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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 119-127.doi: 10.3736/jcim20050211

• Original Experimental Research • Previous Articles     Next Articles

Expression of NF-κB and COX-2 mRNA in rats with phlegm obstruction due to lung-deficiency

Ping Wang1,Xiu-yan Wu2,Mao-lin Zhang1,Dai-zhi Tian3,Song-lin Liu4   

  1. 1. Department of Basic Theory of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430061, China
    2. Department of Traditional Chinese Internal Medicine, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430061, China
    3. Central Laboratory, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430061, China
  • Received:2004-05-18 Online:2005-03-20 Published:2005-03-20
  • Contact: Ping Wang E-mail:pwang54@yahoo.com.cn

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Objective

To study the role of nuclear factor-kappa B (NF-κB) and cycloxygenase-2 (COX-2) in the onset of phlegm obstruction due to lung-deficiency in rats and the therapeutic mechanism of Huatan Recipe.

Methods

Twenty-four SD rats were randomly divided into normal group, model group and treatment group, with 8 rats in each group. The rats in the model group and treatment group were exposed to sulfur dioxide and cold wind to establish the rat model of phlegm obstruction due to lung-deficiency, and the rats in the treatment group were also treated with Huatan Recipe, a compound traditional Chinese medicine. The expression of NF-κB in the bronchial epithelial cells of the rats was tested with the method of immunohistochemistry, and the COX-2 mRNA in the lung tissues of the rats was measured by using reverse transcription-polymerase chain reaction.

Results

The expressions of NF-κB and COX-2 mRNA in rats of the model group were higher than those of the normal group (P<0.01), and the expressions of NF-κB and COX-2 mRNA in rats of the treatment group were obviously lower than those of the model group (P<0.01).

Conclusion

The NF-κB and COX-2 play an important role in the onset of phlegm obstruction in rats. Huatan Recipe may prevent the development of phlegm obstruction by down-regulating the expressions of NF-κB and COX-2 mRNA.

Key words: Phlegm obstruction due to lung-deficiency, Nuclear factor-kappa B, Cycloxygenase-2

CLC Number: 

  • R256.1

Tab 1

NF-κB level in bronchial epithelial cells ($\bar{x}$±s)"

Group n NF-κB （%）
Normal 8 22±5**
Model 8 53±4
Treatment 8 27±5**

Tab 2

Relative expression of COX-2 mRNA in lung tissue ($\bar{x}$±s)"

Group n COX-2 mRNA
Normal 8 0.91±0.11**
Model 8 2.22±0.28
Treatment 8 1.22±0.16**

Fig 1

RT-PCR of COX-2 mRNA in lung tissue"

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