银杏内酯对犬脑血流量的影响

doi:10.3736/jcim20050115

Abstract

Objective

To observe the effects of ginkgolide (GL) on the cerebral blood flow in dogs.

Methods

Dogs anesthetized with sodium pentobarbital were randomly divided into 5 groups, with 4 dogs in each group. Ginkgolide of 4.86, 14.6 and 43.7 mg/kg and Xingling Granule of 0.22 g/kg were administered by gavage to the dogs in each of 4 groups. The dogs in the other group were administered with edible oil (1 ml/kg) as control group. The cerebral blood flow, systolic blood pressure, diastolic blood pressure, mean arterial blood pressure and electro-cardiogram of the dogs were measured at different times after the administration.

Results

Ginkgolide of 4.86, 14.6 and 43.7 mg/kg had no obvious effects on the blood pressure and the heart rate. Ginkgolide of 14.6 and 43.7 mg/kg increased the cerebral blood flow 90 minutes after administration, and ginkgolide of 43.7 mg/kg obviously decreased the cerebral vascular resistance 150 minutes after administration.

Conclusion

Ginkgolide can increase the cerebral blood flow and decrease the cerebral vascular resistance, and it has no obvious effects on blood pressure and heart rate in dogs.

Key words: Ginkgo biloba, Brain, Regional blood flow

CLC Number:

R285.6

Jiang-ping Xu, Lin Li, Li-sha Sun. Effects of ginkgolide on cerebral blood flow in dogs. Journal of Chinese Integrative Medicine, 2005, 3(1): 50-53.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050115

http://www.jcimjournal.com/EN/Y2005/V3/I1/50

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Figures/Tables 2

Tab 1

Effects of ginkgolide on internal carotid blood flow and cerebral vascular resistance of anesthetized dogs ($\bar{x}$±s)"

Group	n	Internal carotid blood flow （ml/min）	Cerebral vascular resistance (kPaml^-1100 g^-1min^-1)
Control
Before administration	4	122.3±6.1	0.11±0.03
60 min after	4	125.5±2.5	0.12±0.03
90 min after	4	124.5±6.4	0.12±0.03
120 min after	4	126.5±7.0	0.12±0.02
150 min after	4	127.5±4.7	0.11±0.03
180 min after	4	129.0±6.6	0.11±0.02
240 min after	4	125.0±1.2	0.11±0.03
300 min after	4	124.0±5.7	0.11±0.02
GL (4.86 mg/kg)
Before administration	4	124.8±12.6	0.11±0.03
60 min after	4	125.0±12.9	0.11±0.02
90 min after	4	130. 0±8.5	0.11±0.03
120 min after	4	129.0±11.6	0.11±0.03
150 min after	4	133.5±13.9	0.11±0.02
180 min after	4	134.0±16.6	0.11±0.02
240 min after	4	128.0±15.4	0.11±0.03
300 min after	4	127.0±12.3	0.11±0.04
GL (14.6 mg/kg)
Before administration	4	117.0±10.6	0.10±0.02
60 min after	4	134.5±11.7*	0.08±0.02
90 min after	4	141.0±9.9**^Δ	0.07±0.02
120 min after	4	143.8±8.9**^Δ	0.07±0.02
150 min after	4	141.5±9.3*	0.07±0.02
180 min after	4	140.0±9.1*	0.08±0.02
240 min after	4	135.0±6.2*	0.08±0.02
300 min after	4	129.2±8.8	0.08±0.02
GL (43.7 mg/kg)
Before administration	4	124.5±9.3	0.10±0.02
60 min after	4	145.0±15.1**	0.10±0.03
90 min after	4	161.0±10.5**^ΔΔ	0.08±0.02
120 min after	4	163.5±11.4**	0.08±0.02
150 min after	4	171.0±10.6**^ΔΔ	0.07±0.02*
180 min after	4	167.5±15.4**^ΔΔ	0.07±0.02*
240 min after	4	160.5±14.4*^ΔΔ	0.07±0.02*
300 min after	4	154.5±13.0*^Δ	0.07±0.01*
Xingling Granule
Before administration	4	128.5±15.9	0.11±0.02
60 min after	4	138.5±5.0*^Δ	0.07±0.01*
90 min after	4	158.0±17.4*^Δ	0.07±0.01*^Δ
120 min after	4	168.5±17.0*^ΔΔ	0.07±0.01*
150 min after	4	178.0±14.9**^ΔΔ	0.07±0.01**^Δ
180 min after	4	187.0±8.3**^ΔΔ	0.07±0.01*
240 min after	4	185.2±10.4*^ΔΔ	0.08±0.01*
300 min after	4	189.0±16.8**^ΔΔ	0.09±0.01

