Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (7): 821-826.doi: 10.3736/jcim20120714

• Original Experimental Research • Previous Articles     Next Articles

Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes

Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu()   

  1. Key Laboratory of Chinese Internal Medicine of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2011-12-21 Accepted:2012-01-04 Online:2012-07-20 Published:2018-07-15
  • Contact: Hong-fang Liu E-mail:lhfdoctor@sohu.com

Objective: To investigate the effects of Astragalus membranaceus and Potentilla discolor mixture (APM) on insulin resistance (IR) and mRNA expressions of IR-related genes, including phosphatidylinositol 3-kinase (PI3-K), phosphoenolpyruvate carboxykinase (PEPCK) and peroxisome proliferator-activated receptor γ coactivator 1 (PGC1) in KKAy mice with early type 2 diabetes and to explore the gene regulation mechanisms of AMP.
Methods: After giving short-term high-fat and high-calorie diet to induce type 2 diabetes, male KKAy mice were randomly divided into model and APM groups. Nine C57BL/6J mice were used as normal control in addition. The mice in the APM group were treated with 830 g/L of the APM liquid by gastric infusion while the mice in the model group and the normal control group were given 0.05% sodium carboxymethyl cellulose at a dose of 0.1 mL/g body weight once per day. After four weeks, fasting plasma glucose (FPG) was tested using tail vein blood. Fasting serum insulin (FINS) was tested by radioimmunoassay. Insulin sensitivity index (ISI) was calculated as the natural logarithm of the product of FPG and FINS. The mRNA expressions of PI3-K, PEPCK and PGC1 in liver tissues were tested by real-time fluorescence quantitative polymerase chain reaction assay.
Results: Both the levels of FPG and FINS in the model group and the APM group were increased, while the ISI values were decreased when compared to those of the normal control group (P<0.01). The level of FPG in the APM group was decreased, while ISI was increased when compared to those of the model group (P<0.05). All of the mRNA expressions of PI3-K, PEPCK, and PGC1 in liver tissue of the model group were decreased compared with the normal control group (P<0.01). The mRNA expressions of PI3-K and PGC1 in the liver tissue of the APM group were higher than those in the model group (P<0.05).
Conclusion: APM can improve the insulin resistance of mice with type 2 diabetes. The mechanism may be related to increasing the mRNA expressions of PI3-K and PGC1 in the liver tissue.

Key words: drugs, Chinese herbal, diabetes mellitus, type 2, diabetes mellitus, experimental, insulin resistance, 1-phosphatidylinositol 3-kinase, phosphoenolpyruvate carboxylase, peroxisome proliferator-activated receptor γ, mice

Table 1

Levels of FPG, FINS and ISI after four weeks in each group (x±s)"

Group n FPG (mmol/L) FINS (μIU/mL) ISI
Normal control 9 7.07±1.13 4.81±2.16 –3.50±0.45
Model 9 28.56±5.47** 134.03±50.57** –8.27±0.41**
APM 9 17.97±5.75**△ 78.84±34.35** –7.11±0.55**△

Table 2

mRNA expressions of PI3-K, PEPCK and PGC1 in liver tissue of mice in each group (x±s)"

