Updated Wednesday, May 22, 2019
 Journal Tools
RSS Feed
Sample Copy
Submit a Manuscript
Contact Us
Subscription
Advertising
Thanking Peer Reviewers
 Language Polishing
Journal of Integrative Medicine: Volume 14, 2016   Issue 6,  Pages: 473–479

DOI: 10.1016/S2095-4964(16)60272-8
Research Article
Chyawanprash, a formulation of traditional Ayurvedic medicine, shows a protective effect on skin photoaging in hairless mice
1. Yuki Takauji (Graduate School of Nanobioscience, Yokohama City University, Kanagawa 236-0027, Japan )
2. Kyoko Morino (Graduate School of Nanobioscience, Yokohama City University, Kanagawa 236-0027, Japan )
3. Kensuke Miki (Graduate School of Nanobioscience, Yokohama City University, Kanagawa 236-0027, Japan )
4. Mohammad Hossain (Department of Biochemistry, Primeasia University, Dhaka 1213, Bangladesh )
5. Dai Ayusawa (Graduate School of Nanobioscience, Yokohama City University, Kanagawa 236-0027, Japan )
6. Michihiko Fujii (Graduate School of Nanobioscience, Yokohama City University, Kanagawa 236-0027, Japan )

ABSTRACT

OBJECTIVE: Chronic exposure to ultraviolet (UV) radiation induces skin photoaging (premature skin aging). UV irradiation generates reactive oxygen species (ROS), which are shown to play a pivotal role in skin photoaging. Ayurveda is a holistic traditional medical system, and Chyawanprash is one of the most popular formulations in Ayurveda. Since maintenance of the function and appearance of skin is important, we examined whether Chyawanprash has a protective effect on skin photoaging.

METHODS: To examine the effect of Chyawanprash on skin photoaging, hairless mice were administered with Chyawanprash in drinking water for 3 weeks, and then repeatedly exposed to ultraviolet light B (UVB) irradiation (225 or 450 mJ/cm2) to induce skin photoaging. To further examine the function of Chyawanprash, its effects were examined in cells cultured in vitro. Chyawanprash was added in culture medium, and examined for the effect on the growth of human keratinocytes, and for the ability to eliminate ROS which generated by paraquat (50 μmol/L) in HeLa cells.

RESULTS: UVB irradiation caused symptoms such as rough skin, erythema, and edema on the skin in hairless mice, but administration of Chyawanprash relieved these symptoms. Further, Chyawanprash significantly suppressed epidermal thickening, a typical marker of skin photoaging, in mice. We then analyzed the effect of Chyawanprash in human cells in culture, and found that Chyawanprash enhanced the growth of human keratinocytes, and efficiently eliminated ROS, which are causally involved in skin photoaging, in HeLa cells.

CONCLUSION: These findings suggested that Chyawanprash may have beneficial effects on slowing skin photoaging.

