消炎灵口服液中黄芩苷含量的测定

doi:10.3736/jcim20050116

Abstract

Objective

To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction.

Methods

HPLC method was used for determination. A Kromosil C₁₈ column was used. The mobile phase consisted of MeOH-acid water (H₂O∶H₃PO₄=53∶0.2) 40∶60, and the detective wavelength was 277 nm.

Results

The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%).

Conclusion

The content of baicalin in Xiao-yanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

Key words: Xiaoyanling Decoction, Baicalin, High-performance liquid chromatography

CLC Number:

R284.1

Jun-yi Jiang, Xiang Li, Rong-li Qiu, Hai-xia Zhang, Zhi-ping Wu. Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography. Journal of Chinese Integrative Medicine, 2005, 3(1): 54-56.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050116

http://www.jcimjournal.com/EN/Y2005/V3/I1/54

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Figures/Tables 3

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References 3

[1]	李学兰, 杨素云 . 清热消炎合剂的制备与临床应用[J]. 山东医药工业, 2000,19(6):46-47
[2]	钟晋 . 紫外分光光度法测定复方金银花片中黄芩苷的含量[J]. 重庆医学, 2004,33(6):959-960
[3]	方滢芝, 齐藓红 . HPLC法测定复方芩芍口服液中黄芩苷的含量[J]. 中药新药与临床药理, 2003,14(2):119-120

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Sample	Added （mg）	Tested （mg）	Recovery rate （%）	Average （%）	RSD （%）
1	0.389 6	0.880 15	102.1	9.12	2.7
2	0.389 6	0.878 86	101.7
3	0.480 7	0.971 80	100.4
4	0.480 7	0.952 20	96.4
5	0.584 4	1.056 52	98.2
6	0.584 4	1.042 88	95.9

Lot	Sample	Content of baicalin （mg/ml）	Average （mg/ml）	RSD （%）
	1	0.482 49
021106	2	0.469 32	0.475 89	1.30
	3	0.475 86
	4	0.467 17
021107	5	0.466 50	0.469 68	1.05
	6	0.475 37
	7	0.495 83
021108	8	0.454 10	0.454 77	2.36
	9	0.444 38
	10	0.411 11
021109	11	0.400 91	0.406 20	1.26
	12	0.406 59
	13	0.489 80
021110	14	0.500 18	0.497 97	1.47
	15	0.503 94
	16	0.459 46
021111	17	0.462 27	0.459 54	0.59
	18	0.456 88

Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography

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