Group n PI3-K mRNA PEPCK mRNA PGC1 mRNA
Normal control 3 1.11±0.23 1.07±0.15 1.1±0.27
Model 3 0.54±0.18** 0.43±0.21** 0.19±0.04**
APM 3 0.72±0.12 0.39±0.21 0.27±0.07
[1] Lin H, Ding XJ . The possible mechanism of insulin resistance in type 2 diabetes[J]. Shi Yong Yu Fang Yi Xue, 2008,15(1):285-286
doi: 10.3969/j.issn.1006-3110.2008.01.128
林慧, 丁晓娟 . 2型糖尿病胰岛素抵抗的可能发生机理[J]. 实用预防医学, 2008,15(1):285-286
doi: 10.3969/j.issn.1006-3110.2008.01.128
[2] Wan XD, Chen BS, Xia YZ, Wang XM . Effects of PKC on signal transduction in palmitate-induced hepatic insulin resistance[J]. Zhongguo Lao Nian Xue Za Zhi, 2008,28(2):115-116
doi: 10.3969/j.issn.1005-9202.2008.02.005
万学东, 陈宝生, 夏炎枝, 王西明 . PKC在软脂酸诱导的肝细胞胰岛素抵抗信号转导中的作用[J]. 中国老年学杂志, 2008,28(2):115-116
doi: 10.3969/j.issn.1005-9202.2008.02.005
[3] Guo XM, Cui RJ . Effect of herbal Potentilla discoloris on insulin and thymus index of rat model of type 2 diabetes mellitus[J]. Mudanjiang Yi Xue Yuan Xue Bao, 2004,25(4):1-4
郭新民, 崔荣军 . 翻白草对2型糖尿病大鼠血清胰岛素和胸腺指数的影响[J]. 牡丹江医学院学报, 2004,25(4):1-4
[4] Mao XQ, Yu F, Wang N, Wu Y, Zou F, Wu K, Liu M, Ouyang JP . Hypoglycemic effect of polysaccharide enriched extract of Astragalus membranaceus in diet induced insulin resistant C57BL/6J mice and its potential mechanism[J]. Phytomedicine, 2009,16(5):416-425
doi: 10.1016/j.phymed.2008.12.011
[5] Zhu YF, Li HH, Cui RJ . Effect of herb Potenillae discoloris on immune function of rat model with type 2 diabetes mellitus[J]. Mudanjiang Yi Xue Yuan Xue Bao, 2010,31(1):5-6
朱雁飞, 李怀慧, 崔荣军 . 翻白草对2型糖尿病大鼠免疫功能的影响[J]. 牡丹江医学院学报, 2010,31(1):5-6
[6] Liu HF, Wang KL . Experimental study of effects of insulin resistance on liver tissue in early type 2 diabetes[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2007,13(3):208-210
柳红芳, 王克林 . 2型糖尿病发病前期胰岛素抵抗对肝脏组织影响的实验研究[J]. 中国中医基础医学杂志, 2007,13(3):208-210
[7] Luo L, Yin HJ, Zhang Y, Jiang YR, Liu Y, Shi DZ . Effect of ginseng fruit saponins on insulin sensitivity index in high fat-fed rats[J]. J Chin Integr Med, 2005,3(6):463-466
罗兰, 殷惠军, 张颖, 蒋跃绒, 刘颖, 史大卓 . 人参果总皂苷对高脂饲养大鼠胰岛素敏感指数的影响[J]. 中西医结合学报, 2005,3(6):463-466
[8] Li SZ, annotated by Zhang SK . Compendium of materia medica[M]. Beijing: Chinese Press of Traditional Chinese Medicine, 1998: 702
李时珍编著, 张守康校注 . 本草纲目[M]. 北京: 中国中医药出版社, 1998: 702
[9] Huang CL, Lü YP . Effects of auxiliary treatment with Astragalus mongholicus on insulin resistance of type 2 diabetes[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2003,23(10):779-782
黄春玲, 吕玉萍 . 黄芪辅助治疗2型糖尿病对胰岛素抵抗的影响[J]. 中国中西医结合杂志, 2003,23(10):779-782
[10] Foster FM, Traer CJ, Abraham SM, Fry MJ . The phosphoinositide(PI) 3-kinase family[J]. J Cell Sci, 2003,116(Pt 15):3037-3040
doi: 10.1242/jcs.00609
[11] Bennett WL, Keeton AB, Ji S, Xu J, Messina JL . Insulin regulation of growth hormone receptor gene expression: involvement of both the PI-3 kinase and MEK/ERK signaling pathways[J]. Endocrine, 2007,32(2):219-226
doi: 10.1007/s12020-007-9021-2
[12] Zhao WH, Xiao JZ, Yang WY, Wang N, Wang X, Chen XP, Bu S . Relationship between adipose phosphoenolpyruvate carboxykinase and occurrence and reversion of insulin resistance[J]. Zhonghua Yi Xue Za Zhi, 2005,85(43):3074-3080
赵文惠, 萧建中, 杨文英, 王娜, 王昕, 陈晓平, 卜石 . 脂肪磷酸烯丙醇羧激酶与胰岛素抵抗的发生和逆转的关系[J]. 中华医学杂志, 2005,85(43):3074-3080
[13] Qu DM, Song GY, Gao Y, Wang J, Hu SG, Han M . Expression pattern of PGC-1α and Mfn2 in insulin resistance state and after recovery in rat[J]. Ji Chu Yi Xue Yu Lin Chuang, 2008,28(2):133-138