Welcome to JIM! You are the number 3781 reader of this article!
Download Article:
[Full Text]      [PDF]      [Next]      [This Issue]
Please cite this article as:
Takauji Y, Morino K, Miki K, Hossain M, Ayusawa D, Fujii M. Chyawanprash, a formulation of traditional Ayurvedic medicine, shows a protective effect on skin photoaging in hairless mice. J Integr Med. 2016; 14(6): 473–479.
References:
1Chaudhary A, Singh N. Contribution of world health organization in the global acceptance of Ayurveda[J].J Ayurveda Integr Med, 2011, 2(4): 179–186.  
2Parle M, Bansal N. Traditional medicinal formulation, Chyawanprash—a review[J].Indian J Tradit Know, 2006, 5(4): 484–488.  
3Kumar A, Rinwa P, Kaur P. Chyawanprash: a wonder Indian Rasayana from Ayurveda to Modern Age[J].Critical Rev Pharm S, 2012, 1(2): 1–8.  
4Madaan A, Kanjilal S, Gupta A, Sastry JL, Verma R, Singh AT, Jaggi M. Evaluation of immunostimulatory activity of Chyawanprash using in vitro assays[J].Indian J Exp Biol, 2015, 53(3): 158–163.  
5Jose JK, Kuttan R. Hepatoprotective activity of Emblica officinalis and Chyavanaprash[J].J Ethnopharmacol, 2000, 72(1–2): 135–140.  
6Sur TK, Pandit S, Mukherjee R, Pramanik T, Debnath PK, Bandyopadhyay SK, Bhattacharyya D. Effect of Sonachandi Chyawanprash and Chyawanprash Plus—two herbal formulations on immunomodulation[J].Nepal Med Coll J, 2004, 6(2): 126–128.  
7Bansal N, Parle M. Beneficial effect of chyawanprash on cognitive function in aged mice[J].Pharm Biol, 2011, 49(1): 2–8.  
8Parle M, Bansal N. Antiamnesic activity of an ayurvedic formulation chyawanprash in mice[J].Evid Based Complement Alternat Med, 2011, 2011: 898593.  
9Manjunatha S, Jaryal AK, Bijlani RL, Sachdeva U, Gupta SK. Effect of Chyawanprash and vitamin C on glucose tolerance and lipoprotein profile[J].Indian J Physiol Pharmacol, 2001, 45(1): 71–79.  
10Jose JK, Kuttan R. Antioxidant activity of Emblica officinalis[J].J Clin Biochem Nutr, 1995, 19(2): 63–70.  
11Jose JK, Kuttan G, George J, Kuttan R. Antimutagenic and anticarcinogenic activity of Emblica officinalis Gaertn[J].J Clin Biochem Nutr, 1997, 22(3): 171–176.  
12Jose JK, Kuttan G, Kuttan R. Antitumour activity of Emblica officinalis[J].J Ethnopharmacol, 2001, 75(2–3): 65–69.  
13Kligman LH, Kligman AM. The nature of photoaging: its prevention and repair[J].Photodermatol, 1986, 3(4): 215–227.  
14Rittie L, Fisher GJ. UV-light-induced signal cascades and skin aging[J].Ageing Res Rev, 2002, 1(4): 705–720.  
15Pandel R, Poljsak B, Godic A, Dahmane R. Skin photoaging and the role of antioxidants in its prevention[J].ISRN Dermatol, 2013, 2013: 930164.  
16Sumiyoshi M, Kimura Y. Effects of a turmeric extract (Curcuma longa) on chronic ultraviolet B irradiation-induced skin damage in melanin-possessing hairless mice[J].Phytomedicine, 2009, 16(12): 1137–1143.  
17Ham SA, Kang ES, Yoo T, Lim HH, Lee WJ, Hwang JS, Paek KS, Seo HG. Dalbergia odorifera extract ameliorates UVB-induced wrinkle formation by modulating expression of extracellular matrix proteins. Drug Dev Res. 2015. Epub ahead of print.
18Jimbo N, Kawada C, Nomura Y. Herb extracts and collagen hydrolysate improve skin damage resulting from ultraviolet-induced aging in hairless mice[J].Biosci Biotechnol Biochem, 2015, 79(10): 1624–1628.  
19Kang TH, Park HM, Kim YB, Kim H, Kim N, Do JH, Kang C, Cho Y, Kim SY. Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice[J].J Ethnopharmacol, 2009, 123(3): 446–451.  
20Park JE, Pyun HB, Woo SW, Jeong JH, Hwang JK. The protective effect of Kaempferia parviflora extract on UVB-induced skin photoaging in hairless mice[J].Photodermatol Photoimmunol Photomed, 2014, 30(5): 237–245.  
21Hwang E, Park SY, Lee HJ, Sun ZW, Lee TY, Song HG, Shin HS, Yi TH. Vigna angularis water extracts protect against ultraviolet B-exposed skin aging in vitro and in vivo[J].J Med Food, 2014, 17(12): 1339–1349.  
22Hwang E, Lee DG, Park SH, Oh MS, Kim SY. Coriander leaf extract exerts antioxidant activity and protects against UVB-induced photoaging of skin by regulation of procollagen type I and MMP-1 expression[J].J Med Food, 2014, 17(9): 985–995.  
23Song JH, Bae EY, Choi G, Hyun JW, Lee MY, Lee HW, Chae S. Protective effect of mango (Mangifera indica L.) against UVB-induced skin aging in hairless mice[J].Photodermatol Photoimmunol Photomed, 2013, 29(2): 84–89.  
24Im AR, Song JH, Lee MY, Yeon SH, Um KA, Chae S. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice[J].BMC Complement Altern Med, 2014, 14: 424.  
25Lim JY, Kim OK, Lee J, Lee MJ, Kang N, Hwang JK. Protective effect of the standardized green tea seed extract on UVB-induced skin photoaging in hairless mice[J].Nutr Res Pract, 2014, 8(4): 398–403.  
26Ayurvedic Formulary Committee of Bangladesh. Bangladesh national formulary of ayurvedic medicine 2011. 2nd ed. Dhaka: Associates Printing Press. 2011.
27Mowry S, Ogren PJ. Kinetics of methylene blue reduction by ascorbic acid[J].J Chem Educ, 1999, 76(7): 970–974.  
28Masaki H, Atsumi T, Sakurai H. Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation[J].Biochem Biophys Res Commun, 1995, 206(2): 474–479.  
29Huang RP, Wu JX, Fan Y, Adamson ED. UV activates growth factor receptors via reactive oxygen intermediates[J].J Cell Biol, 1996, 133(1): 211–220.  
30Yaar M, Gilchrest BA. Photoageing: mechanism, prevention and therapy[J].Br J Dermatol, 2007, 157(5): 874–887.  
31Tanaka K, Hasegawa J, Asamitsu K, Okamoto T. Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor κB inhibitor, parthenolide[J].J Pharmacol Exp Ther, 2005, 315(2): 624–630.  
32El-Abaseri TB, Putta S, Hansen LA. Ultraviolet irradiation induces keratinocyte proliferation and epidermal hyperplasia through the activation of the epidermal growth factor receptor[J].Carcinogenesis, 2006, 27(2): 225–231.  
33Jurkiewicz BA, Buettner GR. EPR detection of free radicals in UV-irradiated skin: mouse versus human[J].Photochem Photobiol, 1996, 64(6): 918–922.  
34Fisher GJ, Talwar HS, Lin J, Lin P, McPhillips F, Wang Z, Li X, Wan Y, Kang S, Voorhees JJ. Retinoic acid inhibits induction of c-Jun protein by ultraviolet irradiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo[J].J Clin Invest, 1998, 101(6): 1432–1440.  
35Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. Molecular basis of sun-induced premature skin ageing and retinoid antagonism[J].Nature, 1996, 379(6563): 335–339.  
36Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. Pathophysiology of premature skin aging induced by ultraviolet light[J].N Engl J Med, 1997, 337(20): 1419–1428.  
37Shukla V, Vashistha M, Singh SN. Evaluation of antioxidant profile and activity of amalaki (Emblica officinalis), spirulina and wheat grass[J].Indian J Clin Biochem, 2009, 24(1): 70–75.  
 Home | Current Issue | Past Issues | SearchRSS
Copyright © 2013-2018 by JIM Editorial Office. All rights reserved. ISSN 2095-4964; CN 31-2083/R. 沪ICP备110264号