Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 94-96.doi: 10.3736/jcim20040205

• Original Clinical Research • Previous Articles     Next Articles

Effect of Xiangdan Injection on mRNA expression of endothelial vaso-active factors of patients with coronary heart disease and blood stasis

Shi-da Wu1, Jing Wang1, Shou-chun Chen2, Jun-bo Xu1, Qiang Zheng1, Ya-fei Yan1, Tian-ming Wen1, Yan-rong Tang1   

  1. 1. Department of Cardiology, Chengdu First People,s Hospital,Chengdu,Sichuan Province 610016,China
    2. Chengdu Di’ao Pharmaceutical (Group) Co. Ltd., Chengdu,Sichuan Province 610041,China;
  • Online:2004-03-20 Published:2018-10-25

Objective: To evaluate the effect of Xiangdan Injection on mRNA expression of the endothelial vaso-active factors of patients with coronary heart disease and blood stasis.Methods: Fifty-six patients were randomly divided into two groups: twenty-eight patients were treated according to the therapeutic guide for coronary heart disease as the control group and 28 were given the same treatment plus Xiangdan Injection as the treated group. The expressions of ET-1 and eNOS mRNA were examined with RT-PCR before experiment and ten days later. Results: The positive rate of eNOS mRNA of the treated group increased, while the positive rate of ET-1 mRNA of the treated group decreased after ten day's treatment, with significant differences as compared with that before the experiment. Xiangdan Injection up-regulated the eNOS mRNA expression and suppressed the ET-1 mRNA expression. Changes of expression were not observed in the control group. Conclusion: Xiangdan Injection improves the endothelial function of patients with coronary heart disease and blood stasis by regulating the expressions of ET-1 and eNOS mRNA.

Key words: Xiangdan Injection, vascular endothelial function, endothelial nitric-oxide synthase, endothelin-1, mRNA

CLC Number: 

  • R541.4

Figure 1

Agarose gel electrophoresis of total RNAM: Marker; 1: Total RNA of whole blood sample 1; 2: Total RNA of whole blood sample 2 ET-1: P1 5′-GATTATTTGCTCATGATTT-3′ P2 5′-TCACCAATGTGCTCGGTTG-3′ eNOS: P1 5′-TAGCCAAAGTCACCATCGT-3′ P2 5′-GAGCCATACAGGATTGTCG-3′"

Tab 1

Positive cases of ET-1 and eNOS mRNA expression in 2 groups"

Group n ET-1 mRNA
positive cases
positive cases
Control group
Before treatment 28 24 15
After treatment 28 23 15
Treated group
Before treatment 28 23 14
After treatment 28 16* 22*

Figure 2

Agarose gel electrophoresis of ET-1 geneM: Marker; 1: Positive control, 600 bp; 2: Negative sample; 3: Positive sample, 500 bp"

Figure 3

Agarose gel electrophoresis of eNOS geneM: Marker; 1: Positive control, 600 bp; 2: Negative sample; 3: Positive sample, 400 bp"

[1] 王奇, 陈云波, 赖世隆 , 等. 血府逐瘀汤对血瘀证兔模型血清损伤的血管内皮细胞内分泌功能的影响[J]. 中国实验方剂学杂志, 2002,8(2):12-14
[2] 陈可冀 . 关于瘀血证的国内外进展[J]. 临床荟萃, 1987,2(2):16-18
[3] Fukuchi M, Giaid A . Endothelial expression of endothelial nitric oxide synthase and endothelin-1 in human coronary artery disease.Specific reference to underlying lesion[J]. Lab Invest, 1999,79(6):659-670
[4] 陈颂, 葛金文 . 血瘀证实质与血管内皮细胞关系的研究概况[J]. 湖南中医学院学报, 2001,21(1):67-69
doi: 10.3969/j.issn.1674-070X.2001.01.036
[5] 巫刚, 邹才华, 毛兵 , 等. 急性胰腺炎患者血浆内皮素和一氧化氮变化与血瘀证关系的探讨[J]. 中国中西医结合杂志, 1999,19(12):715-717
[6] 王春喜, 齐清会, 吴咸中 , 等. 内皮素、一氧化氮等内皮细胞活性因子与动脉硬化闭塞症血瘀证关系的研究[J]. 中国中西医结合杂志, 1999,19(8):463-465
[7] 王阶, 李建生, 姚魁武 , 等. 血瘀证量化诊断及病证结合研究[J]. 中西医结合学报, 2003,1(1):21-24
Wang J, Li JS, Yao KW , et al. Research on quantified diagnosis and combining diseases with syndrome of blood stasis[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(1):21-24
[1] Xiao-lei Hou, Bai-ling Li, Lei Zhao, Sheng-dong Huang, Zhi-yun Xu, Guan-xin Zhang. Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion. Journal of Chinese Integrative Medicine, 2008, 6(4): 381-386.
[2] Yan-sheng Huang, Shu-ren Wang, Yan-fang Zhi, Shen-yan Kong, Lin Sun​, Yu Wu​, Jian-min Lu​, Fu-min Dai​. Effects of Xuezhikang Capsules on vascular endothelial function and redox status in patients with coronary heart disease. Journal of Chinese Integrative Medicine, 2006, 4(3): 251-255.
[3] Jian-xin Wu, Rong-xiang Niu, Cong-li Dong. Effects of Xinmailong, Xiangdan and Shenmai Injection on early kidney damage induced by toxin of grass carp bile. Journal of Chinese Integrative Medicine, 2004, 2(1): 33-35.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228