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Journal of Chinese Integrative Medicine: Volume 10, 2012   Issue 10,  Pages: 1162-1170

DOI: 10.3736/jcim20121014
Original Experimental Research
Synergistic effects of ethyl acetate fraction of Ficus septica Burm. f. and doxorubicin chemotherapy on T47D human breast cancer cell line
1. Agung E. Nugroho (Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta 55281, Indonesia E-mail: E-mail: agungendronugroho@yahoo.com)
2. Adam Hermawan (Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta 55281, Indonesia )
3. Dyaningtyas D. P. Putri (Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta 55281, Indonesia )
4. Edy Meiyanto (Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta 55281, Indonesia )
5. Lukman Hakim (Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta 55281, Indonesia )
OBJECTIVE: Previously, ethanolic extract of Ficus septica Burm. f. (Moraceae) leaves and its ethyl acetate soluble fraction (EASF) exhibited potent cytotoxic effects on T47D breast cancer cells. In the present study, we further investigated the effects of EASF of ethanolic extract of F. septica leaves in combination with doxorubicin on T47D breast cancer cell line in cytotoxicity, cell cycle arrest and apoptosis induction.
METHODS: The cytotoxic effect analysis on T47D cells was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Analysis of cell cycle distribution was performed using a flowcytometer and the data were analyzed using ModFit LT 3.0 program. Apoptosis assay was carried out by double staining method using ethydium bromide-acridin orange. The expression of cleaved-poly ADP-ribose polymerase in the T47D cell line was identified using immunohistochemical techniques.
RESULTS: The combination of doxorubicin (2 to 8 nmol/L) with EASF (0.875 to 7 μg/mL) more potently inhibited cell growth than the single treatment of doxorubicin in T47D cells. In addition, the combination of doxorubicin and EASF could increase the incidence of cells undergoing apoptosis. EASF was found to improve cytotoxic effect of doxorubicin by changing the inhibition of cell cycle G2/M to G1 phase. The combination also exhibited a more intensive stimulatory effect on cleaved-PARP expression in T47D cells than the single treatment.
CONCLUSION: It is concluded that EASF may enhance doxorubicin activities in T47D cells by inducing apoptosis and cell cycle arrest.
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Please cite this article as:
Nugroho AE, Hermawan A, Putri DDP, Meiyanto E, Hakim L. Synergistic effects of ethyl acetate fraction of Ficus septica Burm. f. and doxorubicin chemotherapy on T47D human breast cancer cell line. J Chin Integr Med / Zhong Xi Yi Jie He Xue Bao. 2012; 10(10): 1162-1170.
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