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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 686-691.doi: 10.3736/jcim20070617

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Effect of scalp acupuncture on inflammatory response in rats with acute cerebral ischemia-reperfusion injury

Hong-xing Zhang, Ling-guang Liu, Li Zhou, Hao Huang, Xuan Li, Min Yang   

  1. Department of Acupuncture & Moxibustion , Wuhan Chinese and Western Medicine Hospital , Wuhan , Hubei Province 430022 ,China
  • Online:2007-11-20 Published:2007-11-15

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Objective To explore the mechanism of scalp acupuncture (SA) in treating cerebral ischemia.Methods: Sixty SD rats with middle cerebral artery occlusion (MCAO) were randomized into untreated group and SA-treated group, with another group of 10 SD rats without artery occlusion as sham-operated control. Neurological severity score (NSS), hemetoxylin and eosin (HE) staining and enzyme-linked immunosorbent assay were applied to observing the changes of neurofunctional defect, inflammatory infiltration in cerebral tissue and content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-10 at 24, 48 and 72 h after ischemia-reperfusion.Results: There existed significant difference in NSS between SA-treated group and untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The HE staining results of ischemic cerebral tissue showed an apparent reduction of inflamed lesions in SA-treated group as compared with the untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The content of TNF-α and IL-1β at each phase point after ischemia-reperfusion in SA-treated was decreased as compared with that in the untreated group, and there were differences between SA-treated group and untreated group at 72 h after ischemia-reperfusion (P<0.01). An apparent increase was observed in IL-10 between SA-treated group and untreated group at each phase point after ischemia-reperfusion, and there were significant differences between SA-treated group and untreated group at 48 and 72 h after ischemia-reperfusion (P<0.05, P<0.01).Conclusion: Scalp acupuncture can improve neurofunctional rehabilitation, suppress leukocyte infiltration, decrease the content of TNF-α and IL-1β within a certain range and enhance IL-10 expression so as to suppress cytokines-mediated inflammatory reaction and attenuate cerebral ischemia-reperfusion injury.

Key words: scalp acupuncture, brain ischemia, reperfusion, inflammation, rats

CLC Number: 

  • R743.31

Table 1

Comparison of neurological severity score among different groups ($\bar{x}±S$)"

Group n NSS
Sham-operated 10 0
Untreated
24 h after ischemia-reperfusion 10 2.30±0.67**
48 h after ischemia-reperfusion 10 2.10±0.57**
72 h after ischemia-reperfusion 10 2.00±0.47**
SA-treated
24 h after ischemia-reperfusion 10 1.90±0.57**△△
48 h after ischemia-reperfusion 10 1.70±0.48**△△
72 h after ischemia-reperfusion 10 1.30±0.48**△△

Table 2

Number of HE stained inflammatory cells in different groups ($\bar{x}±S$)"

Group n Inflammatory cell number
Sham-operated 10 1.10±0.56
Untreated
24 h after ischemia-reperfusion 10 12.50±1.27**
48 h after ischemia-reperfusion 10 18.10±1.37**
72 h after ischemia-reperfusion 10 11.10±1.20**
SA-treated
24 h after ischemia-reperfusion 10 12.30±1.25**
48 h after ischemia-reperfusion 10 12.10±1.20**△△
72 h after ischemia-reperfusion 10 7.00±1.41**△△

Figure 1

Brain tissue in different groups (HE staining, ×400)A: Sham-operated group; B: Untreated group at 24 h after ischemia-reperfusion; C: Untreated group at 48 h after ischemia-reperfusion; D: Untreated group at 72 h after ischemia-reperfusion; E: SA-treated group at 24 h after ischemia-reperfusion; F: SA-treated group at 48 h after ischemia-reperfusion; G: SA-treated group at 72 h after ischemia-reperfusion."

Table 3

Plasma content of TNF-α, IL-1β and IL-10 in rats with brain ischemia-reperfusion in different groups (ng/L, $\bar{x}±S$)"

Group n TNF-α IL-1β IL-10
Sham-operated 10 4.94±0.97 56.34±6.31 64.02±9.80
Untreated
24 h after ischemia-reperfusion 10 7.69±1.44** 66.29±9.78** 138.86±14.66**
48 h after ischemia-reperfusion 10 9.13±1.83* 74.04±7.80** 134.27±13.93**
72 h after ischemia-reperfusion 10 11.37±1.31** 64.12±9.01* 166.55±11.04**
SA-treated
24 h after ischemia-reperfusion 10 6.51±1.36** 65.65±6.31* 143.36±10.76**
48 h after ischemia-reperfusion 10 8.24±1.62** 73.44±6.87** 151.67±15.50**△
72 h after ischemia-reperfusion 10 7.08±1.63**△△ 52.57±7.79△△ 196.26±26.02**△△

Table 4

Brain content of TNF-α, IL-1β and IL-10 in rats with brain ischemia-reperfusion in different groups (ng/L, $\bar{x}±S$)"

Group n TNF-α IL-1β IL-10
Sham-operated 10 75.41±10.33 99.31±7.37 95.16±15.20
Untreated
24 h after ischemia-reperfusion 10 104.79±9.71** 123.09±5.80** 169.74±23.15**
48 h after ischemia-reperfusion 10 125.68±9.11** 139.06±5.93** 234.54±26.40**
72 h after ischemia-reperfusion 10 145.44±17.96** 131.72±5.34** 204.76±24.59**
SA-treated
24 h after ischemia-reperfusion 10 95.31±9.76** 118.90±7.70** 179.80±24.84**
48 h after ischemia-reperfusion 10 117.67±10.69** 135.67±4.02** 276.43±30.35**△△
72 h after ischemia-reperfusion 10 121.74±11.69**△△ 102.69±7.96△△ 235.96±23.47**△△
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