牛珀至宝微丸对内毒素休克大鼠脑神经型一氧化氮合酶表达的影响

doi:10.3736/jcim20050210

Abstract

Objective

To investigate the effect of Niupo Zhibao Pellet (NPZBP) on the expression of neuronal nitric oxide synthase (nNOS) in the brain of endotoxin-induced shock rats.

Methods

SD rats were randomly divided into normal control group, endotoxin-induced shock model group and NPZBP-treated group. Lipopolysaccharide (LPS) (1.5 mg/kg i.v.) and D-galactosamine (D-GalN) (100 mg/kg i.p.) were administered to the rats in endotoxin-induced shock model group, as well as to the rats in NPZBP-treated group after seven-day treatment, to induce the shock. The expression of nNOS in the brain of the rats in each of the 3 groups was measured by immunohistochemical methods.

Results

In the 3 groups, nNOS immuno-positive cells distributed widely in layer Ⅱ, Ⅲ, Ⅳ of the cerebral cortex, the molecular layer of hippocampus, the polymorphic layer of the dentate gyrus, the reticular formation of brain stem, and the molecular, granular and Purkinje cell layer of the cerebellar cortex. The number of immuno-positive cells in the NPZBP-treated group was slightly higher than that of the normal control group, and signifficantly lower than that of the model group (P<0.05) in many regions of the brain, including cerebral cortex, hippocampus, brain stem and cerebellar cortex.

Conclusion

NPZBP can inhibit the over-expression of nNOS in wide area of the brain in endotoxin-induced shock rats.

Key words: Niupo Zhibao Pellet, Endotoxin-induced shock, Neuronal nitric oxide synthase, Immuno-histochemistry, Animals, Laboratory

CLC Number:

R285.5

Jian-hong Zhou, Dong-feng Chen, Shao-hui Du, Hui Li, Yi-wei Li, Ru-dong Deng, Sai-xia Zhang. Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 115-118.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050210

http://www.jcimjournal.com/EN/Y2005/V3/I2/115

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References 12

[1]	Glauser MP, Zanetti G, Baumgarther JD , et al. Septic shock: pathogenesis[J]. Lancet, 1991,338(8769):732-736 doi: 10.1016/0140-6736(91)91452-Z
[2]	万兰清, 马超英, 耿耘 , 等. 开闭固脱法治疗感染性休克106例的临床与实验研究[J]. 中国病理生理杂志, 1994,10(增刊):60-63
[3]	Anggard E . Nitric oxide: mediator, murder, and medicine[J]. Lancet, 1994,343(8907):1199-1206 doi: 10.1016/S0140-6736(94)92405-8 pmid: 7909873
[4]	黎晖, 杜少辉, 李伊为 , 等. 牛珀至宝微丸对内毒素休克鼠肺组织中一氧化氮合酶活性的影响[J]. 广州中医药大学学报, 2002,19(4):305-308
[5]	杜少辉, 周大桥, 张悦 , 等. 牛珀至宝微丸对内毒素休克时蛋白激酶C调控一氧化氮生成的影响[J]. 中国中西医结合急救杂志, 2002,9(4):189-193 doi: 10.3321/j.issn:1008-9691.2002.04.003
[6]	李伊为, 邓汝东, 陈东风 , 等. 牛珀至宝微丸对内毒素休克肾组织诱生型一氧化氮合酶表达的影响[J]. 解剖学研究, 2003,25(1):13-15 doi: 10.3969/j.issn.1671-0770.2003.01.005
[7]	Wichterman KA, Baue AE, Chaudry IH . Sepsis and septic shock--a review of laboratory models and a proposal[J]. J Surg Res, 1980,29(2):189-201 doi: 10.1016/0022-4804(80)90037-2 pmid: 6997619
[8]	Vincent AM, Maiese K . Nitric oxide induction of neuronal endonuclease activity in programmed cell death[J]. Exp Cell Res, 1999,246(2):290-300 doi: 10.1006/excr.1998.4282 pmid: 9925743
[9]	Brown GC, Cooper CE . Nanomolar concentrations of nitric oxide reversibly inhibit synaptosomal respiration by competing with oxygen at cytochrome oxidase[J]. FEBS Lett, 1994,356(2-3):295-298 doi: 10.1016/0014-5793(94)01290-3
[10]	Samdani AF, Dawson TM, Dawson VL . Nitric oxide synthase in models of focal ischemia[J]. Stroke, 1997,28(6):1283-1288 doi: 10.1161/01.STR.28.6.1283 pmid: 9183363
[11]	Schulz JB, Matthews RT, Jenkins BG , et al. Blockade of neuronal nitric oxide synthase protects against excitotoxicity in vivo[J]. J Neurosci, 1995,15(12):8419-8429 pmid: 8613773
[12]	Hara H, Huang PL, Panhian N , et al. Reduced brain edema and infarction volume in mice lacking the neuronal isoform of nitric oxide synthase after transient MCA occlusion[J]. J Cereb Blood Flow Metab, 1996,16(4):605-611 doi: 10.1097/00004647-199607000-00010

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Group	n	Number of nNOS immuno-positive cells
Group	n	Cerebral cortex	Hippocampus	Brain stem	Cerebellum
Normal control	50	18.2±1.9	9.2±2.6	7.3±1.7	3.3±1.3
Model	50	23.7±3.8*	14.6±2.8*	11.4±1.5*	6.4±1.2*
NPZBP-treated	50	21.4±1.6^△	12.6±2.8^△	9.4±1.5^△	4.1±1.3^△

Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats

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