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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 94-96.doi: 10.3736/jcim20040205

• Original Clinical Research • Previous Articles     Next Articles

Effect of Xiangdan Injection on mRNA expression of endothelial vaso-active factors of patients with coronary heart disease and blood stasis

Shi-da Wu1, Jing Wang1, Shou-chun Chen2, Jun-bo Xu1, Qiang Zheng1, Ya-fei Yan1, Tian-ming Wen1, Yan-rong Tang1   

  1. 1. Department of Cardiology, Chengdu First People,s Hospital,Chengdu,Sichuan Province 610016,China
    2. Chengdu Di’ao Pharmaceutical (Group) Co. Ltd., Chengdu,Sichuan Province 610041,China;
  • Online:2004-03-20 Published:2018-10-25

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Objective: To evaluate the effect of Xiangdan Injection on mRNA expression of the endothelial vaso-active factors of patients with coronary heart disease and blood stasis.Methods: Fifty-six patients were randomly divided into two groups: twenty-eight patients were treated according to the therapeutic guide for coronary heart disease as the control group and 28 were given the same treatment plus Xiangdan Injection as the treated group. The expressions of ET-1 and eNOS mRNA were examined with RT-PCR before experiment and ten days later. Results: The positive rate of eNOS mRNA of the treated group increased, while the positive rate of ET-1 mRNA of the treated group decreased after ten day's treatment, with significant differences as compared with that before the experiment. Xiangdan Injection up-regulated the eNOS mRNA expression and suppressed the ET-1 mRNA expression. Changes of expression were not observed in the control group. Conclusion: Xiangdan Injection improves the endothelial function of patients with coronary heart disease and blood stasis by regulating the expressions of ET-1 and eNOS mRNA.

Key words: Xiangdan Injection, vascular endothelial function, endothelial nitric-oxide synthase, endothelin-1, mRNA

CLC Number: 

  • R541.4

Figure 1

Agarose gel electrophoresis of total RNAM: Marker; 1: Total RNA of whole blood sample 1; 2: Total RNA of whole blood sample 2 ET-1: P1 5′-GATTATTTGCTCATGATTT-3′ P2 5′-TCACCAATGTGCTCGGTTG-3′ eNOS: P1 5′-TAGCCAAAGTCACCATCGT-3′ P2 5′-GAGCCATACAGGATTGTCG-3′"

Tab 1

Positive cases of ET-1 and eNOS mRNA expression in 2 groups"

Group n ET-1 mRNA
positive cases		 eNOS mRNA
positive cases
Control group
Before treatment 28 24 15
After treatment 28 23 15
Treated group
Before treatment 28 23 14
After treatment 28 16*△ 22*△

Figure 2

Agarose gel electrophoresis of ET-1 geneM: Marker; 1: Positive control, 600 bp; 2: Negative sample; 3: Positive sample, 500 bp"

Figure 3

Agarose gel electrophoresis of eNOS geneM: Marker; 1: Positive control, 600 bp; 2: Negative sample; 3: Positive sample, 400 bp"

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