In vivo antianxiety and antidepressant activity of Murraya paniculata leaf extracts

doi:10.1016/S2095-4964(17)60352-2

Abstract

Objective

Different parts of Murraya paniculata have been used traditionally for treating several ailments including mental disorders. The present study was designed to evaluate the antianxiety and antidepressant potential of M. paniculata leaves using elevated plus maze model and forced swim test, respectively.

Methods

Extracts of M. paniculata made with petroleum ether (60-80 °C), chloroform, ethanol and water were evaluated for antianxiety and antidepressant activity. The anxiolytic chloroform extract was subjected to column chromatography, yielding five fractions (F₁-F₅). Fraction F₅ (100 mg/kg), which showed notable anxiolytic activity, was further chromatographed to get four subfractions (F_5.1-F_5.4). Simultaneously, the ethanol extract was partitioned with ethyl acetate to obtain ethyl acetate soluble fraction (EASF) and ethyl acetate insoluble fraction. Phytochemical screening of bioactive extracts/fractions and detection of mahanimbine in M. paniculata leaf extract by thin-layer chromatography was also carried out.

Results

Fraction F_5.3 (25 mg/kg) and EASF (20 mg/kg) showed significant anxiolytic and antidepressant activity, respectively. Thin-layer chromatography and phytochemical screening demonstrated the absence of mahanimbine in M. paniculata leaves. Coumarins were observed to be responsible for the anxiolytic activity.

Conclusion

The results validate the traditional use of M. paniculata leaves in the treatment of mental disorders.

Key words: Murraya paniculata, Anxiety, Depressant, Coumarins, Mahanimbine, Plants, medicinal

Pragya Sharma, Sonali Batra, Ashwani Kumar, Anupam Sharma. In vivo antianxiety and antidepressant activity of Murraya paniculata leaf extracts. Journal of Integrative Medicine, 2017, 15(4): 320-325.

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