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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 918-924.doi: 10.3736/jcim20120813

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Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency

Zi-hui Li1, Jian Wang1(), Rong-lin Cai2, You-wen Wang3, Jian-peng Hu4   

  1. 1. School of Clinical Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    2. Institute of Acupuncture, Moxibustion, and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    3. School of Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    4. Graduate School of Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2012-01-16 Accepted:2012-04-04 Online:2012-08-20 Published:2018-08-15
  • Contact: Jian Wang E-mail:wangjian6301@163.com

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Objective: To establish a rat model of ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy.

Methods: Sixty rats were divided into normal control group, ulcerative colitis group, ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy group (model group) and strengthening spleen for resolving dampness group. Ulcerative colitis in rats was induced by enema containing trinitrobenzene sulfonic acid (TNBS) and ethanol. The rats in the model group were suffered from standing in water, limiting sleeping time and abnormal diet based on administration of TNBS and ethanol. The rats in the spleen strengthening and dampness resolving group were gastrically administered with Shenlin Baizhu Powder, a compound traditional Chinese herbal medicine. Symptoms, signs and pathological changes in colon tissue of rats were observed after modeling. The levels of interleukin (IL)-6, IL-8 and tumor necrosis factor-α (TNF-α) in serum of rats were measured by enzyme-linked immunosorbent assay.

Results: The rats in the model group showed lethargy, poor appetite, loss of energy, diarrhea and bloody stool. Their body weight decreased significantly compared with the normal control group, and similar changes were found in the comparison of food intake, drinking amount, urine amount, stool wet weight and assay of spontaneous activity (P<0.05). When observed under a light microscope, the colon tissues of rats in the model group showed mucosal edema, congestion, inflammatory cell infiltration and ulceration. The degree of colon injury and IL-6, IL-8 and TNF-α levels were significantly increased (P<0.05) as compared to those in the normal control group. The changes mentioned above were improved by Shenlin Baizhu Powder (P<0.05).

Conclusion: The rat model of ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy is successfully induced and has the characteristics of ulcerative colitis of humans both in pathological changes and in syndrome.

Key words: ulcerative colitis, syndrome of dampness stagnancy due to spleen deficiency, disease models, animal, rats

Table 1

The score criteria of colon injury degree"

Score Colon injury degree
0 No injury
1 Mucosal hyperaemia and edema, no ulcer
2 Mucosal hyperaemia, edema and mild erosion, no ulcer
3 Mucosal hyperaemia, edema and moderate erosion, with single ulcer
4 Mucosal hyperaemia, edema and severe erosion, with multiple ulcers
5 Mucosal hyperaemia, edema and severe erosion, area of the ulcers >1 cm2

Table 2

Comparison of macroscopic quantization indexes among four groups of rats （$\bar{x}$±s）"

Group n Body
weight (g)		 Food intake
in 24 h
(g/kg weight)		 Drinking amount
in 24 h
(g/kg weight)		 Urine amount
in 24 h
(g/kg weight)		 Stool wet
weight in 24 h
(g/kg weight)		 Assay of
spontaneous
activity
Normal control 15 271.8±30.2 112.3±12.8 195.7±21.3 40.5±5.9 31.8±3.7 901.3±62.7
TNBS model 15 245.4±20.3**△ 101.4±16.7*△ 147.5±18.7**△ 31.4±3.1** 50.6±6.4** 595.2±52.3**△△
Internal and
external factors
plus TNBS model		 14 208.8±21.7** 92.5±18.9** 120.5±15.6** 29.8±3.5** 58.4±6.8** 458.4±47.1**
Shenling Baizhu
Powder		 15
 258.9±27.8△△ 107.8±13.2△△ 161.7±17.5△△ 39.8±4.7△△ 33.7±4.2△△ 756.7±56.5△△

Table 3

Comparison of the score of colon injury among four groups of rats （$\bar{x}$±s）"

Group n Score of colon injury
Normal control 15 0.79±0.25
TNBS model 15 3.68±0.84**△
Internal and external factors plus TNBS model 14 4.82±0.96**
Shenling Baizhu Powder 15 1.21±0.50△△

Figure 1

Colon tissue of rats stained with hematoxylin and eosin (Light microscopy, ×200) A: Normal control group; B: Trinitrobenzene sulfonic acid group; C: Internal and external factors plus trinitrobenzene sulfonic acid group; D: Shenling Baizhu Powder group. "

Table 4

Levels of IL-6, IL-8 and TNF-α in serum of rats in different groups （$\bar{x}$±s, ng/L）"

Group n IL-6 IL-8 TNF-α
Normal control 15 27.51±2.78 34.78±3.86 0.89±0.10
TNBS model 15 37.82±5.65** 51.21±6.38** 1.69±0.15**
Internal and external factors plus TNBS model 14 39.64±4.23** 51.62±5.49** 1.87±0.19**
Shenling Baizhu Powder 15 29.01±3.28△△ 40.65±4.02*△△ 1.02±0.11△△
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