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Journal of Integrative Medicine: Volume 11, 2013   Issue 4,  Pages: 253-261

DOI: 10.3736/jintegrmed2013025
Research Article
Pharmacological evaluation of Musa seminifera Lour. fruit
1. Sanjib Saha (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh E-mail: sanjibsaha1991@yahoo.com)
2. Faroque Hossain (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
3. Md. Anisuzzman (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
4. Md. Khirul Islam (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )

OBJECTIVE: To study potential antioxidant, analgesic, antidiarrheal, and antibacterial activities of the ethanol extract of Musa seminifera Lour. fruit in different established in vivo and in vitro experimental models.

METHODS: In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was determined using Folin-Ciocalteu’s reagent. Reducing ability was evaluated by ferric reducing power assay. Peripherally and centrally acting analgesic activity was studied in three different in vivo models, namely, acetic acid-induced writhing, hot-plate test, and tail-flick test in Swiss albino mice. In vivo antidiarrheal activity was evaluated in castor oil- and magnesium sulfate-induced diarrhea in mice. Gastrointestinal motility test was also carried out in mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was assessed by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Acute toxicity test was conducted to assess the safe doses of the extract.

RESULTS: The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radical-scavenging assay. Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g of dried fruits extract. Reducing power was in a concentration-dependent manner, and strongly comparable with the standard ascorbic acid. The extract demonstrated significant inhibition of writhing in acetic acid-induced writhing test at both dose levels (P<0.01). The extract also raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner, and the results were statistically significant (P<0.01). The extract significantly (P<0.01) increased latent period, and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also decreased gastrointestinal motility in mice. In disk diffusion assay, the extract showed potential antibacterial activity against all the tested bacterial strains.

CONCLUSION: The results suggest that the ethanol extract of M. seminifera fruit has potential antioxidant, analgesic, antidiarrheal, and antibacterial activities.

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Saha S, Hossain F, Anisuzzman M, Islam MK. Pharmacological evaluation of Musa seminifera Lour. fruit. J Integr Med. 2013; 11(4): 253-261.
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