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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 123-127.doi: 10.3736/jcim20050212

• Original Experimental Research • Previous Articles     Next Articles

Apoptosis-promoting effect of Panax notoginseng extracts on MNNG-transformed GES-1 cells

Jun-xiang Li1,Zhi-bin Wang1,Ling-qun Zhu2,Fu-ling Niu2,Wei Cui2   

  1. 1. Department of Gastroenterology, Dongfang Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100078, China
    2. Laboratory of Internal Medicine, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China
  • Online:2005-03-20 Published:2005-03-20
  • Contact: Jun-xiang Li E-mail:lijx1226@263.net

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Objective

To study the apoptosis-promoting effect of the serum from Panax notoginseng extracts-fed dog on precancerous gastric cells by means of flow cytometry.

Methods

In the experiment, we adopted eternalized human gastric mucosa epithelium GES-1 cells transformed by N-methyl-N’-nitro-N-nitroso-guanidine (MNNG) (MC cells) as the model of precancerous lesions for study in vitro. We took the serum of a dog before and at two different points of time (2 and 6 hours) after feeding the dog with Panax notoginseng extracts for experiment. The MC cells were cultured in mediums with different concentrations of the medicated serum at 2- or 6-hour point of time for 72 hours. By means of flow cytometry, we examined the apoptosis-promoting effects of the serums on the MC cells.

Results

The medicated serums at these 2 points of time had significant effects in promoting MC cell apoptosis. The proportions of apoptotic cells in culture mediums with medicated serums had a significant increase as compared with those in culture mediums with non-medicated serums (serum obtained before administration of extracts to the dog) under the same conditions (P<0.05). The number of MC cells in G0/G1 phase was decreased (P<0.05) and that in G2/M phase increased (P<0.05), while no consistent changes were observed in S phase.

Conclusion

The medicated serums obtained at the two different points of time have significant apoptosis-promoting effects on MC cells. They decrease the number of MC cells in G0/G1 phase and increase the number of MC cells in G2/M phase. This is probably responsible for the effects of Panax notoginseng extracts in inhibiting the proliferation of MC cells and promoting its apoptosis.

Key words: Panax notoginseng, Gastric cancer, Cell culture, Apoptosis, Animals, Laboratory

CLC Number: 

  • R285.6

Fig 1

Flow cytometric analysis of MC cell apoptosis in normal control medium"

Fig 2

Flow cytometric analysis of MC cell apoptosis in mediums with different kinds of serumsA: Culture medium with 8％ of non-medicated serum; B: Culture medium with 4％ of non-medicated serum; C: Culture medium with 8% of medicated serum at 2 h; D: Culture medium with 4% of medicated serum at 2 h; E: Culture medium with 8% of medicated serum at 6 h;F: Culture medium with 4% of medicated serum at 6 h."

Tab 1

Apoptotic rate of MC cells cultured in different mediums ($\bar{x}$±s)"

Culture medium n Apoptotic rate of MC cells (%)
Normal control medium 6 0.71±0.36
Culture medium with 8% of non-medicated serum 6 1.09±0.40
Culture medium with 4％ of non-medicated serum 6 2.27±1.10*
Culture medium with 8％ of medicated serum at 2 h 6 13.13±1.32*▲
Culture medium with 4% of medicated serum at 2 h 6 5.76±1.21*△
Culture medium with 8％ of medicated serum at 6 h 6 11.24±2.28*▲
Culture medium with 4％ of medicated serum at 6 h 6 6.02±1.47*△

Tab 2

Effects of different culture mediums on MC cell cycles ($\bar{x}$±s)"

Culture medium n MC cells in different cell cycles (%)
G0/G1 G2/M S
Normal control medium 6 54.76±1.24 9.96±1.99 35.27±0.87
Culture medium with 8% of non-medicated serum 6 45.48±2.54* 6.86±0.96 47.67±2.76
Culture medium with 4％ of non-medicated serum 6 46.12±3.92* 17.58±2.72* 36.30±1.30*
Culture medium with 8％ of medicated serum at 2 h 6 27.43±1.22*▲ 40.11±4.29*▲ 32.46±2.26▲
Culture medium with 4% of medicated serum at 2 h 6 28.84±3.66*△ 39.79±6.12*△ 31.36±2.48*△
Culture medium with 8％ of medicated serum at 6 h 6 30.36±1.90*▲ 35.23±6.97*▲ 33.94±4.46▲
Culture medium with 4％ of medicated serum at 6 h 6 35.97±1.16*△ 26.42±1.03* 37.61±1.43

Fig 3

DNA analysis of cell cycles of MC cells cultured in normal control medium"

Fig 4

DNA analysis of cell cycles of MC cells cultured in mediums with different kinds of serumsA: Culture medium with 8％ of non-medicated serum; B: Culture medium with 4％ of non-medicated serum; C: Culture medium with 8% of medicated serum at 2 h; D: Culture medium with 4% of medicated serum at 2 h; E: Culture medium with 8% of medicated serum at 6 h; F: Culture medium with 4% of medicated serum at 6 h."

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