Updated Saturday, April 21, 2018
 Journal Tools
RSS Feed
Sample Copy
Submit a Manuscript
Contact Us
Subscription
Advertising
Thanking Peer Reviewers
 Language Polishing
Journal of Integrative Medicine: Volume 11, 2013   Issue 5,  Pages: 343-351

DOI: 10.3736/jintegrmed2013045
Research Article
Preliminary pharmacological evaluation of Alocasia indica Schott tuber
1. Md. Khirul Islam (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh E-mail: khairul08ku@gmail.com)
2. Imran Mahmud (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
3. Sanjib Saha (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
4. Asit Baron Sarker (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
5. Himangsu Mondal (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
6. A. S. M. Monjur-Al-Hossain (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
7. Md. Anisuzzman (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )

OBJECTIVE: To elucidate potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activities of the ethanol extract of Alocasia indica Schott tuber in different experimental models established in vitro and in vivo.

METHODS: In vitro antioxidant activity was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was estimated by using Folin-Ciocalteu’s reagent while reducing ability was measured by ferric reducing power assay. In vivo antidiarrheal studies were carried out in mice, and the activity was evaluated in castor oil and magnesium sulfate-induced diarrhea. Disk diffusion assay was utilized to determine antibacterial activity against a number of pathogenic bacterial strains. Acute toxicity test was carried out to measure the safe doses for the extract.

RESULTS: In DPPH radical-scavenging assay, the extract exhibited strong radical-scavenging activity with the 50% inhibitory concentration value of 42.66 μg/mL. Total phenolic content was found to be 542.26 mg gallic acid equivalent per 100 g of dried tuber extract, whereas flavonoid content was found to be 4.30 mg quercetin equivalent/g of dried tuber extract. In reducing power assay, the extract showed strong reducing power in a concentration-dependent manner. The extract significantly (P < 0.01) enhanced the latent period and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also lessened gastrointestinal motility in mice. Potential antibacterial activity was exhibited by the extract against all the tested bacterial strains in disk diffusion assay. The 50% lethal concentration against brine shrimp nauplii was 81.09 μg/mL.

CONCLUSION: The results demonstrated that the ethanol extract of A. indica has potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activity.

