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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (5): 480-485.doi: 10.3736/jcim20100513

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture on expression of obestatin in hypothalamus of rats with simple obesity

 Xian-juan Konga,b, Lei Gaoc, Hao Pengc, Xian Shia   

  1. a Department of Acupuncture and Moxibustion, General Hospital of Peopled Liberation Army, Beijing 100853, China
    b Health Section, August First Physical Culture and Sports Team of Peopled Liberation Army, Beijing 100091, China
    School of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
  • Received:2010-03-31 Accepted:2010-04-21 Online:2010-05-20 Published:2010-09-15
  • Contact: Xian Shi E-mail:301sxian@sina.com

Objective

To investigate the effects of electro-acupuncture on obestatin in rat with simple obesity.
Methods

Obesity was induced in male SD rats with high-fat and -glucose diet for 10 weeks. Twenty-four rats with obesity were randomly divided into untreated group and electro-acupuncture group, with 12 rats in each group. Another 12 normal SD rats serving as controls were fed standard rat chow. Rats in the electro-acupuncture group were treated with electro-acupuncture on bilateral Zusanli (ST36), Tianshu (ST25) and Pishu (BL20) acupoints for fifteen days consecutively. Rats in the blank control group and untreated group were not interfered. Body weight was measured every 3 days during treatment. After treatment, obestatin in serum and hypothalamus was detected by radioimmunoassay or immunohistochemiscal method respectively. Visceral fat weight was also detected.
Results

Body weight and visceral fat weight of rats in the untreated group were significantly higher than those in the blank control group (P<0.01). Body weight and increase of body weight in the electro-acupuncture group were significantly lower than those in the untreated group (P<0.01). Visceral fat weight in the electro-acupuncture group was significantly lower than that in the untreated group (P<0.01), but there was no significant difference as compared with the blank control group. The obestatin expression in hypothalamus was higher in the untreated group than in the blank control group (P<0.05), while it was lower in the untreated group than in the electro-acupuncture group (P<0.01).
Conclusion

Electro-acupuncture has an effect against obesity in rats, which may be related to up-regulating obestatin expression in hypothalamus.

Key words: Simple obesity, Electro-acupuncture, Hypothalamus, Obestatin, Rats

Table 1

Food intake in different groups (x±s, g/d)"

Group n Food intake
Day 1 Day 3 Day 6 Day 9 Day 12 Day 15
Blank control 12 32. 37±3. 71 34. 58±2.00 35. 02±2. 82 36. 68±3. 70 35.38±2.72 34.05±2. 24
Untreated 12 26. 80±2. 74* * 31. 18±3. 55“ 34. 38±2. 81 37. 75±2. 89 40. 20±3. 76* * 40. 28±2. 97 * *
Electro-acupuncture 12 27. 97±4. 93* * 25. 98±1. 74* * AA 24. 86±1. 98* * AA 28. 92±4. 11* * A 30. 07±4. 13* * AA 26.55±2. 68*

Table 2

Body weight and visceral fat weight in different groups (x±s, g)"

Group Body weight Visceral £ftt weight
Before experiment After experiment Difference
Blank control 12 612,11士42,33 673,00 土 43,36“ SO, 88 土 U, 20 25,93土2,45
Untreated 12 764, 93土30,05 ?? 801,84 土 32,13 36,92 土 12,65 ?? 38,07±S,35〃
Electro-acupuncture 12 759,45 土 29,49 ?? 733,抖土23,38 -(25, 55土 19,30) 27,29士2,63^^

Table 3

Content of obestatin in serum and expression of obestatin in hypothalamus in rats (x±s)"

Group n Obestiim content in serum (ng/L) Obestain expreasion in hypothalamus
Blank control 12 1,34 土 0,10 0,33土0,03
Untreated 12 1, 54 土0,21 ? 0,36土0,03?
Electro-acupuncture 12 1,59土0,20〃 0,40±0,04maa

Figure 2

Expression of obestatin in hypothalamus in different groups observed by immunohistochemical method (Light microscopy, ×400) A: PBS control group; B: Blank control group; C: Untreated group; D: Electro-acupuncture group."

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