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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 41-44.doi: 10.3736/jcim20080109

• Original Clinical Research • Previous Articles     Next Articles

Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis

Dong Yang1, Yong-ping Du1(), Qing Shen2, Wei Chen3, Yan Yu3, Guang-lei Chen3   

  1. 1. Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
    2. Department of Nephrology, Paediatrics Hospital, Fudan University, Shanghai 210000, China
    3. Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
  • Received:2007-05-15 Online:2008-01-20 Published:2008-01-15
  • Contact: DU Yong-ping

Objective:To investigate the expression of α-smooth muscle action (α-SMA) in the renal tubulointerstitium in patients with kidney collateral stasis.

Methods:The expression of α-SMA in the renal biopsy specimens from 54 patients with kidney collateral stasis was examined by immunohistochemical method.

Results:The degree of kidney collateral stasis was increased with the increasing of the degree of renal interstitial fibrosis (P<0.05), and there was significant positive correlation between kidney collateral stasis and α-SMA expression (P<0.05).

Conclusion:Kidney collateral stasis is one of the main reasons of renal fibrosis. With the increasing of kidney collateral stasis, MFBs in the renal interstitium proliferate obviously, becoming one of the most important causes of renal interstitial fibrosis.

Key words: nephrosis, fibrosis, alpha actin, fibroblasts

CLC Number: 

  • R692

Table 1

Index of blood stasis, expression of α-SMA in the renal tubulointerstitium and the content of serum creatinine and blood urea nitrogen in different groups (?x±s)"

Group n Index of blood stasis Expression of α-SMA Level of serum creatinine (μmol/L) Level of blood urea nitrogen (mmol/L)
Light fibrosis 22 3.14±0.56 9.10±5.48 74.81±18.9 5.03±1.55
Moderate fibrosis 16 4.87±0.89* 15.00±6.69* 107.40±42.98 7.32±3.56
Severe fibrosis 16 6.63±0.86*△ 31.58±7.16*△ 267.54±178.19*△ 12.38±6.03*△

Figure 1

Pathology characteristics of renal biopsy specimens under microscope (Masson staining, ×200) A: Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

Figure 2

Expression of α-SMA in the renal tubulointerstitium under microscope (Immunohistochemical staining, ×200) Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

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