In vivo antianxiety and antidepressant activity of Murraya paniculata leaf extracts

doi:10.1016/S2095-4964(17)60352-2

Abstract

Objective

Different parts of Murraya paniculata have been used traditionally for treating several ailments including mental disorders. The present study was designed to evaluate the antianxiety and antidepressant potential of M. paniculata leaves using elevated plus maze model and forced swim test, respectively.

Methods

Extracts of M. paniculata made with petroleum ether (60-80 °C), chloroform, ethanol and water were evaluated for antianxiety and antidepressant activity. The anxiolytic chloroform extract was subjected to column chromatography, yielding five fractions (F₁-F₅). Fraction F₅ (100 mg/kg), which showed notable anxiolytic activity, was further chromatographed to get four subfractions (F_5.1-F_5.4). Simultaneously, the ethanol extract was partitioned with ethyl acetate to obtain ethyl acetate soluble fraction (EASF) and ethyl acetate insoluble fraction. Phytochemical screening of bioactive extracts/fractions and detection of mahanimbine in M. paniculata leaf extract by thin-layer chromatography was also carried out.

Results

Fraction F_5.3 (25 mg/kg) and EASF (20 mg/kg) showed significant anxiolytic and antidepressant activity, respectively. Thin-layer chromatography and phytochemical screening demonstrated the absence of mahanimbine in M. paniculata leaves. Coumarins were observed to be responsible for the anxiolytic activity.

Conclusion

The results validate the traditional use of M. paniculata leaves in the treatment of mental disorders.

Key words: Murraya paniculata, Anxiety, Depressant, Coumarins, Mahanimbine, Plants, medicinal

Pragya Sharma, Sonali Batra, Ashwani Kumar, Anupam Sharma. In vivo antianxiety and antidepressant activity of Murraya paniculata leaf extracts. Journal of Integrative Medicine, 2017, 15(4): 320-325.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.1016/S2095-4964(17)60352-2

