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Journal of Integrative Medicine: Volume 15, 2017   Issue 6,  Pages: 483–494

DOI: 10.1016/S2095-4964(17)60360-1
Research Article
Marker-based standardization and investigation of nutraceutical potential of Indian propolis
1. Nanaware Sadhana (Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411038, Maharashtra, India )
2. Sathiyanarayanan Lohidasan (Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411038, Maharashtra, India )
3. Kakasaheb Ramoo Mahadik (Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411038, Maharashtra, India )
ABSTRACT OBJECTIVE: Propolis, a resinous material collected by honey bees from various plants, has been explored globally for its medicinal and nutritional properties. However, research over Indian propolis is at infancy. This study was designed to investigate nutraceutical potential of Indian propolis. METHODS: In the present study, propolis extract was standardized with respect to markers caffeic acid phenethyl ester, caffeic acid, galangin, luteolin, curcumin, apigenin, pinocembrin and quercetin by new high-performance thin-layer chromatographic (HPTLC) methods. The physico-chemical analysis, residues analysis and in vitro antioxidant activity analysis were performed. Nutraceutical value was examined in terms of fats, fibers, minerals, proteins, polysaccharides, total carbohydrates, and energy value. RESULTS: The developed HPTLC methods were found to be simple, reliable accurate, and the validation parameters were within the limits of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines. Macerated ethanolic extract of propolis (MEEP) was found to have polyphenolic content of (20.99 ± 0.24) mg/g and flavonoids content of (8.39 ± 0.04) mg/g. MEEP was found to comprise of (283.33 ± 51.31) g/kg fats, (30.07 ± 7.30) g/kg fibers, (102.56 ± 2.84) g/kg proteins and (389.36 ± 57.50) g/kg carbohydrate with a calorie value of (38 409.33 ± 6 169.80) kJ/kg. It was found that Indian propolis exhibited high nutraceutical value and showed absence of pesticides and heavy metals. The MEEP showed in vitro antioxidant activity with inhibitory concentration of (12.24 ± 4.64) μg/mL. CONCLUSION: The present work explores Indian propolis as a potential nutritious candidate. The proposed analytical methods can be applied in future screening of the quality of Indian propolis.
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Sadhana N, Lohidasan S, Mahadik KR. Marker-based standardization and investigation of nutraceutical potential of Indian propolis. J Integr Med. 2017; 15(6): 483–494.
1Choudhari MK, Haghniaz R, Rajwade JM, Paknikar KM. Anticancer activity of Indian stingless bee propolis: an in vitro study[J].Evid Based Complement Alternat Med, 2013, 2013: 928280.  
2Santos FA, Bastos EM, Uzeda M, Carvalho MA, Farias LM, Moreira ES, Braga FC. Antibacterial activity of Brazilian propolis and actions against oral anaerobic bacteria[J].J Ethanopharmacol, 2002, 80(1): 1–7.  
3Rathod S, Brahmankar R, Kolte A. Propolis—a natural remedy. Indian J Dent Res Rev. 2012: 50–52.
4Park YK, Alencar SM, Aguiar CL. Botanical origin and chemical composition of Brazilian propolis[J].J Agric Food Chem, 2002, 50(9): 2502–2506.  
5Arul SK, Prabhu T. Extraction of propolis from beehives and characterization of its constituents and medicinal properties: a review. Int J Adv Eng Tech. 2010; I(III): 50–53.
6Purohit A, Joshi K, Kotru B, Kotru S, Ram H. Histological study of antiatherosclerotic effect of propolis in induced hypercholestrolemic male albino rabbits[J].Indian J Fund Appl Life Sci, 2012, 2(2): 384–390.  
7Sawicka D, Car H, Borawska MH, Nikliński J. The anticancer activity of propolis[J].Folia Histochem Cytobiol, 2012, 50(1): 25–37.  
8Yang L, Yan QH, Ma JY, Wang Q, Zhang JW, Xi GX. High performance liquid chromatographic determination of phenolic compounds in propolis[J].Trop J Pharm Res, 2013, 12(5): 771–776.  
