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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 270-273.doi: 10.3736/jcim20080310

• Original Experimental Research • Previous Articles     Next Articles

Effects of resveratrol on matrix metalloproteinase-9 expression in hepatoma cells

Hai-bo Yu1(), Cheng-en Pan1, Wu-jun Wu1, Si-hai Zhao2, Hui-feng Zhang3   

  1. 1. Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School of Xi'an Jiao tong University, Xi’an, Shaanxi Province 710061, China;
    2. Department of Immunology and Microbiology, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China
    3. Intensive Care and Treatment Center, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, China
  • Received:2007-05-18 Online:2008-03-20 Published:2008-03-15
  • Contact: HB Yu

Objective: To observe the effects of resveratrol on proliferation of human hepatocellular carcinoma cell line SMMC-7721 cells and expression of matrix metalloproteinase-9 (MMP-9) in vitro.Methods: SMMC-7721 cells were treated with different concentrations of resveratrol for 24, 48 and 72 h, respectively. The effect of resveratrol on proliferation of SMMC-7721 cells was assessed with methyl thiazolyl tetrazolium (MTT). The expression of MMP-9 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). MMP-9 protein was identified by Western blot analysis.Results: Resveratrol could inhibit the proliferation of SMMC-7721 cells with dose- and time-dependent effects. Moreover, both MMP-9 mRNA expression and MMP-9 protein production were markedly reduced after resveratrol treatment.Conclusion: Resveratrol can inhibit the proliferation of SMMC-7721 cells and down-regulate MMP-9 expression. It is presumed that resveratrol may suppress the invasion and metastasis of hepatocellular carcinoma.

Key words: resveratrol, liver cancer, matrix metalloproteinase-9

CLC Number: 

  • R735.7

Table 1

Growth-inhibition of resveratrol on human hepatocellular carcinoma cell line SMMC-7721 ($\bar{x}$±s, %)"

Concentration of Res n Growth-inhibiting rate at different action time
24 h 48 h 72 h
10 μmol/L 4 2.1±1.2 2.9±1.9 4.9±3.7
50 μmol/L 4 10.0±5.7 12.6±3.6 15.7±4.2
100 μmol/L 4 14.0±4.8 18.2±4.3 29.9±14.5
200 μmol/L 4 27.5±3.6 37.7±5.1 45.0±18.1
500 μmol/L 4 57.5±2.3 67.8±2.9 79.0±1.3

Figure 1

Inhibition of Res on expression of MMP-9 mRNA in SMMC-7721 cells A: MMP-9 mRNA expression under different concentrations of Res treatment for 24 h; B: Relative content of MMP-9 mRNA level."

Figure 2

Inhibition of Res on MMP-9 protein expression in SMMC-7721 cells A: MMP-9 protein expression under different concentrations of Res treatment for 24 h; B: Relative content of MMP-9 protein level."

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