http://www.jcimjournal.com/EN/Y2017/V15/I4/320

trendmd

References

1 National Institue of Science Communication and Information Resources. The wealth of India. New Delhi: Raw Materials, Publication and Information Directorate, Council of Scientific and Industrial Research. 1962: 446-448.
2 Noolu B, Ajumeera R, Chauhan A, Nagalla B, Manchala R, Ismail A.Murraya koenigii leaf extract inhibits proteasome activity and induces cell death in breast cancer cells. BMC Complement Altern Med. 2013; 13: 7.
3 Kirtikar KR, Basu BD.Indian medicinal plants. Allahabad: Lalit Mohan Basuan Co. 1935: 4.
4 Saqib F, Ahmed MG, Janbaz KH, Dewanjee S, Jaafar HZ, Zia-Ul-Haq M. Validation of ethnopharmacological uses ofMurraya paniculata in disorders of diarrhea, asthma and hypertension. BMC Complement Altern Med. 2015; 15: 319.
5 Dahiya J, Singh J, Kumar A, Sharma A.Isolation, characterization and quantification of an anxiolytic constituent—mahanimbine, fromMurraya koenigii Linn. Spreng leaves. J Ethnopharmacol. 2016; 193: 706-711.
6 Organisation for Economic Co-operation and Development. OECD guidelines for the testing of chemicals: acute oral toxicity—acute toxic class method (423). (2001-12-17) [2016]. https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/ oecd/oecd_gl423.pdf.
7 Pellow S, Chopin P, File SE, Briley M.Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rats.J Neurosci Methods. 1985; 14(3): 149-167.
8 Lister RG.Ethologically based animal models of anxiety disorders.Pharmacol Ther. 1990; 46(3): 321-340.
9 Porsolt RD, Le Pichon M, Jalfre M.Depression: a new animal model sensitive to antidepressant treatments. Nature.1977; 266(5604): 730-732.
10 Porsolt RD, Anton G, Blavet N, Jalfre M.Behavioural despair in rats: a new model sensitive to antidepressant treatments.Eur J Pharmacol. 1978; 47(4): 379-391.
11 Farnsworth NR.Biological and phytochemical screening of plants.J Pharm Sci. 1966; 55(3): 225-276.
12 DiMatteo MR, Lepper HS, Croghan TW. Depression is a risk factor for noncompliance with medical treatment: metaanalysis of the effects of anxiety and depression on patient adherence.Arch Intern Med. 2000; 160(14): 2101-2107.
13 Sherbourne CD, Wells KB, Meredith LS, Jackson CA, Camp P.Comorbid anxiety disorder and the functioning and well-being of chronically ill patients of general medical providers.Arch Gen Psychiatry. 1996; 53(10): 889-895.
14 Baum C, Kennedy DL, Forbes MB, Jones JK.Drug use in the United States in 1981.JAMA. 1984; 251(10): 1293-1297.
15 Swartz M, Landerman R, George LK, Melville ML, Blazer D, Smith K.Benzodiazepine anti-anxiety agents: prevalence and correlates of use in a southern community.Am J Public Health. 1991; 81(5): 592-596.
16 Kumar S, Malhotra R, Kumar D.Euphorbia hirta: its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacogn Rev. 2010; 4(7): 58-61.
17 Anuradha H, Srikumar BN, Shankaranarayana Rao BS, Lakshmana M.Euphorbia hirta reverses chronic stressinduced anxiety and mediates its action through the GABAA receptor benzodiazepine receptor-Cl(-) channel complex. J Neural Transm (Vienna). 2008; 115(1): 35-42.
18 Rahman AU, Shabbir M, Sultani SZ, Jabbar A, Choudhary MI.Cinnamates and coumarins from the leaves ofMurraya paniculata. Phytochemistry. 1997; 44: 683-685.
19 Kumar D, Bhat ZA, Kumar V, Shah MY.Coumarins fromAngelica archangelica Linn. and their effects on anxietylike behavior. Prog Neuropsychopharmacol Biol Psychiatry. 2008; 40: 180-186.
20 Monsef-Esfahani HR, Amini M, Goodarzi N, Saiedmohammadi F, Hajiaghaee R, Faramarzi MA, Tofighi Z, Ghahremani MH.Coumarin compounds ofBiebersteinia multifida roots show potential anxiolytic effects in mice. Daru. 2013; 21(1): 51.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Fu-rong Du , Bin Zhang. Study on English translation for rhetoric devices in Neijing; intertextuality and synecdoche. Journal of Chinese Integrative Medicine, 2010, 8(12): 1207-1210
[2]	Mei-jun Lü, Hui-yong Zhang, Zhe Zhang, Guan-lin Yang. Measurement of health-related quality of life in coronary heart disease: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1277-1285
[3]	Hua-fa Que, Han-jun Tang, Lin-yang Wang, Hong-yu Dai, Shi-yun Zhang, Hai-guang Qin, Xiao-dong Liu, Huan-yu Xiang, Zhen Zhang. Study on wound healing mechanism of replenishing qi and dissipating stagnation therapy for chronic skin ulcers. Journal of Chinese Integrative Medicine, 2005, 3(3): 243-246
[4]	Wen-hui Ma, Xiao-hong Sun. Discussion of time and space differentiation of three-yin and three-yang in Shanghan Lun. Journal of Chinese Integrative Medicine, 2005, 3(4): 257-259
[5]	Dong Yang, Shi Jian-rong. Biological research evaluating the Chinese medical theory of the association of the kidney with the ears. Journal of Chinese Integrative Medicine, 2012, 10(2): 128-134
[6]	Xiao-hui Sun, Sheng Liu . External Chinese medical therapy for pain associated with hyperplastic disease of the breast: Study protocol of a randomized, double-blind, multicenter, controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(4): 384-389
[7]	Hai-bo Yu, Cheng-en Pan, Wu-jun Wu, Si-hai Zhao, Hui-feng Zhang. Effects of resveratrol on matrix metalloproteinase-9 expression in hepatoma cells. Journal of Chinese Integrative Medicine, 2008, 6(3): 270-273
[8]	Xiao-long Wei, Rui Feng, Zhi-qing Zhao, Zai-ping Jing. Efficacy of Chinese herbal medicine Mailuo Shutong Granule and Hirudoid cream for chronic venous disorder-induced pigmentation in lower extremities: A prospective, randomized controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(8): 866-870
[9]	Hui Han , Li-min Wu , Ming-xiang Han, Wen-ming Yang , Dao-jun Xie , Zhao-hui Fang , Dan-chen Chu , Fang Fang . Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(10): 1140-1148
[10]	ZG Li. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: An analysis of the Causes of Diseases (Part 2). Journal of Chinese Integrative Medicine, 2009, 7(4): 383-388