9Kimoto T, Arai S, Kohguchi M, Aga M, Nomura Y, Micallef MJ, Kurimoto M, Mito K. Apoptosis and suppression of tumor growth by artepillin C extracted from Brazilian propolis[J].Cancer Detect Prev, 1998, 22(6): 506–515.  
10Khalil ML. Biological activity of bee propolis in health and disease[J].Asian Pac J Cancer Prev, 2006, 7(1): 22–31.  
11Syamsodin, Dewi RM, Kusmardi. Immunomodulatory and in vivo antiplasmodial activities of propolis extracts[J].Am J Pharmacol Toxicol, 2009, 4(3): 75–79.  
12Huang MT, Ma W, Yen P, Xie JG, Han J, Frenkel K, Grunberger D, Conney AH. Inhibitory effects of caffeic acid phenethyl ester (CAPE) on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in mouse skin and the synthesis of DNA, RNA and protein in HeLa cells[J].Carcinogenesis, 1996, 17(4): 761–765.  
13Natarajan K, Singh S, Burke TR Jr, Grunbergert D, Aggarwal BB. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-κB[J].Proc Natl Acad Sci U S A, 1996, 93(17): 9090–9095.  
14Lee YJ, Liao PH, Chen WK, Yang CY. Preferential cytotoxicity of caffeic acid phenethyl ester analogues on oral cancer cells[J].Cancer Lett, 2000, 153(1–2): 51–56.  
15Chaurasiya ND, Ibrahim MA, Muhammad I, Walker LA, Tekwani BL. Monoamine oxidase inhibitory constituents of propolis: kinetics and mechanism of inhibition of recombinant human MAO-A and MAO-B[J].Molecules, 2014, 19(11): 18936–18952.  
16Toit KD, Buthelezi S, Bodenstein J. Anti-inflammatory and antibacterial profiles of selected compounds found in South African propolis[J].S Afr J Sci, 2009, 105: 470–472.  
17Moise A, Liviu AM, Dezmirean D, Bobis O. Nutraceutical properties of Romanian heather honey[J].Nutr Food Sci, 2013, 43(3): 218–227.  
18Marco GD, Canuti L, Impei S, Leonardi D, Canini A. Nutraceutical properties of honey and pollen produced in a natural park[J].Agric Sci, 2012, 3(2): 187–200.  
19Bogdanov S. Honey as nutrient and functional food: a review. Bee Product Sci. 2016: 1–48.
20Bogdanov S, Jurendic T, Sieber R, Gallmann P. Honey for nutrition and health: a review[J].J Am Coll Nutr, 2008, 27(6): 677–689.  
21Ketkar SS, Rathore AS, Lohidasan S, Rao L, Paradkar AR, Mahadik KR. Investigation of the nutraceutical potential of monofloral Indian mustard bee pollen[J]. J Integr Med, 2014, 12(4): 379–389.  
22Danert FC, Zampini C, Ordo?ez R, Maldonado L, Bedascarrasbure E, Isla MI. Nutritional and functional properties of aqueous and hydroalcoholic extracts from Argentinean propolis[J].Nat Prod Commun, 2014, 9(2): 167–170.  
23González-Martín MI, Escuredo O, Revilla I, Vivar-Quintana AM, Coello MC, Riocerezo CP, Moncada GW. Determination of the mineral composition and toxic element contents of propolis by near infrared spectroscopy[J].Sensors (Basel), 2015, 15(11): 27854–27868.  
24Thirugnanasampandan R, Raveendran SB, Jayakumar R. Analysis of chemical composition and bioactive property evaluation of Indian propolis[J].Asian Pac J Trop Biomed, 2012, 2(8): 651–654.  
25Naik DG, Mujumdar AM, Vaidya HS. Anti-inflammatory activity of propolis from Maharashtra, India[J].J Apicult Res, 2013, 52(2): 35–43.  
