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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 681-685.doi: 10.3736/jcim20070616

• Original Experimental Research • Previous Articles     Next Articles

Inhibition effects of compound leech eye drops on apoptosis of lens epithelial cells and expressions of Bcl-2 and Bax genes in rats

Xiu-rong Huang, Ming-xin Qi, Zhao-yang Wang, Yi Chen   

  1. Research Center of Pathophysiology,Fujian College of Traditional Chinese Medicine,Fuzhou,Fujian Province 350003,China
  • Online:2007-11-20 Published:2007-11-15

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Objective: To investigate the inhibition effects of compound leech eye drops (Co-SZ) on apoptosis of lens epithelial cells (LECs) induced by hydrogen peroxide (H2O2) and the expressions of apoptosis-related genes Bcl-2 and Bax in rats.Methods: All fresh transparent LECs in SD rats were bathed in culture medium with H2O2 in vitro, meanwhile Co-SZ were added in the culture medium. All LECs were incubated for 24 hours. The apoptosis rate of LECs was determined by terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling method (TUNEL). The changes of LEC ultrastructure and the formation of apoptotic body were observed by transmission electron microscopy. The expressions of apoptosis-related genes Bcl-2 and Bax were detected by streptavdin-peroxidase-biotin method.Results: The apoptosis rate of LECs in the Co-SZ-treated group was significantly lower than that in the H2O2-treated group. The changes of apoptotic LEC ultrastructure in the Co-SZ-treated group were less than those in the H2O2-treated group. The expression of Bcl-2 protein was up-regulated and the expression of Bax protein was down-regulated in the Co-SZ-treated group as compared with the H2O2-treated group.Conclusion: The LEC apoptosis induced by H2O2 can be inhibited by Co-SZ. The molecular mechanisms of Co-SZ in inhibiting LEC apoptosis may be related to regulating the expressions of apoptosis-related genes Bcl-2 and Bax.

Key words: leeches, epithelial cells, cataract, apoptosis, genes

CLC Number: 

  • R776.1

Figure 1

Apoptosis of lens epithelial cells (TUNEL, ×400)A: Blank control group; B: H2O2-treated group; C: Co-SZ-treated group."

Table 1

Effect of Co-SZ eye drops on apoptosis rate of lens epithelial cells injured by H2O2 ($\bar{x}±S$, %)"

Group n Apoptosis rate t
Blank control 6 3.50±1.84
H2O2-treated 6 92.00±2.55** 68.94
Co-SZ-treated 6 38.50±8.56△△ 14.67

Figure 2

Changes of lens epithelial cell ultrastructure (transmission electron microscopy)"

Figure 3

Morphological changes of expression of apoptosis-related gene Bcl-2 in lens epithelial cells regulated by Co-SZ (SP, ×400)"

Figure 4

Morphological changes of expression of apoptosis-related gene Bax in lens epithelial cells regulated by Co-SZ (SP, ×400)"

Table 2

Effect of Co-SZ on expression of Bcl-2 protein in lens epithelial cells injured by H2O2"

Group n – + ++ +++ ++++ Ridit
Blank control 6 0 0 1 1 4 0.970
H2O2-treated** 6 5 1 0 0 0 0.500
Co-SZ-treated△△ 6 0 0 0 4 2 0.935

Table 3

Effect of Co-SZ on expression of Bax protein in lens epithelial cells injured by H2O2"

Group n – + ++ +++ ++++ Ridit
Blank control 6 4 2 0 0 0 0.000
H2O2-treated* 6 0 0 0 2 4 0.500
Co-SZ-treated△△ 6 3 2 1 0 0 0.055
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