Tab 1

Tab 2

Effects of ginkgolide on blood pressure and heart rate of anesthetized dogs ($\bar{x}$±s)"

Group	n	Mean arterial blood pressure （kPa）	Systolic blood pressure （kPa）	Diastolic blood pressure （kPa）	Heart rate (beats/min)
Control
Before administration	4	18.9±6.0	22.3±5.2	17.3±4.3	150.3±24.1
60 min after	4	19.8±5.4	23.6±6.0	18.0±4.6	155.0±29.6
90 min after	4	21.0±6.1	23.0±5.2	17.6±4.5	150.8±27.5
150 min after	4	20.3±5.4	22.6±6.3	17.9±5.0	144.5±15.2
180 min after	4	19.9±5.4	22.8±6.1	17.8±5.1	160.5±30.3
300 min after	4	19.4±5.4	23.1±6.3	17.9±5.2	168.5±32.6
GL (4.86 mg/kg)
Before administration	4	20.6±6.1	23.5±6.4	18.2±6.4	189.0±30.1
60 min after	4	20.8±5.6	23.1±6.1	18.3±6.3	187.3±44.0
90 min after	4	20.5±6.5	23.0±6.5	18.5±6.3	182.5±38.6
150 min after	4	21.5±5.2	23.2±5.3	18.5±6.2	180.0±19.3
180 min after	4	21.1±5.5	23.4±5.6	18.2±6.5	192.8±30.8
300 min after	4	20.5±7.3	23.2±5.8	18.5±6.3	193.8±26.5
GL (14.6 mg/kg)
Before administration	4	18.6±5.6	22.3±6.9	16.2±5.4	154.0±32.1
60 min after	4	16.7±5.9	20.3±5.6	14.5±6.7	151.8±49.6
90 min afterP	4	16.9±5.7	20.4±5.9	14.2±6.2	152.0±60.1
150 min after	4	18.0±5.1	21.1±5.8	15.5±6.3	157.0±45.4
180 min after	4	18.1±5.6	20.9±6.2	15.1±6.0	155.3±38.8
300 min after	4	18.2±5.9	20.6±6.3	15.2±6.0	158.0±47.1
GL (43.7 mg/kg)
Before administration	4	19.2±5.5	23. 2±4.9	17.6±5.1	168.0±58.0
60 min after	4	19.4±6.1	22.3±5.6	17.5±5.4	156.8±39.8
90 min after	4	19.3±5.6	21.5±4.9	17.1±5.2	151.5±34.7
150 min after	4	19.1±5.5	21.1±4.8	16.9±5.6	147.8±33.5
180 min after	4	19.0±5.7	21.5±5.2	16.6±5.7	164.0±52.0
300 min after	4	19.0±5.5	22.0±5.7	17. 2±5.0	157.3±48.6
Xingling Granule
Before administration	4	17.1±1.3	20.5±5.6	15.8±6.2	151.3±27.2
60 min after	4	13.1±2.9*	17.9±5.4	10.5±4.3	130.8±10.9
90 min after	4	13.0±3.3	17.7±5.5	10.3±4.3	135.5±11.6
150 min after	4	13.4±2.8*	17.7±5.3	10.4±4.2	144.3±19.8
180 min after	4	13.6±2.7*	18.3±5.1	10.3±4.5	142.3±26.7
300 min after	4	15.1±1.9	19.6±5.2	12.9±4.7	144.8±31.9

Tab 2

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Effects of ginkgolide on cerebral blood flow in dogs

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