26Ambardekar R, Gilda S, Mahadik K, Harsulkar A, Paradkar A. Free radical scavenging and anti-inflammatory activity of Indian propolis[J].Pharmacologyonline, 2009, 3: 991–1002.  
27Naik DG, Vaidya HS. Antioxidant properties of volatile oil of Indian propolis[J].J Apiprod Apimed Sci, 2011, 3(2): 89–93.  
28Ramadan A, Soliman G, Mahmoud SS, Nofal SM, Abdel-Rahman RF. Evaluation of the safety and antioxidant activities of Crocus sativus and propolis ethanolic extracts[J].J Saudi Chem Soc, 2012, 16(1): 13–21.  
29Choudhari MK, Punekar SA, Ranade RV, Paknikar KM. Antimicrobial activity of stingless bee (Trigona sp.) propolis used in the folk medicine of Western Maharashtra, India[J].J Ethnopharmacol, 2012, 141(1): 363–367.  
30Wagh VD, Borkar RD. Indian propolis: a potential natural antimicrobial and antifungal agent[J].Int J Pharm Pharm Sci, 2012, 4(4): 12–17.  
31Kalia P, Kumar NR, Harjai K. Phytochemical screening and antibacterial activity of different extracts of propolis[J].Int J Pharm Biol Res, 2013, 3(6): 219–222.  
32Purohit A, Joshi K, Kotru B, Kotru S. Effect of Indian propolis on haematological parameters in experimentally induced hyperlipidemic male albino rabbits[J].Asian J Pharm Clin Res, 2013, 6(1): 17–19.  
33Parolia A, Thomas MS, Kundabala M, Mohan M. Propolis and its potential uses in oral health[J].Int J Med Med Sci, 2010, 2(7): 210–215.  
34Kumar N, Mueen Ahmed KK, Dang R, Shivananda TN, Das K. GC-MS analysis of propolis of Indian origin[J].J Young Pharm, 2009, 1(1): 46–48.  
35Ramnath S, Venkataramegowda S, Singh C. Chemical composition of bee propolis collected from different regions in India by GCMS analysis[J].Int J Pharmacogn Phytochem, 2015, 30(1): 1319–1328.  
36Trusheva B, Trunkova D, Bankova V. Different extraction methods of biologically active components from propolis: a preliminary study[J].Chem Cent J, 2007, 7: 13.  
37Paviani LC, Fiorito G, Sacoda P, Cabral FA. Different solvents for extraction of Brazilian green propolis: composition and extraction yield of phenolic compounds. [2017-03]. http://www.nupeg.ufrn.br/prosciba/prosciba2013/Papers/T2-11.pdf.
38International Conference on Harmonisation Eexpert Working Group. Validation of analytical procedures: text and methodology. Q2 (R1). [2017-03]. https://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf.
39Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent[J].Methods Enzymol, 1999, 299: 152–178.  
40Kumazawa S, Taniguchi M, Suzuki Y, Shimura M, Kwon MS, Nakayama T. Antioxidant activity of polyphenols in carob pods[J].J Agric Food Chem, 2002, 50(2): 373–377.  
41Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables[J].J Uni Chem Technol Metall, 2005, 40(3): 255–260.  
42Jia ZS, Tang MC, Wu JM. The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chem, 1999, 64(4): 555–559.  
43Hindi NKK, Al-Charrakh AH, Naher HS, Abbas AS. Study of chemical analysis of Iraqi propolis and active component of propolis[J].J Sci, 2015, 5(11): 1095–1103.  
44Horwitz W. Official methods of analysis of the Association of Official Analytical Chemists. 15th ed. Washington DC: Association of Official Analytical Chemists Publisher. 1990.
45Satyanarayanan M, Balaram V, Rao TG, Dasaram B, Ramesh SL, Mathur R, Drolia RK. Determination of trace metals in seawater by ICP-MS after preconcentration and matrix separation by dithiocarbamate complexes[J].Indian J Mar Sci, 2007, 36(1): 71–75.  
46Horwitz W, Latimer GW. Soxhlet extraction method. Nebraska: AOAC International. 2005.