曲东明, 宋光耀, 高宇, 王敬, 胡淑国, 韩梅 . 胰岛素抵抗大鼠及恢复状态下PGC-1α和Mfn2表达的变化[J]. 基础医学与临床, 2008,28(2):133-138
[1] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[2] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[3] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[4] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[5] Bérénice Colleville, Nicolas Perzo, Guillaume Avinée, Anaïs Dumesnil, Frederic Ziegler, Paul Billoir, Hélène Eltchaninoff, Vincent Richard, Eric Durand. Impact of high-fat diet and vitamin D3 supplementation on aortic stenosis establishment in waved-2 epidermal growth factor receptor mutant mice. Journal of Integrative Medicine, 2019, 17(2): 107-114.
[6] Mehranghiz Ebrahimi-Mameghani, Mohammad Asghari-Jafarabadi, Khatereh Rezazadeh. TCF7L2-rs7903146 polymorphism modulates the effect of artichoke leaf extract supplementation on insulin resistance in metabolic syndrome: A randomized, double-blind, placebo-controlled trial. Journal of Integrative Medicine, 2018, 16(5): 329-334.
[7] Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[8] Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[9] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[10] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[11] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[12] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[13] Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[14] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[15] Tom Fleischer, Tung-Ti Chang, Hung-Rong Yen. Post-hematopoietic stem cell transplantation in patients with hematologic disorders: Chinese herbal medicine for an unmet need. Journal of Integrative Medicine, 2016, 14(5): 322-335.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[2] Ya-hui Liang , Ping Li, Jing-xia Zhao, Xin Liu, Qi-fu Huang. Acetyl-11-keto-beta-boswellic acid and arsenic trioxide regulate the productions and activities of matrix metalloproteinases in human skin fibroblasts and human leukemia cell line THP-1. Journal of Chinese Integrative Medicine, 2010, 8(11): 1060-1069
[3] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[4] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[5] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[6] Stephen D. Anton, Jonathan Shuster, Christiaan Leeuwenburgh. Investigations of botanicals on food intake, satiety, weight loss and oxidative stress: Study protocol of a double-blind, placebo-controlled, crossover study. Journal of Chinese Integrative Medicine, 2011, 9(11): 1190-1198
[7] Xiang-yu Kong, Yuan Hao, Tai-xiang Wu, Yan-ming Xie. Adverse drug reactions or adverse events of Chaihu Injection: A systematic review. Journal of Chinese Integrative Medicine, 2010, 8(12): 1124-1132
[8] Jian-sheng Li, Su-yun Li, Xue-qing Yu, Yang Xie. Bufei Yishen Granule combined with acupoint-sticking therapy in patients with stable chronic obstructive pulmonary disease: Study protocol of a multicenter, randomized, double-blind, active-controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(12): 1312-1318
[9] Wei Zhang, Hai-yu Liu. Variabilities of the impacts of cold pathogen and cold-dampness pathogen on fractalkine mRNA expression in lung tissues of rats. Journal of Chinese Integrative Medicine, 2008, 6(2): 171-174
[10] Li-hui Dang, Xiao-nan Yang, Qing Xia. Protective effects of Chaiqin Chengqi Decoction on isolated pancreatic acinar cells in acute pancreatitis rats and the mechanisms. Journal of Chinese Integrative Medicine, 2008, 6(2): 176-179