Welcome to JIM! You are the number 40547 reader of this article!
Download Article:
[Full Text]      [PDF]      [Previous]      [Next]      [This Issue]
Please cite this article as:
Islam MK, Mahmud I, Saha S, Sarker AB, Mondal H, Monjur-Al-Hossain ASM, Anisuzzman M. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. J Integr Med. 2013; 11(5): 343-351.
References:
1Ramawat KG, Dass S, Mathur M. The chemical diversity of bioactive molecules and therapeutic potential of medicinal plants. In: Ramawat KG. Herbal drugs: ethnomedicine to modern medicine[M]. Berlin: Springer, 2009. 420.  
2Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum[J].Sci Res Essays, 2007, 2(5): 163-166.  
3Rahman MM, Hossain MA, Siddique SA, Biplab KP, Uddin MH. Antihyperglycemic, antioxidant, and cytotoxic activities of Alocasia macrorrhizos (L.) rhizome extract[J].Turk J Biol, 2012, 36: 574-579.  
4Kirtikar KR, Basu BD. Alocasia indica in Indian medicinal plants. 2nd ed[M]. Dehradun: New Cannaught Place, 1975. 2617.  
5Mulla WA, Thorat VS, Patil RV, Burade KB. Anthelmintic activity of leaves of Alocasia indica Linn[J].Int J PharmTech Res, 2010, 2(1): 26-30.  
6Rahmatullah M, Ferdausi D, Mollik AH, Jahan R, Chowdhury MH, Haque WM. A survey of medicinal plants used by Kavirajes of Chalna area, Khulna district, Bangladesh[J].Afr J Tradit Complement Altern Med, 2009, 7(2): 91-97.  
7Prajapati ND. A handbook of medicinal plants[M]. Jodhpur: Agrobios India, 2003. 32.  
8Mulla W, Kuchekar S, Thorat V, Chopade A, Kuchekar B. Antioxidant, antinociceptive and anti-inflammatory activities of ethanolic extract of leaves of Alocasia indica (Schott.)[J].J Young Pharm, 2010, 2(2): 137-143.  
9Patil SH, Sreenivas SA, Deshmukh PV, Srikanth M, Choudhury A, Wagh AE. Antidiabetic and hypolipidemic potential of Alocasia indica Schott. leaves in streptozotocin induced diabetic rats[J].Int J Drug Dev Res, 2012, 4: 368-374.  
10Bhatt SK, Saxena VR. Antifungal activity of seeds of extract of Alocasia indica[J].Indian Drugs, 1980, 17: 210.  
11Mulla WA, Salunkhe VR, Bhise SB. Hepatoprotective activity of hydroalcoholic extract of leaves of Alocasia indica (Linn.)[J].Indian J Exp Biol, 2009, 47(10): 816-821.  
12Vijapur S. Anthelmintic activity of Alocasia indica Schott. rootstocks[J].Int J Drug Dev Res, 2012, 4: 211-214.  
13Sharma OP, Bhat TK. DPPH antioxidant assay revisited[J].Food Chem, 2009, 113(4): 1202-1205.  
14Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities[J].Chem Pharm Bull, 2003, 51(5): 595-598.  
15Talukder C, Saha S, Adhikari S, Mondal HK, Islam MK, Anisuzzman M. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Flacourtia jangomas (Lour.) Raeusch. leaves[J].Pharmacologyonline, 2012, 3: 20-28.  
16Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables[J].J Univ Chem Technol Metall, 2005, 40: 255-260.  
17Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam)[J].Int Food Res J, 2010, 17: 97-106.  
18Oyaizu M. Studies on product of browning reaction prepared from glucosamine[J].Japn J Nutri, 1986, 44: 307-315.  
19Lorke D. A new approach to practical acute toxicity testing[J].Arch Toxicol, 1983, 54(4): 275-287.  
20Abdullahi AL, Agho MO, Amos S, Gamaniel KS, Wambebe C. Antidiarrhoeal activity of the aqueous extract of Terminalia avicennoides roots[J].Phytother Res, 2001, 15(5): 431-434.  
21Adeyemi OO, Akindele AJ, Ogunleye EA. Evaluation of the antidiarrhoeal effect of Sanseviera liberica Gerome & Labroy (Agavaceae) root extract[J].J Ethnopharmacol, 2009, 123(3): 459-463.  
22Shilpi JA, Taufiq-Ur-Rahman M, Uddin SJ, Alam MS, Sadhu SK, Seidel V. Preliminary pharmacological screening of Bixa orellana L. leaves[J].J Ethnopharmacol, 2006, 108(2): 264-271.  
23Doherty NS. Inhibition of arachidonic acid release, mechanism by which glucocorticoids inhibit endotoxin-induced diarrhea[J].Br J Pharmacol, 1981, 73(2): 549-554.  
24Saha S, Islam MK, Anisuzzman M, Hasan MM, Hossain F, Talukder C. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Phoenix paludosa Roxb. leaves[J].Int J Basic Med Sci Pharm, 2012, 2(2): 46-52.  
25Saha S, Hossain F, Anisuzzman M, Islam MK. Pharmacological evaluation of Musa seminifera Lour. fruit[J]. J Integr Med, 2013, 11(4): 253-261.  
26Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method[J].Am J Clin Pathol, 1966, 45(4): 493-496.  
27Saha S, Anisuzzman M, Islam MK, Mondal H, Talukder C. Antibacterial and cytotoxic potential of Dalbergia spinosa Roxb. leaves[J].Int J Pharm Sci Res, 2013, 4(1): 512-515.  
28Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents[J].Planta Med, 1982, 45(5): 31-34.  
29Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale)[J].Food Chem, 2008, 111(1): 38-44.  
30Paixao N, Perestrelo R, Marques JC, Camara JS. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines[J].Food Chem, 2007, 105(1): 204-214.  
31Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions[J].Food Chem, 2003, 83(4): 547-550.  
32Li HY, Hao ZB, Wang XL, Huang L, Li JP. Antioxidant activities of extracts and fractions from Lysimachia foenumgraecum Hance[J].Bioresour Technol, 2009, 100(2): 970-974.  
33Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves[J].J Agric Food Chem, 1995, 43(7): 1813-1817.  
34Ribeiro SMR, Barbosa LCA, Queiroz J, Knodler HM, Schieber A. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties[J].Food Chem, 2008, 110(3): 620-626.  
35Ammon HV, Thomas PJ, Phillips SF. Effects of oleic and ricinoleic acids on net jejunal water and electrolyte movement. Perfusion studies in man[J].J Clin Invest, 1974, 53(2): 374-379.  
36Zavala MA, Pérez S, Pérez C, Vargas R, Pérez RM. Antidiarrhoeal activity of Waltheria americana, Commelina coelestis and Alternanthera repens[J].J Ethnopharmacol, 1998, 61(1): 41-47.  
37Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition[J].Free Radic Res, 2006, 40(2): 223-231.  
38Borowitz JL, McLaughlin JL. Evidence for calcium channels in brine shrimp: diltiazem protects shrimp against cadmium[J].Bull Environ Contam Toxicol, 1992, 48(3): 435-440.  
39Silva TMS, Nascimento RJB, Batista MM, Agra MF, Camara CA. Brine shrimp bioassay of some species of Solanum from Northestern Brazil[J].Brazil J Pharmacol, 2007, 17(1): 35-38.  
40Anderson JE, Goetz CM, McLaughlin JL, Suffness M. A blind comparison of simple bench-top bioassays and human tumor cell cytotoxicities as antitumor prescreens[J].Phytochem Anal, 1991, 2(3): 107-111.  
 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号