47Horwitz W. Official methods of analysis. 12th ed. Washington DC: Association of Official Analytical Chemists Publisher. 1975.
48Bogard JR, Thilsted SH, Marks GC, Wahab MA, Hossain MAR, Jakobsen J, Stangoulis J. Nutrient composition of important fish species in Bangladesh and potential contribution to recommended nutrient intakes[J].J Food Comp Anal, 2015, 42: 120–133.  
49Lowery OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent[J].J Biol Chem, 1951, 193(1): 265–275.  
50Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances[J].Anal Chem, 1956, 28(3): 350–356.  
51Schakel SF, Buzzard IM, Gebhardt SE. Procedures for estimating nutrient values for food composition databases[J].J Food Comp Anal, 1997, 10(2): 102–114.  
52Merrill AL, Watt BK. Energy value of foods—basis and derivation. Washington DC: ARS United States Department of Agriculture. 1973.
53Sathiyanarayanan L, Paradkar AR, Mahadik KR. In vivo and in vitro antioxidant activity of lipid based extract of Bacopa monniera Linn. compared to conventional extract and traditional preparation[J].Eur J Integr Med, 2010, 2(2): 93–101.  
54Rajasekaran A, Sivagnanam G, Xavier R. Nutraceuticals as therapeutic agents: a review[J].Res J Pharm Tech, 2008, 1(4): 328–340.  
55Zhu YZ, Huang SH, Tan BK, Sun J, Whiteman M, Zhu YC. Antioxidants in Chinese herbal medicines: a biochemical perspective[J].Nat Prod Rep, 2004, 21(4): 478–489.  
56Wang X, Sankarapandian K, Cheng Y, Woo SO, Kwon HW, Perumalsamy H, Ahn YJ. Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea[J].BMC Complement Alternat Med, 2016, 16: 65.  
57Marcucci MC. Propolis: chemical composition, biological properties and therapeutic activity[J].Apidologie, 1995, 26(2): 83–99.  
58Directorate–General Health and Consumer Protection. Assessment of the dietary exposure to arsenic, cadmium, lead and mercury of the population of the EU Member States. (2004). [2017-03]. https://ec.europa.eu/food/sites/food/files/safety/docs/cs_contaminants_catalogue_scoop_3-2-11_heavy_metals_report_en.pdf.
59European Food Safety Authority. Scientific opinion[J].EFSA J, 2010, 8(4): 1–146.  
60World Health Organization, Food and Agriculture Organization of the United Nations. Vitamin and mineral requirements in human nutrition. 2nd ed. (2004) [2017-03]. http://apps.who.int/iris/bitstream/10665/42716/1/9241546123.pdf.
61World Health Organization, Food and Agriculture Organization of the United Nations. Fats and fatty acid in human nutrition. (2008) [2017-03]. http://foris.fao.org/preview/25553-0ece4cb94ac52f9a25af77ca5cfba7a8c.pdf.
62World Health Organization, Food and Agriculture Organization of the United Nations, United Nations University. Energy and protein requirements. Geneva: World Health Organisation.1985.
63World Health Organization, Food and Agriculture Organization of the United Nations. Carbohydrates in human nutrition. (1998) [2017-03]. http://www.fao.org/docrep/W8079E/W8079E00.htm.
64World Health Organization, Food and Agriculture Organization of the United Nations, United Nations University. Human energy requirements. (2001) [2017-03]. http://www.fao.org/3/a-y5686e.pdf.
65Banskota AH, Tezuka Y, Adnyana IK, Midorikawa K, Matsushige K, Message D, Huertas AA, Kadota S. Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China[J].J Ethnopharmacol, 2000, 72(1–2): 239–246.  
66Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina[J].J Ethnopharmacol, 2000, 71(1–2): 109–114.  
67Gardana C, Scaglianti M, Pietta P, Simonetti P. Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography-tandem mass spectrometry[J].J Pharma Biomed Anal, 2007, 45(3): 390–399.  
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