wang JY, Wang Q, Xu L, Xu ZY. Effects of Chinese herbal medicine Feiyanning decoction on expressions of nucleosome conformation-regulating factors H3-K56, Rtt109, Asf1 and E2F1 in Lewis-bearing mice. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(4): 448-453.
References:
1Hung RJ, Baragatti M, Thomas D, McKay J, Szeszenia-Dabrowska N, Zaridze D, Lissowska J, Rudnai P, Fabianova E, Mates D, Foretova L, Janout V, Bencko V, Chabrier A, Moullan N, Canzian F, Hall J, Boffetta P, Brennan P. Inherited predisposition of lung cancer: a hierarchical modeling approach to DNA repair and cell cycle control pathways[J].Cancer Epidemiol Biomarkers Prev, 2007, 16(12): 2736-2744.  
2Xu ZY, Jin CJ, Zhou CC, Wang ZQ, Zhou WD, Deng HB, Zhang M, Su W, Cai XY. Treatment of advanced non-small-cell lung cancer with Chinese herbal medicine by stages combined with chemotherapy[J].J Cancer Res Clin Oncol, 2011, 137(7): 1117-1122.  
3Wang JY, Zheng Z, Xu ZY, Guo J, Su W. Effects of Feiyanning Decoction on proliferation of lung adenocarcinoma cell line and their production of interleukin-6 and interleukin-8 induced by tumor necrosis factor-α[J]. J Chin Integr Med, 2009, 7(8): 741-745. Chinese
4Zhang M, Xu ZY, Bai B, Dong Y. Feiyanning Decoction down-regulates the expressions of phosphorylated Akt and hypoxia inducible factor-1α proteins in transplanted lung adenocarcinoma in nude mice[J]. J Chin Integr Med, 2008, 6(6): 616-620. Chinese with abstract in English
5Zheng Z, Wang JY, Xu ZY. Effect of Feiyanning granule in antagonizing Lewis lung cancer cell proliferation through cell cycle G1/S checkpoint dominating signaling intervention. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009; 29(11): 1018-1022. Chinese with abstract in English.
6Zhang DF. Experiments of pharmacology and traditional Chinese medicine pharmacology[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2002. 8-10. Chinese
7Han J, Zhou H, Horazdovsky B, Zhang KL, Xu RM, Zhang ZG. Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication[J].Science, 2007, 315(5812): 653-655.  
8Williams SK, Truong D, Tyler JK. Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation[J].Proc Natl Acad Sci U S A, 2008, 105(26): 9000-9005.  
9Miller A, Yang B, Foster T, Kirchmaier AL. Proliferating cell nuclear antigen and ASF1 modulate silent chromatin in Saccharomyces cerevisiae via lysine 56 on histone H3[J].Genetics, 2008, 179(2): 793-809.  
10English CM, Adkins MW, Carson JJ, Churchill ME, Tyler JK. Structural basis for the histone chaperone activity of Asf1[J].Cell, 2006, 127(3): 458-460.  
11Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T. Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4[J].Nature, 2007, 446(7133): 338-341.  
12Groth A, Corpet A, Cook AJ, Roche D, Bartek J, Lukas J, Almouzni G. Regulation of replication fork progression through histone supply and demand[J].Science, 2007, 318(5858): 1928-1931.  
13Hayashi R, Goto Y, Tanaka R, Oonogi K, Hisasue M, Yoshida K. Transcriptional regulation of human chromatin assembly factor ASF1[J].DNA Cell Biol, 2007, 26(2): 91-99.  
14Hallstrom TC, Mori S, Nevins JR. An E2F1-dependent gene expression program that determines the balance between proliferation and cell death[J].Cancer Cell, 2008, 13(1): 11-12.  
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